Collaborative Work to Evaluate Toxicity on Male Reproductive Organs by Repeated Dose Studies in Rats : 26)detection of 1, 3-Dinitrobenzene-Induced Histopathological Changes in Testes and Epididymides of Rats With 2-Week Daily Repeated Dosing

Abstract

As part of a collaborative work, male rats were administered 1,3-dinitrobenzene (1,3-DNB) daily at 0, 25 and 50 mg/kg/day from the age of 6 weeks for 4 weeks (4-week exp.), or at 25, 50 and 75 mg/kg/day from the age of 8 weeks for 2 weeks (2-week exp.). After the end of each administration period, all survivors were sacrificed, and their testes and epididymides were removed, weighed and examined histopathologically. The following results were obtained. In the 4-week exp.: At 50 mg/kg/day, the weights of testes and epididymides showed decrease with macroscopic atrophy. The testicular spermatogenic epithelium showed decrease in the number of sperm-spermatocytes, degeneration/necrosis, giant cell formation and vacuolation, reduction in sperm counts also being evident in the ducts of the epididymides. In the 2-week exp.: At 50 and 75 mg/kg/day, the weights of testes and/or epididymides showed decrease with macroscopic atrophy. Several histopathological changes in the testes and epididymides were essentially the same changes as in the group given 50 mg/kg/day in the 4-week exp., with a clear relation. These results indicate that a 2-week administration period is sufficient to detect testicular and epididymal histopathological changes induced by 1,3-dinitrobenzene in male rats.

The Inhibin B (InhB) Response to the Testicular Toxicants Mono‐2‐Ethylhexyl Phthalate (MEHP), 1,3 Dinitrobenzene (DNB), or Carbendazim (CBZ) Following Short‐term Repeat Dosing in the Male Rat

“…Although all tubules were affected, there was variability in the relative amount of tubular atrophy versus degeneration between animals. All of the testicular changes were consistent with Sertoli cell and germ cell injury previously reported following DNB treatment (Blackburn et al, ; Haschek and Rousseaux, ; Irimura et al, ).…”

Electrochemical investigations of antioxidant interactions with radical anion and dianion of 1,3-dinitrobenzene

“…They have also been detected in ground-water near industrial waste disposal sites [5]. 1,3-DNB is a reproductive toxic agent as shown by investigations on rats [6,7]. Antioxidants, as a class of organic compounds, have the ability to terminate chain propagation reactions [8,9].…”

Effects of Stilbene and Related Compounds on Reproductive Organs in B6C3F1/Crj Mouse

“…Marked atrophy of the seminiferous tubules has been observed in the testis of rats exposed to dibromoacetic acid and 1,3-dinitrobenzene, which are thought to be non-estrogenic. 38,39) Estradiol decreased testicular spermatid numbers and epididymal sperm numbers, and caused atrophy of epididymal tubules in male rats given ppm-level dietary exposure. 40) It is also known that neonatal exposure to DES (0.37 mg/kg/day or less for 18 days) can alter the structure of the testicular excurrent ducts, while change in testis weight, distension of the rete testis and efferent ducts, changes of epithelial cell height in the efferent ducts and immunoexpression of the water channel aquaporin-1 have been observed in adult rats.…”