Collagen/alginate scaffolds comprising core (PCL)–shell (collagen/alginate) struts for hard tissue regeneration: fabrication, characterisation, and cellular activities

Kim, YongBok; Kim, GeunHyung

doi:10.1039/c3tb20485e

Cited by 75 publications

(40 citation statements)

References 37 publications

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“…Some of these techniques include multiphoton crosslinking 23 , extrusion 24,25 , and inkjet bioprinting 26–28 . Many of these methods utilize materials in addition to collagen when 3D printing, such as polycaprolactone and alginate, to generate constructs with improved mechanical integrity 25,27 . Type-I collagen has been used alone as a bio-ink in different 3D printing strategies.…”

Section: Introductionmentioning

confidence: 99%

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

et al. 2017

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As a biomaterial, collagen has been used throughout tissue engineering and regenerative medicine. Collagen is native to the body, is highly biocompatible, and naturally promotes cell adhesion and regeneration. However, collagen fibers and the inherent weak mechanical properties of collagen hydrogels interfere with further development of collagen as a bio-ink. Herein, we demonstrate the use of a modified type-I collagen, collagen methacrylamide (CMA), as a fibril-forming bio-ink for free-form fabrication of scaffolds. Like collagen, CMA can self-assemble into a fibrillar hydrogel at physiological conditions. In contrast, CMA is photocrosslinkable and thermoreversible, and photocrosslinking eliminates thermoreversibility. Free-form fabrication of CMA was performed through self-assembly of the CMA hydrogel, photocrosslinking the structure of interest using a photomask, and cooling the entire hydrogel, which results in cold-melting of unphotocrosslinked regions. Printed hydrogels had a resolution on the order of ~350 μm, and can be fabricated with or without cells and maintain viability or be further processed into freeze-dried sponges, all while retaining pattern fidelity. A subcutaneous implant study confirmed the biocompatibility of CMA in comparison to collagen. Free-form fabrication of CMA allows for printing of macroscale, customized scaffolds with good pattern fidelity and can be implemented with relative ease for continued research and development of collagen-based scaffolds in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

et al. 2017

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“…The bioactive molecule impregnated with collagen have been used to enhance the stability of the scaffold 14,15 . Collagen scaffolds mimic the function of extracellular matrix (ECM) to support collagen matrix synthesis, cell proliferation 16 and also examines the signs of fibroblast activation 17,18 . Recently bioactive molecules have functionalized with collagen to form thin film or scaffold as a biomaterial 19,20 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and biological evaluation of chromen and pyrano chromen-5-one derivatives impregnated into a novel collagen based scaffold for tissue engineering applications

et al. 2015

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In this article, we describe the synthesis and biological evaluation of a novel 2-(methylamino)-3-nitro-4-(4-oxo-4Hchromen-3-yl) pyrano[3,2-c]chromen-5(4H)-one (CCN). It is also determined that CCN impregnated into the collagen scaffold has the potential to mimic the function of the extracellular matrix as a biomaterial in the field of tissue engineering. The series of pyrano[3,2-c]chromen-5(4H)-one derivatives (4a-4j), were analyzed by 1 H NMR, 13 C NMR, Mass spectral and FTIR analysis. The compound 4c confirmed by single crystal XRD studies. All the compounds were screened for antimicrobial activity against the gram positive, gram negative bacteria and yeast. Among all the compounds, the compound (4a=CCN) showed activity against Gram positive and Gram negative bacteria, when compared to the synthesized compounds. Further the compound CCN was evaluated for cytotoxicity against MCF-7, Hep-2 and Vero cancer cell lines with IC50 values of 5.4 µg/ml, 5.3 µg/ml and 68.4 µg/ml respectively. In addition, the result of flow cytometry and docking ((PDBID: 1A27 with the ligand) study were supported to the activity of the synthesized compound (4a). The FTIR and NMR analysis of the CCN impregnated collagen scaffold were done to reveal the existence of CCN molecule in the scaffold. The inherent property of the collagen scaffold was not significantly affected by structure of CCN molecule. The thermal and mechanical properties of the collagen scaffold impregnated with CCN molecule gives stability as well as supports the swelling. However, the COL-CCN scaffold showed an enhanced cell attachment and proliferation of NIH 3T3 fibroblast cells. Based on the results, the novel CCN molecule impregnated with collagen scaffold has the potential application as a biomaterial in tissue engineering.

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“…1 Generally, the substitutes used in tissue engineering are termed scaffolds and their functions are similar to that of the extracellular matrix (ECM). 6 However, obtaining a consistent structure of the core (PCL)-shell (alginate/collagen) in the whole scaffold was challenging because of the difficult/complex fabricating process using the two natural components (alginate and collagen), and a residual solvent (EDC, 1-ethyl-3-(3dimethylaminopropyl)carbodiimide) of the cross-linking agent of collagen in the composite scaffold can be toxic and may induce carcinogenic effects. Various synthetic polymers and natural materials have been used to fabricate tissue regenerative materials with many three-dimensional (3D) shapes and micro/nano internal pore structures.…”

Section: Introductionmentioning

confidence: 99%

3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

Lee

Seo

et al. 2015

RSC Adv.

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Three-dimensional (3D) biomedical scaffolds that are physically and mechanically similar to regenerated tissues and provide bioactive sites for cultured cell adhesion, growth, and even differentiation have been used widely in various tissue regenerative materials. In this work, we propose new composite scaffolds consisting of poly(3-caprolactone) (PCL), alginate, and phenamil methanesulfonate (PM), manufactured by a combined process involving 3D plotting together with a low-temperature working plate and a versatile coating process. The composite scaffolds consist of microsized struts with a core (PCL)-shell (alginate/PM) structure. The PCL in the micro-sized struts has the function of providing mechanical support to the scaffold, and the shell region (alginate/phenamil) is used as a biologically active material.PM is known to stimulate osteoblastic differentiation and mineralization. However, phenamil cannot be used easily as a biomedical scaffold material because of its low molecular weight and low processability.We first introduced the bioactive component, PM, in a solid-freeform fabricated multi-layered pore structure. To assess the effects of PM, the biocompatibility of the composite scaffolds for bone tissue regeneration was characterized in vitro using preosteoblasts (MC3T3-E1 cells). Cells were distributed more extensively and proliferated to a greater degree on the PCL/alginate-PM scaffold in a limited PM concentration range versus the PCL/alginate scaffold. Specifically, cell viability and ALP activity were high in a composite scaffold containing PM at 3.5 mg, but not higher PM levels (PM: 5.6 mg per scaffold), compared with PCL/alginate scaffolds. These results suggest that the multi-layered PCL/alginate-PM scaffold may be a promising bioactive material for enhancing bone tissue growth, but over a limited range of PM levels.

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Collagen/alginate scaffolds comprising core (PCL)–shell (collagen/alginate) struts for hard tissue regeneration: fabrication, characterisation, and cellular activities

Cited by 75 publications

References 37 publications

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine

Synthesis, characterization and biological evaluation of chromen and pyrano chromen-5-one derivatives impregnated into a novel collagen based scaffold for tissue engineering applications

3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

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