Cold tolerance of third-instar Drosophila suzukii larvae

Jakobs, Ruth; Ahmadi, Banafsheh; Houben, Sarah; Gariépy, Tara D.; Sinclair, Brent J.

doi:10.1016/j.jinsphys.2016.10.008

Cited by 21 publications

(14 citation statements)

References 32 publications

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“…This apparent discrepancy may be a consequence of variation in the indices applied to the individual cold tolerance assays, i.e. chill coma, supercooling point, lethal time, lethal temperature etc., [53][54][55][56] and/or variance in experimental factors, e.g. the age of the flies or the temperature at which they are tested.…”

Section: Discussionmentioning

confidence: 99%

Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii

Terhzaz

Alford

Yeoh

et al. 2017

Pest Management Science

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BACKGROUNDNeuropeptides are central to the regulation of physiological and behavioural processes in insects, directly impacting cold and desiccation survival. However, little is known about the control mechanisms governing these responses in Drosophila suzukii. The close phylogenetic relationship of D. suzukii with Drosophila melanogaster allows, through genomic and functional studies, an insight into the mechanisms directing stress tolerance in D. suzukii.RESULTSCapability (Capa), leucokinin (LK), diuretic hormone 44 (DH44) and DH31 neuropeptides demonstrated a high level of conservation between D. suzukii and D. melanogaster with respect to peptide sequences, neuronal expression, receptor localisation, and diuretic function in the Malpighian tubules. Despite D. suzukii's ability to populate cold environments, it proved sensitive to both cold and desiccation. Furthermore, in D. suzukii, Capa acts as a desiccation‐ and cold stress‐responsive gene, while DH 44 gene expression is increased only after desiccation exposure, and the LK gene after nonlethal cold stress recovery.CONCLUSIONThis study provides a comparative investigation into stress tolerance mediation by neuroendocrine signalling in two Drosophila species, providing evidence that similar signalling pathways control fluid secretion in the Malpighian tubules. Identifying processes governing specific environmental stresses affecting D. suzukii could lead to the development of targeted integrated management strategies to control insect pest populations. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii

Terhzaz

Alford

Yeoh

et al. 2017

Pest Management Science

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“…In our study, no accumulation of cryoprotectants and consequently no cold hardiness were determined in RCH regime. Jakobs et al (2017) found no evidence that acute cold tolerance of D. suzukii larvae could be improved by RCH.…”

Section: Discussionmentioning

confidence: 86%

Cold acclimation ofTrogoderma granariumEverts is tightly linked to regulation of enzyme activity, energy content and ion concentration

Mohammadzadeh

Izadi

2018

Preprint

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In this study, cold hardiness and some physiological characteristics of T. granarium larvae were investigated under different thermal regimes, i.e. warm-acclimated (WA), cold-acclimated (CA), fluctuating-acclimated (FA) and rapid cold-hardened (RCH). In all regimes, the survival rate of the larvae decreased with a decrease in temperature and raise in exposure time. Cold acclimated larvae showed the highest cold hardiness in -15 and -20 ºC. Control larvae had the highest glycogen content (34.4 ± 2.3 µg/gdw). In contrast, cold acclimation larvae had the lowest glycogen content (23.0 ± 1.6 µg/gdw). Change in trehalose content was reversely proportional to change in glycogen content. The greatest myo-inositol and glucose contents were detected in larvae cold acclimation treatment (10.7 ± 0.4 µg/gdw) and control (0.49 ± 0.03 µg/gdw), respectively. In control and treated larvae, the concentration of Na+ decreased, though the concentration of K+ rose, with rising the exposure time. The shape of the thermal reaction of AMP-depended protein kinase and protein phosphatase IIC followed the same norm, which is different from protein phosphatase I and protein phosphatase IIA. Protein phosphatase IIA and IIC showed a complete difference in thermal reaction norms. In did, thermal fluctuation caused the highest changes in the activity of the enzymes, whereas the RCH showed the lowest changes in the activity of the enzyme. Our results showed a significant enhancement of larval cold tolerance under CA regime that is related to the level of low molecular weight carbohydrates, protein kinase, and phosphatases activity, and hemolymph ions concentration.SUMMARY STATEMENTIn Trogoderma granarium, cold acclimation enhances the larval cold tolerance that is related to change in the level of low molecular weight carbohydrates, protein kinase, and phosphatases activity, and hemolymph ions concentration.

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Section: Discussionmentioning

confidence: 91%

Cold Acclimation of Trogoderma granarium Everts Is Tightly Linked to Regulation of Enzyme Activity, Energy Content, and Ion Concentration

Mohammadzadeh

Izadi

2018

Front. Physiol.

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In this study, cold hardiness and some physiological characteristics of the Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) larvae, were investigated under different thermal regimes, i.e., control, cold-acclimated (CA), fluctuating-acclimated (FA), and rapid cold-hardened (RCH). In all the regimes, the larval survival rate decreased with a decrease in temperature. CA larvae showed the highest cold hardiness following 24 h exposure at -15 and -20°C. Control larvae had the highest glycogen content (34.4 ± 2.3 μg/dry weight). In contrast, CA larvae had the lowest glycogen content (23.0 ± 1.6 μg/dry weight). Change in trehalose content was reversely proportional to changes in glycogen content. The highest myo-inositol and glucose contents were detected in CA larvae (10.7 ± 0.4 μg/dry weight) and control (0.49 ± 0.03 μg/dry weight), respectively. In control and treated larvae, [Na+] decreased, though [K+] increased, with increasing exposure time. The shape of the thermal reaction curve of AMP-depended protein kinase and protein phosphatase 2C followed the same norm, which was different from protein phosphatase 1 and protein phosphatase 2A. Protein phosphatase 2A and 2C showed a complete difference in thermal reaction norms. Indeed, thermal fluctuation caused the highest changes in the activity of the enzymes, whereas the RCH showed the lowest changes in the activity of the enzymes. Our results showed a significant enhancement of larval cold tolerance under CA regime, which is related to the high levels of low molecular weight carbohydrates under this regime. Our results showed that among the different thermal regimes tested, the CA larvae had the lowest supercooling point (about -22°C) and the highest cold hardiness following 24 h exposure at -15 and -20°C.

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Cold tolerance of third-instar Drosophila suzukii larvae

Cited by 21 publications

References 32 publications

Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii

Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii

Cold acclimation ofTrogoderma granariumEverts is tightly linked to regulation of enzyme activity, energy content and ion concentration

Cold Acclimation of Trogoderma granarium Everts Is Tightly Linked to Regulation of Enzyme Activity, Energy Content, and Ion Concentration

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