Collaborative study of a preparation of human cord serum for its use as a reference in the assay of alphafoetoprotein

Sizaret, P; Breslow, Norman E.; Anderson, Shannon W.

doi:10.1016/0092-1157(75)90048-7

Cited by 40 publications

(4 citation statements)

References 24 publications

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“…Crystalline human AFP, isolated as described by , was used as the standard and yielded a concentration of 90 Mg/ml by the radial immunodiffusion technique (Mancini etal., 1965) and a concentration of 76 Mg/ml by RIA in the international standard preparation 72/225 of the World Health Organization (Sizaret et al, 1975).…”

Section: Methodsmentioning

confidence: 99%

Etiology of human liver cancer: Controlled prospective study in liver cirrhosis

Lėhmann

Wegener

1979

Journal of Toxicology and Environmental Health

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The incidence of primary liver cell carcinoma was investigated in a prospective study over 6 yr and 5 mo in 403 clinically unselected patients derived from a homogeneous population by means of serial determination of alpha 1-fetoprotein (AFP) by radioimmunoassay. The diagnosis of liver cirrhosis was proved in 90% by laparoscopy and/or histology and/or autopsy. The incidence of primary liver cell carcinoma in liver cirrhosis in the clinically studied patients was 4.47%, significantly lower than in the autopsy material (11.03%; p less than or equal to 0.025). In the follow-up study, all patients with increasing AFP concentrations exhibited a primary liver cell carcinoma. A transitory rise of AFP (higher than 50 ng/ml) was observed in 15.1% of patients with liver cirrhosis without primary liver cell cancer. In contrast to the results of animal experiments, this transitory rise of AFP was not followed by malignant transformation of the cirrhotic tissue. Posthepatitic liver cirrhosis was observed in 21.57%, postalcoholic liver cirrhosis in 42.93%, and cryptogenic liver cirrhosis in 27.30%. Liver cirrhosis of other etiology occurred in 8.19%. The incidences of primary liver cell cancer in these 4 groups were 4.94, 4.62, 5.45, and 0%, respectively. These differences are not statistically significant, although in absolute figures postalcoholic liver cirrhosis is the main cause of primary liver cell carcinoma in this sample from West Germany. HBs antigen-positive liver cirrhosis was more often associated with primary liver cell cancer than HBs antigen-negative liver cirrhosis (6.58 versus 3.96%); this difference also is not statistically significant. Observations of larger groups of patients may show a higher risk of developing primary liver cell carcinoma in those with a combination of alcohol abuse and HBs antigenemia and/or acute hepatitis in the history. Patients without these 2 risk factors had an incidence of primary liver cell carcinoma of 2.61%; those with 1 risk factor, 5.77%; and those with both risk factors, 10.71%.

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Section: Methodsmentioning

confidence: 99%

Etiology of human liver cancer: Controlled prospective study in liver cirrhosis

Lėhmann

Wegener

1979

Journal of Toxicology and Environmental Health

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“…Zwischen der 12. und der t6. Beim Erwachsenen ist es normalerweise nur noch in Restmengen < t 0 ngr/ml im Serum nachweisbar (Sizaret, P. et al, 1975). 3000 gg/ml im fetalen Serum den h6chsten Wert und f'~llt gegen Ende der Schwangerschaft stark ab (Norgaard-Pedersen, B., 1973).…”

Section: Alpha-l-fetoprotein (Afp)unclassified

Schwangerschaftsspezifische und schwangerschaftsassoziierte Proteine in der Diagnostik und Verlaufskontrolle maligner Erkrankungen

Bauer

Bohn

1978

Klin Wochenschr

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Serum proteins are described the significance of which for pregnancy or for malignant growth is associated with their drastic increase or initial occurrence. In that immunological non-reaction (tolerance) against foreign antigen is inherent in both of the proceeding, and in that in vitro findings display immunosuppressive properties for the described serum proteins, a function within the scope of altered immunity can be conceived for these proteins. A dual role of the so called pregnancy-specific proteins and the pregnancy-associated proteins would be possible.The purpose of the neoplasma-derived serum proteins could be to block the efferent branch of the immune apparatus. They would occupy antigen determinants of the tumor cells and thereby inhibit the specific reaction mechanism of the effector cells as well as render the tumor invulnerable. The latter would closely resemble the earlier concept of a glycoprotein coat of the tumor cell. In this connection, the human Choriongonadotropin appears to be the best investigated and seems to confirm this model.The serum proteins, present in healthy individuals normally in almost indetectable amounts could, by contrast, acquire the function of blocking the afferent branch of the immune system. That is to say these proteins would be able to annul the recognition mechanism of immunologically competent lymphocytes, and to inhibit sensibilization. The electrophoretic mobility test of the pregnancy-associated ~2glycoprotein as well as the determination of el-fetoprotein receptors on T-lymphocytes are evidence for this concept.

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“…Results of AFP assays are expressed in international units of AFP, in comparison with the WHO AFP standard (25,26).…”

Section: Methodsmentioning

confidence: 99%

α-Fetoprotein Levels in Normal Individuals

Martel¹,

Tuyns²,

Sizaret³

1977

Digestion

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Samples of serum were collected at 6- to 12-month intervals in two groups of healthy Africans and the amount of AFP measured by the radioimmunological method. The amounts present were found to remain fairly constant over time. The significance of this stability is discussed with particular reference of the highest levels of AFP observed in normal individuals in relation to primary liver carcinoma and the potential use of the method as a tool for early detection purposes.

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Collaborative study of a preparation of human cord serum for its use as a reference in the assay of alphafoetoprotein

Cited by 40 publications

References 24 publications

Etiology of human liver cancer: Controlled prospective study in liver cirrhosis

Etiology of human liver cancer: Controlled prospective study in liver cirrhosis

Schwangerschaftsspezifische und schwangerschaftsassoziierte Proteine in der Diagnostik und Verlaufskontrolle maligner Erkrankungen

α-Fetoprotein Levels in Normal Individuals

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