Cold Tolerance of an Inbred Line Population of Rice (Oryza sativa L) at Different Growth Stages

Ranawake, A. L.; Nakamura, Chiharu

doi:10.4038/tare.v14i2.4838

Cited by 8 publications

(10 citation statements)

References 42 publications

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“…Bioassay conditions for CTG and CTS after the first cold stress were according to our previous report. [ 36 ] Briefly, for CTG, seeds of HGKN and HOK were exposed to 50 °C for five days to break dormancy and surface sterilized by dipping in 70% (v/v) ethanol for 1 min and in 1% (w/v) solution of NaOCl for 1 h followed by washing three times in sterilized distilled water. Imbibed and surface-sterilized seeds were then placed in wet Petri dishes and incubated under continuous cold stress at 15 °C in dark after pre-incubation for two days at 20 °C.…”

Section: Methodsmentioning

confidence: 99%

“…We previously developed experimental protocols suitable for evaluating CTG and CTS, using a cold tolerant japonica cultivar ‘Hyogo-Kitanishiki’ (abbreviated as HGKN) and a cold sensitive indica cultivar ‘Hokuriku-142’. [ 36 ] Based on the protocols, we studied CTG and CTS of the RILs derived from this japonica × indica cross. In this study, we performed QTL analysis for CTG and CTS, using the previous bioassay data.…”

Section: Introductionmentioning

confidence: 99%

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Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativaL.)

Ranawake

Manangkil

Yoshida

et al. 2014

Biotechnology & Biotechnological Equipment

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Cold tolerance is an important breeding target in rice production. We studied quantitative trait loci (QTLs) controlling cold tolerance at germination (CTG) and early seedling (CTS) stages, using recombinant inbred lines derived from a japonica × indica cross. CTG was evaluated based on the percentage rate of germination at 15 °C for 12 days after pre-incubation of imbibed seeds at 20 °C for 2 days. For CTS, seven-day-old seedlings grown at 25 °C were subjected to two consecutive periods of three-day cold stress at 4 °C with an intervening eight-day recovery at 25 °C. CTS evaluation was according to an arbitrary five-point rating system at the fifth day of recovery after each stress period. No correlations were found between CTG and CTS, while a weak correlation was detected between CTS after the first and second stress. By the composite interval mapping, five QTLs for CTG explaining 5.7%–9.3% of the total phenotypic variance (PVE) and nine for CTS with PVE of 5.8%–35.6% were detected. Only one of these QTLs was common, indicating growth-stage specificity of cold tolerance. Four of the five QTLs after the second cold stress were different from the ones after the first cold stress. Analysis of variance test showed significant interactions between alleles at the QTL sites and the two stress conditions with respect to the mean CTS scores. A possible involvement of cold acclimation and usefulness of japonica germplasms in breeding for cold tolerance in indica rice was discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativaL.)

Ranawake

Manangkil

Yoshida

et al. 2014

Biotechnology & Biotechnological Equipment

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“…However, tolerance of either cold or heat stress at the vegetative stage does not automatically translate into tolerance at the reproductive stage (Ranawake and Nakamura, 2011). However, tolerance of either cold or heat stress at the vegetative stage does not automatically translate into tolerance at the reproductive stage (Ranawake and Nakamura, 2011).…”

Section: Vegetative Growthmentioning

confidence: 99%

“…On the other extreme, cold-temperature stress adversely affects rice at the seedling stage in the subtropics (Ranawake and Nakamura, 2011) and at all growth stages in the temperate regions where it is grown as a summer crop. The critical threshold below which rice plants start to show a negative response is documented to be ≤15°C (Zhang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Plant Abiotic Stress: Temperature Extremes

Bahuguna

Jagadish

Coast

et al. 2014

Encyclopedia of Agriculture and Food Systems

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“…The three rice japonica cultivars (Hayayuki, Spring, and 3011) had mean shoot lengths of 105, 98, and 41mm, respectively, when planted at normal depth (1.5cm) under normal temperature (25°C) for 14d ( Table 3 ). ‘Hayayuki’ from Japan [ 43 ], has been used as cold-tolerant reference in other experiments and so was included here together with the more recently commercialized cold-tolerant, tropical japonica ‘Spring’ from Arkansas and another putative cold-tolerant japonica cultivar from Chile ‘3011’. The 10 weedy rice accessions tested had shoot lengths ranging from 55 to 191mm, with PRA08-D-BH as the fastest growing ecotype under normal temperature.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Stress-Responsive Gene Expression in Cultivated and Weedy Rice Differing in Cold Stress Tolerance

et al. 2015

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Rice (Oryza sativa L.) cultivars show impairment of growth in response to environmental stresses such as cold at the early seedling stage. Locally adapted weedy rice is able to survive under adverse environmental conditions, and can emerge in fields from greater soil depth. Cold-tolerant weedy rice can be a good genetic source for developing cold-tolerant, weed-competitive rice cultivars. An in-depth analysis is presented here of diverse indica and japonica rice genotypes, mostly weedy rice, for cold stress response to provide an understanding of different stress adaptive mechanisms towards improvement of the rice crop performance in the field. We have tested a collection of weedy rice genotypes to: 1) classify the subspecies (ssp.) grouping (japonica or indica) of 21 accessions; 2) evaluate their sensitivity to cold stress; and 3) analyze the expression of stress-responsive genes under cold stress and a combination of cold and depth stress. Seeds were germinated at 25°C at 1.5- and 10-cm sowing depth for 10d. Seedlings were then exposed to cold stress at 10°C for 6, 24 and 96h, and the expression of cold-, anoxia-, and submergence-inducible genes was analyzed. Control plants were seeded at 1.5cm depth and kept at 25°C. The analysis revealed that cold stress signaling in indica genotypes is more complex than that of japonica as it operates via both the CBF-dependent and CBF-independent pathways, implicated through induction of transcription factors including OsNAC2, OsMYB46 and OsF-BOX28. When plants were exposed to cold + sowing depth stress, a complex signaling network was induced that involved cross talk between stresses mediated by CBF-dependent and CBF-independent pathways to circumvent the detrimental effects of stresses. The experiments revealed the importance of the CBF regulon for tolerance to both stresses in japonica and indica ssp. The mechanisms for cold tolerance differed among weedy indica genotypes and also between weedy indica and cultivated japonica ssp. as indicated by the up/downregulation of various stress-responsive pathways identified from gene expression analysis. The cold-stress response is described in relation to the stress signaling pathways, showing complex adaptive mechanisms in different genotypes.

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Cold Tolerance of an Inbred Line Population of Rice (Oryza sativa L) at Different Growth Stages

Cited by 8 publications

References 42 publications

Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativaL.)

Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativaL.)

Plant Abiotic Stress: Temperature Extremes

Analysis of Stress-Responsive Gene Expression in Cultivated and Weedy Rice Differing in Cold Stress Tolerance

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