Colicins and other Bacteriocins with Established Modes of Action

Konisky, J

doi:10.1146/annurev.mi.36.100182.001013

Cited by 374 publications

(221 citation statements)

References 53 publications

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“…Finally, BlyB could function enzymatically to alter BlyA post-translationally, a step that could be needed for full activation of haemolytic activity. Accessory proteins that fulfil these sorts of functions have been well documented for other haemolysins and cytolytic activities, such as colicins and bacteriocins (Konisky, 1982;Issartel et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Cloning and analysis of a Borrelia burgdorferi membrane‐interactive protein exhibiting haemolytic activity

Guina

Oliver

1997

Molecular Microbiology

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SummaryWe cloned the gene encoding a membrane-interactive protein of Borrelia burgdorferi by means of its haemolytic activity in Escherichia coli. The haemolytic activity was erythrocyte-species specific, with progressively decreasing activity for erythrocytes from horse, sheep, and rabbit, respectively. Genetic analysis of the haemolytic determinant revealed two borrelia haemolysin genes, blyA and blyB, that are part of a predicted four-gene operon which is present in multiple copies on the 30 kb circular plasmid(s) of B. burgdorferi B31. blyA encodes a predicted ␣-helical 7.4 kDa protein with a hydrophobic central region and a positively charged C-terminus, which is structurally homologous to a large group of pore-forming toxins with cytolytic activity. blyB encodes a soluble protein which stabilized BlyA and enhanced haemolytic activity. While the majority of BlyA in E. coli was membrane-associated, only soluble protein was haemolytically active. The haemolytic activity was shown to be highly protease sensitive, heat labile, independent of divalent cations, and extremely dependent on protein concentration, consistent with a requirement for oligomerization as the mechanism of action. BlyA was highly purified from E. coli in a single step utilizing Triton X-114 phase partitioning. Genetic analysis of blyA and blyB mutants indicated that the stability, membrane association, and activity of BlyA was dependent on subtle changes in its sequence and on the BlyB protein. The bly genes were found to be expressed at a very low level in cultured B. burgdorferi.

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Section: Discussionmentioning

confidence: 99%

Cloning and analysis of a Borrelia burgdorferi membrane‐interactive protein exhibiting haemolytic activity

Guina

Oliver

1997

Molecular Microbiology

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“…Since the bacteriocin colicin was identified from Escherichia coli in 1920s, numerous microorganisms have been screened for their production of bacteriocins (Konisky 1982). In the past 20 years, there have been many studies focused on those produced by G + bacteria, particularly by LAB.…”

Section: Bacteriocins From Labmentioning

confidence: 99%

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

Wan

Saris

et al. 2012

Appl Microbiol Biotechnol

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“…Many bacteria, such as Escherichia Coli and related bacteria, carry colicin systems [7,17]. Colicin systems are gene complexes that code for a toxic protein, i.e.…”

Section: Introductionmentioning

confidence: 99%

Optimization as Side-Effect of Evolving Allelopathic Diversity

Pagie

Hogeweg

2000

Parallel Problem Solving From Nature PPSN VI

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Abstract. Many bacteria carry gene complexes that code for a toxin-antidote pair, e.g. colicin systems. Such gene complexes can be advantageous for its host by killing competitor bacteria while the antidote protects the host. However, in order to evolve a novel and useful toxin first a proper antidote must be evolved. We present a model of bacteria that can express and evolve such allelopathic systems. Although in the model novel types must evolve from existing types we find that nevertheless in general a high diversity of toxins evolves and, as a sideeffect thereof, generalized immunity mechanisms. We interpret the allelopathic systems in terms of an optimization problem: fitness cases are toxins and solutions present (potential) antidotes. As a side-effect of the evolution of allelopathic systems generalized solutions of the optimization task are evolved as well.

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Colicins and other Bacteriocins with Established Modes of Action

Cited by 374 publications

References 53 publications

Cloning and analysis of a Borrelia burgdorferi membrane‐interactive protein exhibiting haemolytic activity

Cloning and analysis of a Borrelia burgdorferi membrane‐interactive protein exhibiting haemolytic activity

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

Optimization as Side-Effect of Evolving Allelopathic Diversity

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