Collaborative Study of the Hemoglobin Repletion Test in Chicks and Rats for Measuring Availability of Iron

Gwendolyn, W; Fritz, J. C.

doi:10.1093/jaoac/54.1.13

Cited by 15 publications

(8 citation statements)

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“…Animal models. Although several animal models have been used for studying iron absorption, the only standardized method that has seen widespread use is the Association of Official Analytical Chemists (AOAC) Rat Hemoglobin Repletion Assay (464)(465)(466). Briefly, weanling rats are fed an iron-deficient diet until they develop IDA and are then switched to the test diets.…”

Section: Estimation Of Iron Bioavailabilitymentioning

confidence: 99%

Biomarkers of Nutrition for Development (BOND)—Iron Review

Lynch¹,

Pfeiffer

Georgieff

et al. 2018

The Journal of Nutrition

235

193

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This is the fifth in the series of reviews developed as part of the Biomarkers of Nutrition for Development (BOND) program. The BOND Iron Expert Panel (I-EP) reviewed the extant knowledge regarding iron biology, public health implications, and the relative usefulness of currently available biomarkers of iron status from deficiency to overload. Approaches to assessing intake, including bioavailability, are also covered. The report also covers technical and laboratory considerations for the use of available biomarkers of iron status, and concludes with a description of research priorities along with a brief discussion of new biomarkers with potential for use across the spectrum of activities related to the study of iron in human health.The I-EP concluded that current iron biomarkers are reliable for accurately assessing many aspects of iron nutrition. However, a clear distinction is made between the relative strengths of biomarkers to assess hematological consequences of iron deficiency versus other putative functional outcomes, particularly the relationship between maternal and fetal iron status during pregnancy, birth outcomes, and infant cognitive, motor and emotional development. The I-EP also highlighted the importance of considering the confounding effects of inflammation and infection on the interpretation of iron biomarker results, as well as the impact of life stage. Finally, alternative approaches to the evaluation of the risk for nutritional iron overload at the population level are presented, because the currently designated upper limits for the biomarker generally employed (serum ferritin) may not differentiate between true iron overload and the effects of subclinical inflammation.

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Section: Estimation Of Iron Bioavailabilitymentioning

confidence: 99%

Biomarkers of Nutrition for Development (BOND)—Iron Review

Lynch¹,

Pfeiffer

Georgieff

et al. 2018

The Journal of Nutrition

235

193

View full text Add to dashboard Cite

show abstract

“…Three methods were used in determining the bioavailability of iron. Relative biological value (RBV), as described by Pla and Fritz (1970), evaluates test iron by comparing its ability to replenish hemoglobin against the ability of iron from ferrous sulfate in anemic animals. The ratio of test iron to iron from ferrous sulfate is computed to produce an equivalent hematological response.…”

Section: Relative Biological Value (Rbv)mentioning

confidence: 99%

“…This curve was obtained by plotting graded iron levels (FeSCL) against final hemoglobin concentrations (Pla and Fritz, 1971). A straight line was obtained through the use of 4 control diets (No.…”

Section: Relative Biological Value (Rbv)mentioning

confidence: 99%

Influence of Fish on the Bioavailability of Plant Iron in the Anemic Rat

Chao

Gordon

1983

The Journal of Nutrition

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The effect of fish protein and fish oil on the utilization of endogenous iron in wheat bran, spinach and soy protein isolate was investigated by using the anemic rat as an animal model. Marine products were substituted for casein and corn oil in the diets of these animals. Hemoglobin regeneration was one criteria used to measure iron uptake. Relative biological values (RBV) were computed from a regression equation obtained from control animals receiving graded levels of FeSO4 X 7H2O. The RBV of iron from plant sources provided in diets containing casein-corn oil versus fish-fish oil were: wheat bran, 123 vs. 111%; spinach, 53 vs. 49%; and soybean isolate, 84 vs. 67%; RBV FeSO4 = 100%. These changes were not significant. The decreases in iron absorption from diets containing marine products was attributed to the fish oil. Absorption of exogenous iron (59Fe) was measured in the same animals after the 14-day repletion period. Assimilation of the 59Fe was highly correlated (r2 = 0.958) with hemoglobin level at time of dosing. Diet composition did not appear to have the same effect on the percentage of 59Fe retained after 110 hours by the rat as compared to levels of hemoglobin regeneration (i.e., RBV). A "meat factor" effect was not shown by substituting fish for casein the diets containing plant iron sources fed anemic rats.

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“…Total body Hb (grams) was calculated as the product of estimated blood volume (.0006 dl/g, from Ramirez et al, 1963), body weight (grams) and Hb concentration (grams/ deciliter). Fritz et al (1970) and Pla and Fritz (1972) beach value is the average for two pigs.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, blood is easily biopsied and hemoglobin concentration is determined simply. Fritz et al (1970), Pla and Fritz (1972) and Amine et al (1972) have determined the relative biological value of a number of Fe compounds using Fedepleted chicks and rats. Bioavailability of Fe from a number of sources for young pigs has been determined by Pickett et al (1961), Harmon et al (1967Harmon et al ( , 1969Harmon et al ( , 1974, Ullrey et al (1973), Ammerman et al (1974), Anderson et al (1974), Furugouri and Kawabata (1975) and Miller (1978).…”

Section: Introductionmentioning

confidence: 99%