Colicins and Microcins Produced by Enterobacteriaceae: Characterization, Mode of Action, and Putative Applications

Marković, Katarina G.; Grujović, Mirjana Ž.; Koraćević, Maja G.; Nikodijević, Danijela D.; Milutinović, Milena; Semedo-Lemsaddek, Teresa; Djilas, Milan

doi:10.3390/ijerph191811825

Cited by 28 publications

(27 citation statements)

References 151 publications

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“…strains harbored a tetA gene responsible for tetracycline resistance and caa gene that codes for colicin polypeptide toxins known to destroy cells of other organisms by depolarizing the cytoplasmic membrane of the cells (45,46). Also, the plasmid in the K. quasivariicola harbored the cusC gene associated with copper and silver resistance, which, when found in pathogenic K.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterisation of Multidrug-Resistant Pathogenic Enteric Bacteria from healthy children in Osun State, Nigeria

Uwanibe

Olawoye

Happi

et al. 2023

Preprint

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Antimicrobial resistance (AMR) has been established to be a significant driver for the persistence and spread of bacterial infections. It is, therefore, essential to conduct epidemiological surveillance of AMR in healthy individuals to understand the actual dynamics of AMR in Nigeria. Multi-drug resistant Klebsiella quasivariicola (n=1), Enterobacter hormaechei (n=1), and Escherichia coli (n=3) from stool samples of healthy children were subjected to whole genome sequencing using Illumina Nextseq1000/2000 and Oxford nanopore. Bioinformatics analysis reveals antimicrobial resistance, virulence genes, and plasmids. This pathogenic enteric bacteria harbored more than three plasmid replicons of either Col and/or Inc type associated with outbreaks and AMR resistant gene pmrB responsible for colistin resistance. Plasmid reconstruction revealed an integrated tetA gene responsible for tetracycline resistance, and caa gene responsible for toxin production in two of the E.coli isolates, and a cusC gene known to induce neonatal meningitis in the K. quasivariicola ST3879. The global spread of MDR pathogenic enteric bacteria is a worrying phenomenon, and close surveillance of healthy individuals, especially children, is strongly recommended to prevent the continuous spread and achieve the elimination and eradication of these infections. Molecular epidemiological surveillance using whole genome sequencing (WGS) will improve the detection of MDR pathogens in Nigeria.

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Section: Discussionmentioning

confidence: 99%

Genomic Characterisation of Multidrug-Resistant Pathogenic Enteric Bacteria from healthy children in Osun State, Nigeria

Uwanibe

Olawoye

Happi

et al. 2023

Preprint

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“…88 Colicins and microcins can degrade the peptidoglycan layer and phosphatase activity as well as RNAase and DNAase activity. 89 ■ GENERAL CHARACTERISTICS OF AMPS Most AMPs have certain similar features, which are the inclusion of both ionic and hydrophobic amino acids, giving a characteristic amphiphilic nature to AMPs (Table 1). 102 Given that bacterial membrane has an overall negative charge, the inclusion of cationic amino acids such as arginine and lysine greatly enhances the antibacterial property of these peptides, as it helps these peptides to electrostatically bind to the bacterial membrane.…”

Section: ■ Development Of Resistance In Bacteriamentioning

confidence: 99%

“…Bacteriocins disrupt DNA structure as well as the permeability and integrity of the cell membrane, causing leakage of intracellular biomolecules to render cells abnormally distorted . Colicins and microcins can degrade the peptidoglycan layer and phosphatase activity as well as RNAase and DNAase activity …”

Section: Host Defense Peptidesmentioning

confidence: 99%

Peptide Dendrimer-Based Antibacterial Agents: Synthesis and Applications

Paul,

Verma,

Chen

2024

ACS Infect. Dis.

