Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh

Johura, Fatema Tuz; Tasnim, Jarin; Barman, Indrajeet; Biswas, Sahitya Ranjan; Jubyda, Fatema Tuz; Sultana, Marzia; George, Christine Marie; Camilli, Andrew; Seed, Kimberley D.; Ahmed, Niyaz; Alam, Munirul

doi:10.1186/s13099-020-0345-2

Cited by 44 publications

(35 citation statements)

References 32 publications

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“…Regarding the high incidence of erythromycin- and sulphamethoxazole-resistance, the results of the present study are in agreement with those recently reported [ 44 ] on E. coli isolated from different sources. Although several reports on E. coli highlighted a wide distribution of colistin resistance, frequently mediated by the mobile mcr gene [ 44 ], in the present study, the percentage of resistance against colistin was low. This finding is great of interest because colistin is recognized as the last-resort antibiotic for the treatment of infectious diseases caused by multidrug-resistant (MDR) gram-negative bacteria [ 44 ].…”

Section: Discussionsupporting

confidence: 93%

“…Although several reports on E. coli highlighted a wide distribution of colistin resistance, frequently mediated by the mobile mcr gene [ 44 ], in the present study, the percentage of resistance against colistin was low. This finding is great of interest because colistin is recognized as the last-resort antibiotic for the treatment of infectious diseases caused by multidrug-resistant (MDR) gram-negative bacteria [ 44 ]. Moreover, as recommended by the EUCAST subcommittee [ 45 ], in the present study the resistance to colistin was confirmed by determination of MIC through broth micro-dilution method.…”

Section: Discussioncontrasting

confidence: 76%

“…Hence, the MIC value of 16 mg/L, revealed in the present study, can be attributed to the presence of plasmid-transferred mobilized colistin resistance mcr-1 gene [ 48 , 49 , 50 ]. Moreover, the colistin resistance was always related to resistance to tylosin, sulphamethoxazole, and amoxicillin, as recently reported [ 44 ].…”

Section: Discussionsupporting

confidence: 59%

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The Wide Range of Antibiotic Resistance and Variability of Genotypic Profiles in Escherichia coli from Domestic Animals in Eastern Sicily

et al. 2020

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The emergence of multidrug resistance among Enterobacteriaceae in livestock poses a serious public health threat. Escherichia coli, a usual host of intestinal microbiota, is recognized also as etiological agent of numerous infections widespread in both humans and animals. The colibacillosis is one of the most reported zoonoses worldwide, typically treated with antibiotics in the primary stages. This strategy has promoted the onset of antibiotic-resistant serotypes of E. coli, reducing the effectiveness of therapeutic treatments and contributing to antibiotic resistance spread. The current study focused on biodiversity, pathogenicity, and antibiotic resistance profile of 104 E. coli strains isolated from domestic animals in Eastern Sicily. The strains were isolated from sick animals and carcasses of six different animal species and screened for resistance against 16 antibiotic molecules, as recommended by WHO and OIE. The antibiotic resistance patterns highlighted that all strains were multi-resistant, showing resistance to at least three antibiotic classes. The highest incidence of resistance was observed against amoxicillin (100%), tylosin (97%), sulfamethoxazole (98%), and erythromycin (92%), while the lowest for colistin (8%). The pathotype characterization identified two EPEC strains and the study of genetic linkage (PFGE) showed a wide variety of profiles. The current study emphasized the wide range of multidrug resistance and genotyping profiles in E. coli isolated in Easter Sicily.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 76%

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The Wide Range of Antibiotic Resistance and Variability of Genotypic Profiles in Escherichia coli from Domestic Animals in Eastern Sicily

et al. 2020

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“…The mcr-1 gene is primarily found in E. coli and K. pneumoniae of human origin, which is in line with our study. The plasmid-mediated Col-R possibly developed in animals and was ultimately transmitted to humans [5,32,33]. The four human isolates which harboured mcr-1 in our study were isolated from one septic ICU patient and three patients with urinary tract infections from different wards.…”

Section: Discussionmentioning

confidence: 81%

Emergence of plasmid-mediated mcr genes from Gram-negative bacteria at the human-animal interface

