Collagen‐derived peptides modulate CD4⁺ T‐cell differentiation and suppress allergic responses in mice

Abstract

IntroductionCollagen peptides have been widely used as a food supplement. After ingestion of collagen peptides, oligopeptides containing hydroxyproline (Hyp), which are known to have some physiological activities, are detected in peripheral blood. However, the effects of collagen‐peptide administration on immune response are unclear. In the present study, we tested the effects of collagen‐peptide ingestion on allergic response and the effects of collagen‐derived oligopeptides on CD4+ T‐cell differentiation.MethodsBALB/c mice fed a collagen‐peptide diet were immunized with ovalbumin (OVA), and their serum IgE and IgG levels, active cutaneous anaphylaxis, and cytokine secretion by splenocytes were examined. Naive CD4+ T cells were stimulated with anti‐CD3 and anti‐CD28 in the presence of collagen‐derived oligopeptides, and the expression of IFN‐γ, IL‐4, and Foxp3 was analyzed.ResultsIn an active anaphylaxis model, oral administration of collagen peptides suppressed serum OVA‐specific immunoglobulin E (IgE) production and diminished anaphylaxis responses. In this model, the ingestion of collagen peptides skewed the pattern of cytokine production by splenocytes toward T‐helper (Th) type 1 and regulatory T (Treg) cells. In vitro T‐helper cell differentiation assays showed that Hyp‐containing oligopeptides promoted Th1 differentiation by upregulating IFN‐γ‐induced signal transducer and activator of transcription 1 (STAT1) signaling. These oligopeptides also promoted the development of Foxp3+ Treg cells in response to antigen stimulation in the presence of TGF‐β.ConclusionsCollagen‐peptide ingestion suppresses allergic responses by skewing the balance of CD4+ T cells toward Th1 and Treg cells and seems to be a promising agent for preventing allergies and inflammatory diseases.

High Throughput Identification of Antihypertensive Peptides from Fish Proteome Datasets

“…Previous studies also reported that collagen subunits contain multiple host defense peptides [ 47 ]. What is more, some collagen-derived peptides have antioxidant and anti-inflammatory capacities [ 48 ], and even can suppress allergic responses [ 49 ]. In this present study, multiple collagen subunits aroused our attention since their mapping ratios are much higher compared with other proteins.…”

From Food Waste to Innovative Biomaterial: Sea Urchin-Derived Collagen for Applications in Skin Regenerative Medicine

“…Therefore, a collagen-based biomaterial can be considered the best in mimicking the ECM microenvironment [7]. Moreover, due to its biodegradability, collagen can be easily tuned/modified/substituted by cells of the neo-forming tissue and its degradation within the regenerating tissue does not lead to toxic compounds/molecules but rather helps tissue restoration since collagen peptides are considered bioactive molecules [8][9][10]. For all these reasons, collagen-based skin-like scaffolds (CBSS), such as Integra ® and Matriderm ® , which are designed to temporarily or permanently close the wound, are among the most commonly used in biomedicine [11].…”

Collagen supplementation for skin health: A mechanistic systematic review

“…13 Also, this finding is repeated in other studies on different immune system-related diseases. 11,22,23 As mentioned above, the beneficial effects of intact or hydrolyzed form of collagen on skin health were reported by many recent studies.…”

“…In this regard, a surprising tip is several reports of some researches on skin autoimmune defects about the immune response suppression against extracellular matrix after collagen oral administration 13 . Also, this finding is repeated in other studies on different immune system‐related diseases 11,22,23 …”

“…As mentioned above, the beneficial effects of intact or hydrolyzed form of collagen on skin health were reported by many recent studies. Although several mechanisms are reported for both effects and side effects of collagen intervention, 11,22 the exact mechanisms are unknown. In this systematic review, we evaluated the studies that used collagen supplementation for improving skin health parameters to find out if there are inconsistencies in this regard.…”