Collagen as a Biomaterial for Skin and Corneal Wound Healing

Sklenářová, Renáta; Akla, Naoufal; Latorre, Meagan Jade; Ulrichová, Jitka; Franková, Jana

doi:10.3390/jfb13040249

Cited by 32 publications

(20 citation statements)

References 171 publications

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“…Lastly, during the maturation step, cells are engaged in the secretion of molecules involved in the extracellular matrix remodeling, such as collagen type I, which represents the most abundant protein synthetized and secreted by fibroblasts [ 29 ]. As collagen I secretion increased after SS administration, both in a standard and in an inflamed-environment condition, the authors assumed that a wound healing process could be definitely facilitated by SS administration.…”

Section: Discussionmentioning

confidence: 99%

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

et al. 2023

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Snail slime (SS) is a viscous secretion obtained from different snail species. SS composition is variable according to factors such as the extraction method. Even if several papers have been published regarding this topic, the molecular mechanisms at the base of SS biological effects remain unexplored. Thus, the aim of this study is to evaluate the capability of SS, extracted with the cruelty-free Muller method, to promote viability and angiogenesis processes and, in parallel, to counteract inflammation occurrence on skin cell populations. SS was administered to keratinocytes, macrophages and fibroblasts, then cell viability, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, cytotoxicity by lactate dehydrogenase (LDH) assay, morphology by haematoxylin-eosin staining, gene and protein expression through real-time polymerase chain reaction (PCR) and Western blot, cell cycle phases by flow cytometry, and collagen secretion using an enzyme-linked immunosorbent assay (ELISA) test, were measured. Our results evidence SS capability to promote fibroblast viability and to trigger recovery mechanisms by activating the Erk protein. Moreover, an appreciable anti-inflammatory effect due to the significant reduction in cyclooxygenase-2 expression, and a positive modulation of new blood vessel formation demonstrated by increased Angiopoietin 1 gene expression and a higher matrix deposition (evidenced by the augmented amount of released collagen I) can be identified. This evidence led us to assume that the Muller method extracted-SS represents a valuable and promising natural product suitable for cosmetic and skin care formulations.

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Section: Discussionmentioning

confidence: 99%

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

et al. 2023

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“…Regarding the latter aspect, the in vitro migration assay performed here demonstrated that Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy) coating has induced a significant improvement in wound healing at 24 h and a complete reconstitution of the cell monolayer after 48 h compared to CTRL, allowing a more aligned and regular orientation of hGF in the direction of wound closure. In support of this evidence, there is a large literature on the beneficial effects of collagen in wound healing in different tissue districts, including the oral ones [ 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 93%

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Romasco

Mandrillo²,

Morsut

et al. 2023

Biomedicines

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Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h (p < 0.05) and wound closure at 24 h (p < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ (p < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.

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“…Knowledge about collagen has been exponentially increased in the past decades. Whereas scientific community in the 1970s talked about only four types of genetically distinct collagens [ 23 ], at present it is generally accepted that collagen superfamily comprises up to 28 members that differ from each other in molecular composition as well as in their supramolecular organization within the ECM [ 24 , 25 , 26 , 27 ]. The occurrence of an additional collagen type, called collagen XXIX, has been also claimed by some authors [ 28 , 29 ], although, given that COL29A1 gene was shown to be identical to the COL6A5 gene and that the a1(XXIX) chain corresponds to the a5(VI) [ 30 ], collagen XXIX is not generally accepted as a genetically distinct family [ 24 ].…”

Section: Understanding Collagen Worldmentioning

confidence: 99%

Collagen Supplementation for Joint Health: The Link between Composition and Scientific Knowledge

et al. 2023

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Osteoarthritis (OA) is the most common joint disease, generating pain, disability, and socioeconomic costs worldwide. Currently there are no approved disease-modifying drugs for OA, and safety concerns have been identified with the chronic use of symptomatic drugs. In this context, nutritional supplements and nutraceuticals have emerged as potential alternatives. Among them, collagen is being a focus of particular interest, but under the same term different types of collagens coexist with different structures, compositions, and origins, leading to different properties and potential effects. The aim of this narrative review is to generally describe the main types of collagens currently available in marketplace, focusing on those related to joint health, describing their mechanism of action, preclinical, and clinical evidence. Native and hydrolyzed collagen are the most studied collagen types for joint health. Native collagen has a specific immune-mediated mechanism that requires the recognition of its epitopes to inhibit inflammation and tissue catabolism at articular level. Hydrolyzed collagen may contain biologically active peptides that are able to reach joint tissues and exert chondroprotective effects. Although there are preclinical and clinical studies showing the safety and efficacy of food ingredients containing both types of collagens, available research suggests a clear link between collagen chemical structure and mechanism of action.

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Collagen as a Biomaterial for Skin and Corneal Wound Healing

Cited by 32 publications

References 171 publications

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

Snail Slime Extracted by a Cruelty Free Method Preserves Viability and Controls Inflammation Occurrence: A Focus on Fibroblasts

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Collagen Supplementation for Joint Health: The Link between Composition and Scientific Knowledge

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