Collaborative study on rat sperm motion analysis using Cellsoft Series 4000 semen analyzer.

Ban, Yoshiki; Naya, Masato; Nishimura, Tōru; Kaneto, Masako; Kishi, Kurajiro; Inoue, Tadahiro; Ooshima, Yôjiro

doi:10.2131/jts.26.9

Cited by 11 publications

(14 citation statements)

References 16 publications

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“…To elucidate the sperm motion parameters that are useful and the most sensitive indicators of the effects of various toxicological chemicals on sperm, collaborative studies in Japan were conducted using the CellSoft Series 4000 semen analyzer and the Hamilton-Thorne Sperm Analyzer (HTM-IVOS), which have nine sperm motion parameters each (Ban et al, 2001;Kato et al, 2001). The CASA system has allowed us to identify toxicant-induced changes in sperm motion that were previously undetected by manual sperm analysis.…”

Section: Introductionmentioning

confidence: 99%

Key Parameters of Sperm Motion in Relation to Male Fertility

et al. 2004

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-This study was undertaken to detect key parameters of rat sperm motion in relation to male fertility by comparing the differences in sperm motion induced by treatment with α-chlorohydrin (ACH), known to produce spermatotoxicity, and nitrobenzene (NTB), known to produce testicular toxicity. Male rats received ACH (5 or 20 mg/kg/day) or NTB (60 mg/kg/day) for either 3 days or 18 days. Epididymal sperm was assessed for motility using a Hamilton-Thorne Sperm Analyzer (HTM-IVOS). Numerical data for statistical analysis and graphical renditions of sperm motion using parameters in radar charts and reconstructed sperm tracks were analyzed to evaluate sperm motion. Males were allowed to copulate with untreated females and cesarean sections were conducted in order to examine the effects of drug administration on male fertility. Linearity of sperm track (linearity (LIN) and/or straightness (STR)) decreased and/or beat cross frequency (BCF) increased only in ACH groups (5 or 20 mg/kg/day), although the percentage of motile sperm, sperm velocities (average path velocity (VAP), curvilinear (VCL), and straight line velocity (VSL)) and amplitude of lateral head displacement (ALH) decreased on Day 18 in both ACH and NTB (60 mg/kg/day) groups. Furthermore, from the individual reconstructed sperm tracks, it was clear that ACH-treated spermatozoa were characterized by abnormal motion ("jerking") with low vigor (low velocities) and little or no forward progression. Finally, only ACH treatment led to a reduction in pregnancy rate or infertility. Therefore, our results suggest that linearity (especially VSL, STR and LIN) in sperm motion is a key parameter for assessing a chemical's potential to induce male infertility.

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Section: Introductionmentioning

confidence: 99%

Key Parameters of Sperm Motion in Relation to Male Fertility

et al. 2004

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“…Therefore, linearity may be a uniquely sensitive indicator of dog sperm motion. ALH max and ALH mean were slightly increased, although not to a significant extent, even though these parameters were decreased in rats by -chlorohydrin or other compounds (Ban et al, 2001). The cause of this different direction of change seems to originate from different shape of sperm between rats and dogs.…”

Section: Discussionmentioning

confidence: 83%

“…VCL or linearity were significantly decreased by -chlorohydrin treatment. In the above-mentioned collaborative study of rats, VCL was one of the most sensitive parameters, but linearity exhibited little or no change on treatment with 9 compounds including -chlorohydrin (Ban et al, 2001). Therefore, linearity may be a uniquely sensitive indicator of dog sperm motion.…”

Section: Discussionmentioning

confidence: 99%

“…Initially, we attempted to measure sperm motion in dogs with the CellSoft-4000 using the conditions for rats in our laboratory (Ban et al, 2001). Sperm of dogs, however, has its own characteristic shape, size and motion, so we could not measure sperm motion in dogs using the conditions appropriate for rats.…”

Section: Discussionmentioning

confidence: 99%

“…We analyzed sperm of rats administered -chlorohydrin with the CellSoft-4000 in a collaborative study (Ban et al, 2001). The collaborative study found useful and sensitive sperm motion parameters for rats among the 9 parameters of the CellSoft-4000.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of usefulness of sperm analyses in dogs for male fertility study.

et al. 2001

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The usefulness of sperm analyses in dogs, including sperm motion analysis, was investigated. Sperm motion analysis was performed with the CellSoft-4000 computer-assisted sperm analysis (CASA) system. First, we examined the conditions for preservation of optimal semen quality. We found that sperm retained more of their motility at 4 than at 37 . Secondly, we observed sperm motion, concentration and morphology in dog semen continuously for 11 weeks. We collected semen samples during the test period, and the samples retained sperm motion, concentration and morphology. Finally, we administrated -chlorohydrin, which decreases rodent sperm motion, at a single oral dose of 100 mg/kg or 150 mg/kg to dogs. Sperm motion was inhibited immediately after -chlorohydrin treatment, and recovered after 2 weeks. None of the experimental animals were sacrificed in the above-mentioned examinations.We thus confirmed that sperm analyses including motion analysis in dogs are useful in male fertility studies.

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Quantitative analysis of sperm plane circular movement in the blue mussels Mytilus edulis, M. trossulus and their hybrids

2011

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Fertilization success of free spawning organisms such as Mytilus species depends on gamete interactions. Therefore, gamete traits such as sperm movement are important for determining fertilization success in free spawning organisms. Since little is known about sperm movement pattern in Mytilus species, the purpose of this study was to investigate sperm movement pattern of blue mussel M. edulis, M. trossulus and their hybrids using computer-assisted sperm movement video analysis. Sperm of all genotypes were found to conduct circular movement in a two-dimensional plane. Furthermore, new sperm movement parameters, real time radius (R), angle change rate (θ) and the center of circular track (O(t)) were developed to verify and quantitatively describe the plane circular movement pattern using software (Image-J) that may be widely applied to sperm movement study in other organisms. Angle change rate was positively correlated to fertilization success. However, no correlation between fertilization and real time radius was detected. Although no interspecific differences were found in the radius, the F¹ (first generation) hybrid sperm had a lower angle change rate than M. edulis and M. trossulus. Published studies have shown that sperm circular movement is more prevalent in aquatic broadcast spawning species than in species with mating behavior or internal fertilization. Therefore, a two-dimensional circular movement pattern in sperm may represent a trait that increases fertilization success for broadcast spawning species by either increasing gamete interaction rate at a small scale and/or avoiding swimming further away from the eggs before sperm detects the chemoattractant gradient.

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Collaborative study on rat sperm motion analysis using Cellsoft Series 4000 semen analyzer.

Cited by 11 publications

References 16 publications

Key Parameters of Sperm Motion in Relation to Male Fertility

Key Parameters of Sperm Motion in Relation to Male Fertility

Investigation of usefulness of sperm analyses in dogs for male fertility study.

Quantitative analysis of sperm plane circular movement in the blue mussels Mytilus edulis, M. trossulus and their hybrids

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