Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach

Heidenreich, Ana Carolina; Pérez-Recalde, Mercedes; Wusener, Ana González; Hermida, Élida B.

doi:10.1016/j.polymertesting.2019.106297

Cited by 58 publications

(44 citation statements)

References 32 publications

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“…The data extraction of the included studies is presented in Table 1 . The studies were classified depending on the study design, whereas; twelve studies were in vitro [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], two studies were in vivo [ 31 , 32 ], and four studies conducted both [ 33 , 34 , 35 , 36 ]. The characteristics and outcomes of both in vitro and in vivo studies are presented in Table 2 and Table 3 , respectively.…”

Section: Resultsmentioning

confidence: 99%

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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Three-dimensional bioprinting has rapidly paralleled many biomedical applications and assisted in advancing the printing of complex human organs for a better therapeutic practice. The objective of this systematic review is to highlight evidence from the existing studies and evaluate the effectiveness of using natural-based bioinks in skin regeneration and wound healing. A comprehensive search of all relevant original articles was performed based on prespecified eligibility criteria. The search was carried out using PubMed, Web of Science, Scopus, Medline Ovid, and ScienceDirect. Eighteen articles fulfilled the inclusion and exclusion criteria. The animal studies included a total of 151 animals with wound defects. A variety of natural bioinks and skin living cells were implanted in vitro to give insight into the technique through different assessments and findings. Collagen and gelatin hydrogels were most commonly used as bioinks. The follow-up period ranged between one day and six weeks. The majority of animal studies reported that full wound closure was achieved after 2–4 weeks. The results of both in vitro cell culture and in vivo animal studies showed the positive impact of natural bioinks in promoting wound healing. Future research should be focused more on direct the bioprinting of skin wound treatments on animal models to open doors for human clinical trials.

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Section: Resultsmentioning

confidence: 99%

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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“…The presence of the cells inside the ink to utilize a bio-ink provides the possibility to print 3D structures which are often implanted into impaired bone tissue to enhance cell responses [82,[154][155][156][157][158][159][160][161]. CS can be used as a bio ink either in pure form or in combination with other materials, especially other polymers [154,155]. Wu et al [155] prepared the 3D-ink printing of CSat room temperature where the CS inks were fabricated via dissolving in acidic mixture prior to the 3D printing (Table 2).…”

Section: Bio-inksmentioning

confidence: 99%

“…Nevertheless, the use of Col/CS blends as bio-inks is yet hard to find. In an examination conducted by Heidenreich [154], various hydrogels in the form of Col/CS were synthesized to print scaffolds (single-layered) for TE applications owing to its appropriate viscosity, printability as well as concentration (Col/CS ratio). The fabricated bio-inks were stable for 44 h in PBS and did not induce any toxicity to the NIH-3T3 cells.…”

Section: Bio-inksmentioning

confidence: 99%

“…The results also indicated that the concentration of Col in the Col/CS solution has a significant effect on viscosity. In Heidenreich's study, further incorporation of Col into the solution increases the viscosity, enhances cell viability, cell attachment and alters the morphology of the cells [154]. According to Table 2, in another study, a derivative of CS, carboxymethyl chitosan was used in combination with gelatin and sodium alginate as bio-ink containing bone mesenchymal stem cells for 3D bio-printing [156].…”

Section: Bio-inksmentioning

confidence: 99%

“…Due to the fact that clinical purposes of cell-based approaches are restricted as a result of the expense of keeping cellular constructs on the shelf, possible immune reaction to allogeneic cell lines, and scarce tissue, these acellular scaffolds which may stimulate endogenous influx and uniform distribution of native stem cells from bone marrow could be great candidate for cartilage reproduction [ 109 ]. In this connection, chitosan based constructs have been used for soft tissue regeneration frequently [ 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , …”

Section: Chitosan-based 3d Printed Construct For Hard Tissues Applmentioning

confidence: 99%

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Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

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Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

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3D‐Printed and Recombinant Spider Silk Particle Reinforced Collagen Composite Scaffolds for Soft Tissue Engineering

Koeck,

Trossmann,

Scheibel

2024

Adv Funct Materials

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Collagen is one main component of the extracellular matrix (ECM) in natural tissues and is, therefore, well suited as a biomaterial for tissue engineering. In this study, a method is presented to 3D‐bioprint collagen into a precipitation bath comprising recombinantly produced spider silk protein eADF4(C16) yielding a composite with excellent mechanical properties. The spider silk precipitation bath induced assembly of the collagen into fibrils, and subsequent addition of potassium phosphate buffer lead to the formation of silk particles and stabilization of the collagen fibrils. The produced collagen‐silk composite scaffolds show an internal structure of homogeneously distributed and interacting collagen fibrils and spider silk particles with significantly better mechanical properties compared to plain collagen scaffolds. Further, enzymatic degradation assays of the scaffolds over a 7‐day period show higher stability of the collagen‐silk scaffolds compared to plain collagen scaffolds in the presence of wound proteases. Using the spider silk variant eADF4(C16‐RGD) further increases compressive stress and elastic modulus compared to that of the unmodified variant. Finally, it is shown that the unique collagen‐spider silk composite scaffolds comprising the cell‐binding domains of collagen and the RGD sequence in the spider silk variant represent a promising material for soft tissue regeneration.

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Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach

Cited by 58 publications

References 32 publications

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

3D‐Printed and Recombinant Spider Silk Particle Reinforced Collagen Composite Scaffolds for Soft Tissue Engineering

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