Collagen Autoantibodies and Their Relationship to CCP Antibodies and Rheumatoid Factor in the Progression of Early Rheumatoid Arthritis

Whittingham, Senga; Stockman, A.; Rowley, Merrill J.

doi:10.3390/antib6020006

Cited by 6 publications

(5 citation statements)

References 81 publications

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“…As generally accepted, RF and anti-CCP antibodies are important markers for the diagnosis and prognosis of RA ( 53 ). Numerous clinical data have shown that RF is not specific for RA diagnosis, and approximately 30% to 45% of patients with RA do not have RF ( 54 ).…”

Section: Resultsmentioning

confidence: 99%

Circular RNA circNUP214 Modulates the T Helper 17 Cell Response in Patients With Rheumatoid Arthritis

et al. 2022

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Circular RNAs (circRNAs) are important transcriptional regulators of genome expression that participate in the pathogenesis of human diseases. Mechanistically, circRNAs, as competitive endogenous RNAs (ceRNAs), can sponge microRNAs (miRNAs) with miRNA response elements. A previous study identified that hsa_circ_0089172 (circNUP214) is abnormally expressed in Hashimoto’s thyroiditis. However, the role of circNUP214 in rheumatoid arthritis (RA) remains unclear. In total, 28 RA patients and 28 healthy controls were enrolled in this study. We found that circNUP214 is an abundant and stable circRNA in RA patients that can potentially differentiate RA patients from healthy subjects. Additionally, the elevated levels of IL-23R positively correlated with circNUP214 expression. The knockdown of circNUP214 resulted in the reduction of IL-23R at both transcriptional and translational levels in human CD4+ T cells. The proportion of circulating Th17 cells and the transcript levels of IL-17A were increased in RA patients and were both positively correlated with IL-23R expression. Moreover, positive correlations between the transcript levels of circNUP214 and the percentage of Th17 cells and the transcript levels of IL-17A were observed in RA patients. The downregulation of circNUP214 decreased the proportion of Th17 cells and the transcript levels of IL-17A in vitro. Furthermore, circNUP214 functioned as a ceRNA for miR-125a-3p in RA patients. Taken together, our results indicate that elevated levels of circNUP214 contribute to the Th17 cell response in RA patients.

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Section: Resultsmentioning

confidence: 99%

Circular RNA circNUP214 Modulates the T Helper 17 Cell Response in Patients With Rheumatoid Arthritis

et al. 2022

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“…CII activates innate and adaptive immune responses, which have a primary role in the initiation and pathogenesis of RA in CIA model. Some studies found that anti-CII antibodies are present in the serum and synovial fluid of RA patients precede the onset of joint symptoms ( Mullazehi et al, 2007 ; Whittingham et al, 2017 ). Patients with positive anti-CII antibodies exhibited higher disease activity and more severe symptoms ( Mullazehi, et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Tripterygium wilfordii glycosides ameliorates collagen-induced arthritis and aberrant lipid metabolism in rats

Zhu

Zhang

et al. 2022

Front. Pharmacol.

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Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, and the dysregulation of lipid metabolism has been found to play an important role in the pathogenesis of RA and is related to the severity and prognosis of patients. Tripterygium wilfordii glycosides (TWG) is extracted from the roots of Tripterygium wilfordii Hook F. with anti-inflammatory and immunosuppressive effects, and numerous clinical trials have supported its efficacy in the treatment of RA. Some evidence suggested that TWG can modulate the formation of lipid mediators in various innate immune cells; however whether it can improve RA-related lipid disorders has not been systematically studied. In the study, type Ⅱ collagen-induced arthritis (CIA) model was used to investigate the efficacy of TWG in the treatment of RA and its effect on lipid metabolism. Paw volume, arthritis score, pathological changes of ankle joint, serum autoantibodies and inflammatory cytokines were detected to assess the therapeutic effect on arthritis in CIA rats. Then, shotgun lipidomics based on multi-dimensional mass spectrometry platform was performed to explore the alterations in serum lipidome caused by TWG. The study showed that TWG could effectively ameliorate arthritis in CIA rats, such as reducing paw volume and arthritis score, alleviating the pathological damages of joint, and preventing the production of anti-CII autoantibodies and IL-1β cytokine. Significant increase in ceramide and decrease in lysophosphatidylcholine were observed in CIA rats, and were highly correlated with arthritis score and IL-1β level. After TWG treatment, these lipid abnormalities can be corrected to a great extent. These data demonstrate that TWG exerts a beneficial therapeutic effect on aberrant lipid metabolism which may provide new insights for further exploring the role and mechanism of TWG in the treatment of RA.

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“…2 Auto-reactive B cell produces anti-cyclic citrullinated peptides antibody (ACPA), rheumatoid factor (RF) and anti-type II collagen (CII). [3][4][5] ACPA is found to be about 95% of RA cases and has the highest specificity among the three autoantibodies. 6,7 ACPA is primarily noticed as the persistent protein in the joint or circulation that contributes to the formation of the immune complex causes inflammation and joint destruction.…”

Section: Introductionmentioning

confidence: 96%

Effect of Hyperbaric Oxygen Exposure on Bacterial Toxin Antigen and Collagen Induced Arthritis in Animal Model

Harnanik¹,

Soeroso²,

Suryokusumo³

et al. 2019

Nep. Med. Coll. J.

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Anti-cyclic citrullinated peptide antibody (ACPA) is a specific autoantibody that binds with citrullineamino acid in rheumatoid arthritis (RA). Interleukin-17a (IL-17a) is one of the cytokines that play an important role in chronic inflammation during the process of autoimmune diseases. Bacterial toxin antigen and collagen induced arthritis (ACIA) is the gold standard of RA animal model. The aim of this study was to see the effect of hyperbaric oxygen (HBO) exposure on ACIA animal models. ACIA model was made by combined antigens (bacterial toxin and collagen). A total of 24 male Balb/Cmice modelled on ACIA were divided into three groups. Eight mice did not receive HBO exposure (control group, indicated as G1), eight mice (indicated as G2) received HBO exposure for 10 days while remaining eight mice (indicated as G3) for five days. G2 mice were exposed to HBO 2.4 ATA oxygen 100% for 90 minutes (30 minutes each with two intervals of five minutes breathing with normal air) for 10 consecutive days while G3 mice were exposed only for five days. The indicators of arthritis i.e. ACPA and IL-17a were measured by enzyme-linked immunosorbent assay (ELISA) technique. A significant decrease in ACPA and IL-17a levels was seen in both HBO exposed groups (G2 and G3) compared to G1 (p<0.05). There was no significant difference in levels of ACPA and IL-17a in G3 mice and G2 mice (p > 0.05). HBO reduced inflammation in ACIA by decreasing ACPA andIL-17a levels through improvement of hypoxic conditions and showed therapeutic potential for the treatment of RA.

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Collagen Autoantibodies and Their Relationship to CCP Antibodies and Rheumatoid Factor in the Progression of Early Rheumatoid Arthritis

Cited by 6 publications

References 81 publications

Circular RNA circNUP214 Modulates the T Helper 17 Cell Response in Patients With Rheumatoid Arthritis

Circular RNA circNUP214 Modulates the T Helper 17 Cell Response in Patients With Rheumatoid Arthritis

Tripterygium wilfordii glycosides ameliorates collagen-induced arthritis and aberrant lipid metabolism in rats

Effect of Hyperbaric Oxygen Exposure on Bacterial Toxin Antigen and Collagen Induced Arthritis in Animal Model

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