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Pathogenic bacteria cause the deaths of millions of people every year. With the development of antibiotics, hundreds and thousands of people’s lives have been saved. Nevertheless, bacteria can develop resistance to antibiotics, rendering them insensitive to antibiotics over time. Peptides containing specific amino acids can be used as antibacterial agents; however, they can be easily degraded by proteases in vivo. To address these issues, branched peptide dendrimers are now being considered as good antibacterial agents due to their high efficacy, resistance to protease degradation, and low cytotoxicity. The ease with which peptide dendrimers can be synthesized and modified makes them accessible for use in various biological and nonbiological fields. That is, peptide dendrimers hold a promising future as antibacterial agents with prolonged efficacy without bacterial resistance development. Their in vivo stability and multivalence allow them to effectively target multi-drug-resistant strains and prevent biofilm formation. Thus, it is interesting to have an overview of the development and applications of peptide dendrimers in antibacterial research, including the possibility of employing machine learning approaches for the design of AMPs and dendrimers. This review summarizes the synthesis and applications of peptide dendrimers as antibacterial agents. The challenges and perspectives of using peptide dendrimers as the antibacterial agents are also discussed.

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“…Recently, researchers have exhibited signi cant interest in the potential use of microcins for cancer treatment because of their selective nature, which results in higher e cacy and minimal adverse effects. [9,10]. MccJ25 is a 21-amino acid peptide produced by some Enterobacterales, especially E. coli, and has a stable structure to avoid degradation by proteases in the digestive tract [8].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Antimicrobial Peptide Microcin J25 (MccJ25) Isolated from Escherichia coli against Breast Cancer Cells

Shalibeik,

Ghandehari,

Ahadi

et al. 2024

Preprint

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Introduction: Microcins are Antimicrobial peptides (AMPs) with low molecular weight, which are produced by Enterobacterales and have broad-spectrum antibacterial activity. They can selectively replace common cancer treatments in cancer cells with less side effects and higher effectiveness. Given the aforementioned context, the present study endeavors to examine the antitumor activity of microcins isolated from of the Enterobacterales. Material and Methods In total, 120 Enterobacterales isolates were examined after identification. Subsequently, the bacteria were subjected to an agar diffusion test to assess their antibacterial efficacy. Positive isolates were further examined for the presence of Mccj25 using PCR. The cytotoxic effects of isolates harboring the microcin gene were explored using quantitative real-time PCR (RT-qPCR) and the MTT test on breast cancer cells. Additionally, the expression levels of BCL2 and STAT3 genes were evaluated, and apoptosis was quantified using flow cytometry. The repair rate of normal cells was determined using a scratch assay. Results The findings obtained from the phenotypic and biochemical assays have duly verified and established the categorization of the Enterobacterales. After conducting the agar diffusion test, a total of 25 isolates of Escherichia coli and Klebsiella pneumoniae displaying inhibition zones were chosen as suitable specimens possessing AMPs. Urinary E. coli was identified as isolate 83. The analysis conducted on the expression of the Mccj25 gene within the aforementioned isolates indicated that isolate 83 exhibited significant expression of the Mccj25 gene. Conclusion The extract obtained from this isolate on the breast cancer cell line exhibited the most significant degree of toxicity after precisely 48 h. Furthermore, the treatment of breast cancer cells with isolate 83 showed that the rate of apoptosis was about 86%, and the expression of BCL2 and STAT3 genes decreased. Moreover, it potentiated the reparative ability of normal fibroblast cells. They resulted in growth suppression of breast cancer cells and elicited an escalated rate of cellular demise via the apoptosis pathway.

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Colicins and Microcins Produced by Enterobacteriaceae: Characterization, Mode of Action, and Putative Applications

Cited by 28 publications

References 151 publications

Genomic Characterisation of Multidrug-Resistant Pathogenic Enteric Bacteria from healthy children in Osun State, Nigeria

Genomic Characterisation of Multidrug-Resistant Pathogenic Enteric Bacteria from healthy children in Osun State, Nigeria

Peptide Dendrimer-Based Antibacterial Agents: Synthesis and Applications

Protective Effects of Antimicrobial Peptide Microcin J25 (MccJ25) Isolated from Escherichia coli against Breast Cancer Cells

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