Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province

Zhang, X.; Zhang, B.; Guo, Yuqi; Wang, J.; Zhao, Pengfei; Liu, Juxiang; He, Kun

doi:10.1016/j.ijfoodmicro.2018.11.013

Cited by 37 publications

(38 citation statements)

References 21 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study demonstrated that the colistin resistance rate was maintained below 2% for three consecutive years after 2015. Indeed, the prevalence of colistin-resistant isolates in this study was consistent with previous reports in Poland [ 12 ] but lower than other reports from Japan (48%) [ 13 ], China (42%) [ 14 ], and Cambodia (20%) [ 1 ].…”

Section: Resultssupporting

confidence: 92%

See 1 more Smart Citation

Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea

et al. 2020

View full text Add to dashboard Cite

We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected mcr-3 genes in 17 isolates predominantly from diseased pigs. The mcr-3 genes were characterized as mcr-3.1 in 15 isolates and mcr-3.5 in 2 isolates. The mcr-3 gene was transferred to the E. coli J53 recipient strain from more than 50% of the mcr-3-carrying isolates. The mcr-3.1 and mcr-3.5 genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the mcr-3.5 encodes three amino acid substitutions compared with mcr-3.1. To the best of our knowledge, this is the first report of pathogenic E. coli carrying mcr-3.5 in South Korea, which implies that mcr-3 variants may have already been widely spread in the pig industry.

show abstract

Section: Resultssupporting

confidence: 92%

“…PCR analysis presented five replicon types (IncP1, HI2, I1-α, IncM, and IncN). The mcr - 3.1 gene was identified predominantly in the IncHI2 plasmid, which is associated with the spread of MDR, including β-lactams and quinolones [ 14 , 21 ]. Agreeing with Li et al [ 16 ], the mcr - 3.5 genes belonged to IncP plasmid.…”

Section: Resultsmentioning

confidence: 99%

Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea

et al. 2020

View full text Add to dashboard Cite

show abstract

“…At present, there is social pressure on the poultry production system that demands the prohibition of antibiotic administration during the growing period. It has been demonstrated that stringent programs for AMR control result in lower AMR rates in poultry production ( Miflin et al., 2007 ; Zhang et al., 2019 ). Thus, the poultry sector is making a significant effort to eliminate antibiotic administration as much as possible from growing farms.…”

Section: Discussionmentioning

confidence: 99%

Genotyping and molecular characterization of antimicrobial resistance in thermophilic Campylobacter isolated from poultry breeders and their progeny in Eastern Spain

et al. 2020

View full text Add to dashboard Cite

Thermophilic Campylobacter spp. are recognized as a major cause of acute bacterial diarrhea in humans, with broiler meat being the most common source of human infection. Antibiotic therapy is usually necessary for severe or prolonged infections, especially in immunocompromised populations such as young or elderly individuals. However, different studies have demonstrated a close association between antibiotic use in animal production and antimicrobial resistance ( AMR ) in humans. In this sense, there is social pressure to reduce antibiotic administration and find adequate alternatives to control the presence of bacterial infections in farms. However, there is a lack of information related to Campylobacter AMR dynamics through the entire production system from breeders to their progeny. It is unknown if resistance genes are a result of adaptation through chromosomal mutation or through horizontal gene transfer, instead of vertical transmission of DNA from the parent to their progeny. Thus, the main objectives of this study were to assess the main AMR rates present in a poultry production system, to study the relationship between Campylobacter AMR profiles from breeders and their progeny, and to study the presence and distribution of antibiotic resistance genes in poultry production. Regarding AMR rates, ciprofloxacin was classified as extremely high, followed by nalidixic acid and tetracyclines that were classified as very high. Moreover, this study demonstrated a relationship between the AMR patterns and genes found from Campylobacter strains isolated in breeders and those present in their progeny.

show abstract

“…The first report of mcr-1 in a clinical human isolate from Egypt and Africa was in (Elnahriry et al, 2016 following the first reports in animals in Algeria (Olaitan et al, 2016) and Egypt (Khalifa et al, 2016). The high prevalence of mcr-1 in animal isolates compared to human clinical isolates is a strong proof that the antimicrobials misuse in livestock is probably increasing the risk of zoonotic transmission of these bacteria from animals to humans (Zhao et al, 2018).…”

Section: Advances In Animal and Veterinary Sciences December 2019 | Vmentioning

confidence: 99%

Virulence Gene Constellations Associated with Lethality in Avian Pathogenic E. coli Recovered from Broiler Chickens

Ali¹,

El-Mawgoud²,

Dahshan³

et al. 2019

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

In this study, avian pathogenic E. coli (APEC) isolated from broiler chickens in Fayoum and Beni-Suef governorates in Egypt were morphologically and biochemically identified. Selected isolates were serologically identified, and their antimicrobial susceptibility profiles were determined. Ten isolates from the most predominant serogroups were subjected to virulence and antimicrobial resistance genes detection using PCR. In vivo pathogenicity test was performed for 5 E. coli strains that were selected based on serogroups prevalence, virulence, and antimicrobial genes patterns. Results revealed an incidence of 53.8% of E. coli from broiler chickens with the predominance of O 125 (30%), O 119 (20%), O 126 , and O 86a (15% each) serogroups. The selected 20 E. coli strains were multi-drug resistant (MDR) but sensitive to fosfomycin. The β-lactams and tetracyclines resistance genes were detected in all tested isolates, however, aminoglycoside and quinolone resistance genes were not detected. In this study, 10% of isolates were resistant to colistin and the mcr-1 gene was detected by PCR. In vivo pathogenic strains consistently harbored the virulence gene pattern of fimH, fimA, papC, iutA, and tsh which was the most common gene constellation detected. The detection of tsh gene was consistently associated with lethality in day-old chicks. In conclusion, the current study demonstrated the high prevalence of multidrug-resistant E. coli in broiler chickens representing a potential public health concern. Additionally, the virulence gene constellation of fimH, fimA, papC, iutA, and tsh was found to be associated with lethality in day-old chicks and can be used as a virulence marker of APEC.

show abstract

Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province

Cited by 37 publications

References 21 publications

Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea

Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea

Genotyping and molecular characterization of antimicrobial resistance in thermophilic Campylobacter isolated from poultry breeders and their progeny in Eastern Spain

Virulence Gene Constellations Associated with Lethality in Avian Pathogenic E. coli Recovered from Broiler Chickens

Contact Info

Product

Resources

About