Javed

Zafar²,

Ghafoor

et al. 2020

Gut Pathog

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Background The global emergence of plasmid-mediated colistin resistance (Col-R) conferred by mcr genes in gram-negative rods (GNRs) has jeopardized the last treatment option for multidrug-resistant bacterial infections in humans. This study aimed to assess the emergence of mcr gene-mediated Col-R in GNRs isolated from humans and animals in Pakistan. Methods Animal and clinical specimens collected from various sources were prospectively analysed using standard microbiological procedures. Pathogens were identified using the API 20E and API 20NE systems (bioMerieux). Minimum inhibitory concentration (MIC) against colistin was determined using the MIC detection methods, and multiplex polymerase chain reaction (PCR) was used to amplify the mcr-1 to mcr-5 genes. Results We isolated 126 (88.1%) animal and 17 (11.9%) human Col-R phenotypes, among which there was a significant association (P < 0.01) of Escherichia coli and Proteus mirabilis with animals and of Acinetobacter baumannii with humans. Animal strains exhibited statistically significant (P < 0.05) resistance to co-trimoxazole, chloramphenicol, and moxifloxacin, and the human pathogens exhibited statistically significant (P < 0.05) antibiotic resistance to cephalosporins, carbapenems, and piperacillin-tazobactam. For Col-R strains, MIC50 values were > 6 µg/mL and > 12 µg/mL for human and animal isolates, respectively. mcr genes were detected in 110 (76.9%) bacterial strains, of which 108 (98.2%) were mcr-1 and 2 (1.8%) were mcr-2. Conclusions The detection of a considerable number of mcr-1 and mcr-2 genes in animals is worrisome, as they are now being detected in clinical pathogens. The acquisition of mcr genes by colistin-susceptible bacteria could leave us in a post-antibiotic era.

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“…Colistin is a last resort for the treatment of infections caused by MDR gram-negative bacteria ( Johura et al., 2020 ), and in recent years, E. coli strains carrying the mcr-1 gene, which confers resistance to colistin, have become widespread in the poultry industry. In this study, the detection rate was very low; in fact, only 1 farm detected the mcr-1 gene at the early fattening stage.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal monitoring of multidrug resistance in Escherichia coli on broiler chicken fattening farms in Shandong, China

et al. 2021

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The extensive use of antibiotics has, in recent years, caused antimicrobial resistance and multidrug resistance in Escherichia coli to gradually develop into a worldwide problem. These resistant E. coli could be transmitted to humans through animal products and animal feces in the environment, thereby creating a problem for bacterial treatment for humans and animals and resulting in a public health issue. Monitoring the resistance of E. coli throughout the broiler fattening period is therefore of great significance for both the poultry industry and public health. In this longitudinal study, samples were taken from 6 conventional broiler fattening farms in Shandong Province, China, at 3 different times within 1 fattening period. The overall isolation rate of E. coli was 53.04% (375/707). Antibiotic resistance was very common in the E. coli isolated from these farms, and differed for different antibiotics, with ampicillin having the highest rate (92.86%) and cefoxitin the lowest (10.12%). Multidrug resistance was as high as 91.07%. More importantly, both the resistance rate of E. coli to the different drugs and the detection rate of drug resistance genes increased over time. The mobile colistin resistance ( mcr-1 ) gene was detected in 24.40% of the strains, and these strains often carried other drug resistance genes, such as those conferring aminoglycoside, β-lactamase, tetracycline, and sulfonamide resistance. Antimicrobial resistance and drug resistance genes in E. coli were least common in the early fattening stage. The individual detection rates of sul1 , sul3 , aacC4 , aphA3 , and mcr-1 were significantly lower ( P < 0.05) for the early fattening stage than for the middle and late stages. The rational use of antibiotics, in conjunction with the improvement of the breeding environment during the entire broiler fattening cycle, will be helpful in the development of the poultry industry and the protection of public health.

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Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh

Cited by 44 publications

References 32 publications

The Wide Range of Antibiotic Resistance and Variability of Genotypic Profiles in Escherichia coli from Domestic Animals in Eastern Sicily

The Wide Range of Antibiotic Resistance and Variability of Genotypic Profiles in Escherichia coli from Domestic Animals in Eastern Sicily

Emergence of plasmid-mediated mcr genes from Gram-negative bacteria at the human-animal interface

Longitudinal monitoring of multidrug resistance in Escherichia coli on broiler chicken fattening farms in Shandong, China

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