Collagen–chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells

Natesan, Shanmugasundaram; Ravichandran, Praveen Kumar; Reddy, P. Neelakanta; Ramamurty, Nalini; Pal, Subrata; Rao, K. Panduranga

doi:10.1016/s0142-9612(00)00220-9

Cited by 320 publications

(200 citation statements)

References 16 publications

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“…A very broad endothermic peak in the 107-225 °C region was again observed similar to Construct I, due to the loss of bound water from the gelatinous matrix. Similar results have been observed for collagen-GAG blends due to phase transitions and water loss thus confirming the formation of the constructs [105,106].…”

Section: Differential Scanning Calorimetrysupporting

confidence: 87%

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Romanelli

Knoll

Santora

et al. 2015

Fibers

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Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its avascular nature. Herein we have developed two new hybrid polypeptide-glycosaminoglycan microfibrous scaffold constructs and compared their abilities to stimulate cell adhesion, proliferation, sulfated proteoglycan synthesis and soluble collagen synthesis when seeded with chondrocytes. Both constructs were designed utilizing self-assembled Fmoc-protected valyl cetylamide nanofibrous templates. The peptide components of the constructs were varied. For Construct I a short segment of dentin sialophosphoprotein followed by Type I collagen were attached to the templates using the layer-by-layer approach. For Construct II, a short peptide segment derived from the integrin subunit of Type II collagen binding protein expressed by chondrocytes was attached to the templates followed by Type II collagen. To both constructs, we then attached the natural polymer N-acetyl glucosamine, chitosan. Subsequently, the glycosaminoglycan chondroitin sulfate was then attached as the final layer. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), atomic force microscopy and scanning electron microscopy. In vitro culture studies were carried out in the presence of chondrocyte cells for OPEN ACCESSFibers 2015, 3 266 both scaffolds and growth morphology was determined through optical microscopy and scanning electron microscopy taken at different magnifications at various days of culture. Cell proliferation studies indicated that while both constructs were biocompatible and supported the growth and adhesion of chondrocytes, Construct II stimulated cell adhesion at higher rates and resulted in the formation of three dimensional cell-scaffold matrices within 24 h. Proteoglycan synthesis, a hallmark of chondrocyte cell differentiation, was also higher for Construct II compared to Construct I. Soluble collagen synthesis was also found to be higher for Construct II. The results of the above studies suggest that scaffolds designed from Construct II be superior for potential applications in cartilage tissue regeneration. The peptide components of the constructs play an important role not only in the mechanical properties in developing the scaffolds but also control cell adhesion, collagen synthesis and proteoglycan synthesis capabilities.

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Section: Differential Scanning Calorimetrysupporting

confidence: 87%

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Romanelli

Knoll

Santora

et al. 2015

Fibers

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“…[4][5][6] In addition, IPNs have been created from proteins and polysaccharides, manufactured to take advantage of various properties including biocompatibility and degradability. [7][8][9] Naturally derived protein scaffolds are of interest in the field of tissue engineering because of their biological and physiological relevance. In particular, collagen type I and fibrin have been used individually as protein scaffolds in biomaterial applications because of their demonstrated ability to interact with embedded cells.…”

Section: Introductionmentioning

confidence: 99%

Interpenetrating Collagen-Fibrin Composite Matrices with Varying Protein Contents and Ratios

2006

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Pure and composite hydrogel matrices of collagen type I and fibrin were produced by simultaneous polymerization of each biopolymer in the presence of vascular smooth muscle cells. The ratio of collagen to fibrin in composite matrices was varied from 1:1 to 1:4, with corresponding absolute protein concentrations of 1.0-5.0 mg/mL. Constructs cultured for 7 days were subjected to uniaxial tensile testing, analysis of cell content, as well as scanning electron and confocal microscopic imaging. Gel compaction over time in culture decreased with increasing protein content but was augmented by the presence of fibrin. Material properties (modulus, ultimate tensile stress, and toughness) were highly correlated with gel compaction, protein density, and cell concentration. Maximum force at failure was dependent on absolute protein concentration. This study examined the interrelationships between protein type, ratio, and density in composite biopolymer matrices and contributes to the understanding of structure-function relationships in such materials.

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“…12 In a previous study, nonporous chitosan membranes were modified with chondroitin sulfate (CSA) and CSAchitosan membranes were evaluated for their ability to support chondrogenesis.7,11 In a separate study, a scaffold in the form of an interpenetrating polymeric network was made from a mixture of collagen and chitosan. 13 The biocompatibility of the chitosan-based scaffolds have been evaluated in mice,14 where porous chitosan scaffolds (unseeded) were implanted in mice, and animals were sacrificed after 1, 2, 4, 8, or 12 weeks. Macroscopic inspection of the implantation site revealed no pathological inflammatory responses, gram staining and limulus assays revealed no evidence of infection or endotoxin, and lymphocyte proliferation assays and antibody responses indicated a low incidence of chitosan-specific reactions.14 This study serves to demonstrate that chitosanbased scaffolds had a high degree of in vivo biocompatibility in the animal model studied.…”

Section: Introductionmentioning

confidence: 99%

Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation

Subramanian¹,

Lin²

2005

J Biomedical Materials Res

138

103

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Collagen–chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells

Cited by 320 publications

References 16 publications

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Interpenetrating Collagen-Fibrin Composite Matrices with Varying Protein Contents and Ratios

Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation

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