Colitis ulcerosa y bronquiectasias: ¿el tratamiento con tofacitinib podría repercutir en los síntomas respiratorios?

León-Román, Francisco; Mesonero-Gismero, Francisco; Pintado-Cort, Beatriz; López-Jurado, Alfonso López-Frías; Máiz-Carro, Luis; Gorospe‐Sarasúa, Luis; Mercedes-Noboa, Edwin; Asensio-Sánchez, Joaquín; Román, Antonio López-San; Nieto-Royo, Rosa

doi:10.1016/j.arbres.2019.08.013

Cited by 1 publication

(1 citation statement)

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“…Any alteration of physioxia runs the risk of altering the normal balance of oxidative stress, with associated harmful consequences [ 6 ]. Given the importance of investigating how cells and tissues are affected by hypoxia/hyperoxia, well-designed experimental settings are required to monitor the partial pressure of oxygen in culture dishes or in baths for ex vivo tissue preparations [ 7 , 8 , 9 , 10 , 11 , 12 ]. Importantly, in addition to controlling oxygen, the study of cells/tissues of organs physiologically subjected to mechanical stress (e.g., lung, heart, vessels, tendons) also requires the use of experimental settings capable of applying realistic mechanical stimuli, since solid evidence on mechanobiology indicates that mechanical stress considerably modulates cell/tissue function [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental Setting for Applying Mechanical Stimuli to Study the Endothelial Response of Ex Vivo Vessels under Realistic Pathophysiological Environments

Osuna

Ulldemolins

Sanz-Fraile

et al. 2021

Life

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This paper describes the design, construction and testing of an experimental setting, making it possible to study the endothelium under different pathophysiological conditions. This novel experimental approach allows the application of the following stimuli to an ex vivo vessel in a physiological bath: (a) a realistic intravascular pressure waveform defined by the user; (b) shear stress in the endothelial layer since, in addition to the pressure waveform, the flow through the vessel can be independently controlled by the user; (c) conditions of hypo/hyperoxia and hypo/hypercapnia in an intravascular circulating medium. These stimuli can be applied alone or in different combinations to study possible synergistic or antagonistic effects. The setting performance is illustrated by a proof of concept in an ex vivo rabbit aorta. The experimental setting is easy to build by using very low-cost materials widely available. Online Supplement files provide all the technical information (e.g., circuits, codes, 3D printer drivers) following an open-source hardware approach for free replication.

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