Collagen-based biocomposites inspired by bone hierarchical structures for advanced bone regeneration: ongoing research and perspectives

Qin, Di; Wang, Na; You, Xin-Guo; Zhang, An-Di; Chen, Xiguang; Liu, Ya

doi:10.1039/d1bm01294k

Cited by 45 publications

(31 citation statements)

References 292 publications

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“…Furthermore, Figure 7E shows that BMSCs cultured with 1:10 SWNTs/EDC show a capillary-like appearance, but control cells show only a scattered pattern ( Figures 7A,B ). This result may be associated with the superior biocompatibility and rough surface topography of SWNTs and SWNTs/EDC composites ( Qin et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes

Luo

Zheng

et al. 2022

Front. Bioeng. Biotechnol.

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Carbon nanotubes (CNTs) are a promising bioactive scaffold for bone regeneration because of their superior mechanical and biological properties. Vascularization is crucial in bone tissue engineering, and insufficient vascularization is a long-standing problem in tissue-engineered scaffolds. However, the effect of CNTs on vascularization is still minimal. In the current study, pristine single-walled carbon nanotubes (SWNTs) were purified to prepare different ratios of SWNTs/EDC composites, and their surface morphology and physicochemical properties of SWNTs/EDC were studied. Furthermore, the effect of SWNTs/EDC on vascularization was investigated by inducing the differentiation of bone mesenchymal stem cells (BMSCs) into vascular endothelial cell-like cells (VEC-like cells). Results showed that SWNTs/EDC composite was successfully prepared, and EDC was embedded in the SWNTs matrix and uniformly distributed throughout the composites. The AFM, FTIR spectra, and Raman results confirmed the formation of SWNTs/EDC composites. Besides, the surface topography of the SWNTs/EDC composites presents a rough surface, which may positively affect cell function. In vitro cell culture revealed that SWNTs and SWNTs/EDC composites exhibited excellent biocompatibility and bioactivity. The SWNTs/EDC composite at mass/volume ratios 1:10 had the best enhancement of proliferation and differentiation of BMSCs. Moreover, after culture with SWNTs/EDC composite, approximately 78.3% ± 4.2% of cultured cells are double-positive for FITC-UEA-1 and DiI-Ac-LDL double staining. Additionally, the RNA expression of representative endothelial cell markers VEGF, VEGF-R2, CD31, and vWF in the SWNTs/EDC composite group was significantly higher than those in the control and SWNTs group. With the limitation of our study, the results suggested that SWNTs/EDC composite can promote BMSCs differentiation into VEC-like cells and positively affect angiogenesis and bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes

Luo

Zheng

et al. 2022

Front. Bioeng. Biotechnol.

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“…Furthermore, bone diseases like osteoporosis exacerbate mineral loss from the centrum, causing the bone–implant interface to be weaker . Therefore, different biomaterials like hydrogel, collagen, calcium phosphates, and bioactive glass were investigated as bone cement material to induce bone ingrowth. Although these materials are bioactive, they failed to replace PMMA in vertebroplasty due to their poor mechanical properties for the spine.…”

Section: Discussionmentioning

confidence: 99%

Biological Fixation of Bioactive Bone Cement in Vertebroplasty: The First Clinical Investigation of Borosilicate Glass (BSG) Reinforced PMMA Bone Cement

Zhang

Cui

Zhuo

et al. 2022

ACS Appl. Mater. Interfaces

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PMMA bone cement has been clinically used for decades in vertebroplasty due to its high mechanical strength and satisfactory injectability. However, the interface between bone and PMMA is fragile and more prone to refracture in situ because PMMA lacks a proper biological response from the host bone with minimal bone integration and dense fibrous tissue formation. Here, we modified PMMA by incoporating borosilicate glass (BSG) with a dual glass network of [BO 3 ] and [SiO 4 ], which spontaneously modulates immunity and osteogenesis. In particular, the BSG modified PMMA bone cement (abbreviated as BSG/PMMA cement) provided an alkaline microenvironment that spontaneously balanced the activities between osteoclasts and osteoblasts. Furthermore, the trace elements released from the BSGs enhanced the osteogenesis to strengthen the interface between the host bone and the implant. This study shows the first clinical case after implantation of BSG/PMMA for three months using the dual-energy CT, which found apatite nucleation around PMMA instead of fibrous tissues, indicating the biological interface was formed. Therefore, BSG/PMMA is promising as a biomaterial in vertebroplasty, overcoming the drawback of PMMA by improving the biological response from the host bone.

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“…[ 63,64 ] One of the key requirements of bone tissue engineering is the design and development of tissue engineering scaffolds that recapitulate important characteristics of bone to mimic native bone tissue function and therefore regenerate the damaged tissues. [ 65–67 ] In addition to the antibacterial and biocompatibility of Ti 3 C 2 MXene, they have Ca 2+ binding sites which promote osteogenesis. [ 68,69 ] The integrated Ti 3 C 2 MXenes with 3D‐printed bioactive glass scaffolds have been utilized for bone tissue regeneration and cancer treatment.…”

Section: Regenerative Medicinementioning

confidence: 99%

Biomedical Applications of MXene‐Integrated Composites: Regenerative Medicine, Infection Therapy, Cancer Treatment, and Biosensing

Maleki

Ghomi

Nikfarjam

et al. 2022

Adv Funct Materials

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MXenes (viz., transition metal carbides, carbonitrides, and nitrides) have emerged as a new subclass of 2D materials. Due to their outstanding physicochemical and biological properties, MXenes have gained much attention in the biomedical field in recent years, including drug delivery systems, regenerative medicine, and biosensing. Additionally, the incorporation of MXenes into hydrogels has garnered significant interest in biomedical engineering as an electroactive and mechanical nanoreinforcer capable of converting nonconductive scaffolds into excellent conductors of electricity with an impressive effect on mechanical properties for the engineering of electroactive organs and tissues such as cardiac, skeletal muscle, and nerve. However, many questions and problems remain unresolved that need to be answered to usher these 2D materials toward their true destiny. Thus, this review paper aims to provide an overview of the design and applications of MXene-integrated composites for biomedical applications, including cardiac tissue engineering, wound healing, infection therapy, cancer therapy, and biosensors. Moreover, the current challenges and limitations of utilizing MXenes in vivo are highlighted and discussed, followed by its prospects as a guideline toward possible various futuristic biomedical applications. This review article will inspire researchers, who search for properties, opportunities, and challenges of using this 2D nanomaterial in biomedical applications.

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Collagen-based biocomposites inspired by bone hierarchical structures for advanced bone regeneration: ongoing research and perspectives

Abstract: Bone is a hard-connective tissue composed of matrix, cells and bioactive factors with hierarchical structure, where the matrix is mainly composed of type I collagen and hydroxyapatite. Collagen fibers assembled...

Cited by 45 publications

References 292 publications

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes

Biological Fixation of Bioactive Bone Cement in Vertebroplasty: The First Clinical Investigation of Borosilicate Glass (BSG) Reinforced PMMA Bone Cement

Biomedical Applications of MXene‐Integrated Composites: Regenerative Medicine, Infection Therapy, Cancer Treatment, and Biosensing

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