Cold tolerance and supercooling points of two ladybird beetles (Col.: Coccinellidae): Impact of the diet

Pourani, Maryam Salehi; Mahdian, Kamran; Izadi, Hamzeh; Basirat, Mehdi; Sahhafi, Seyyed Rasoul

doi:10.1016/j.cryobiol.2019.10.197

Cited by 5 publications

(4 citation statements)

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“…In such a way that LT 50 is larger than SCP, the insect is chill susceptible (LT 50 > SCP = chill susceptible) ( Sinclair et al, 2015 ). Our previous study ( Pourani et al, 2019 ) showed that in Cheilomenes sexmaculata (F.) and Oenopia conglobata contaminata (Menetries) (Col.: Coccinellidae), the highest mortality occurred above the SCP. So, these ladybird beetles are also considered chill-intolerant species.…”

Section: Discussionmentioning

confidence: 99%

“…The temperature was gradually reduced from 25 °C to the desired temperatures (−10 °C to 0 °C) at a rate of 0.5 °C per minute. The insects were kept at each temperature for 24 h. Subsequently, the temperature was slowly raised (0.5 °C per minute) back to 25 °C (the optimal growth temperature), and the survival of the insects was assessed after 24 h. Insects that exhibited no movement in their appendages were classified as deceased (Pourani et al, 2019). The experiment was conducted for 12 months, with three replications per month, and each replication included five adult insects.…”

Section: Cold Tolerance Assaymentioning

confidence: 99%

“…In addition to diapause initiation, cold hardiness is also necessary to ensure the insect’s life. Cold hardiness, also known as cold tolerance, refers to an insect species’ ability to prevent cold injuries and survive exposure to harsh winter conditions ( Andreadis and Athanassiou, 2017 ; Pourani et al, 2019 ). In most insects, there are typically two primary ways to acquire this ability: First, by maintaining a supercooled state through the synthesis and accumulation of cryoprotectants.…”

Section: Introductionmentioning

confidence: 99%

“…In most insects, there are typically two primary ways to acquire this ability: First, by maintaining a supercooled state through the synthesis and accumulation of cryoprotectants. And second, by expanding the supercooling capacity without accumulating cryoprotectants ( Storey et al, 1991 ; Bemani et al, 2012 ; Heydari and Izadi, 2014 ; Sinclair et al, 2015 ; Vrba et al, 2017 ; Pourani et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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The supercooling point depression is the leading cold tolerance strategy for the variegated ladybug, [Hippodamia variegata (Goezel)]

Khabir,

Izadi,

Mahdian

2023

Front. Physiol.

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The variegated ladybug, Hippodamia variegata is one of the most effective predators of various pests that hibernate as adult beetles. During the overwintering period from April 2021 to March 2022, we examined the supercooling point (SCP), cold tolerance, and physiological adaptations of beetles in Kerman, Iran. The beetles exhibited their greatest cold tolerance (63.4% after 24 h at −5°C) when their SCP was lowest (−23.2°C). Conversely, from April to October 2021, the SCP reached its peak (approximately −13.0°C), while cold tolerance was at its lowest level (6.7% after 24 h at −5°C). Cryoprotectant content (trehalose, glycerol, and glucose) was at its highest level in September (11.15, 10.82, and 6.31 mg/g, respectively). The critical thermal minimum (CTmin) reached its lowest point of −2.2°C in January and February. The lowest point of the lower lethal temperature (LLT) coincided with the lowest level of the SCP and the highest level of cold tolerance (in February, LT50 = −5.3°C, SCP = −23.2°C, and survival = 77.78% at −4°C/24 h). Chill-coma recovery time (CCRT) was examined at five different temperatures and two different exposure durations. The CCRT increased with a decrease in exposure temperature and time (68.0 s at −2°C after 2 h and 102.0 s at −2°C after 4 h). As the majority of the overwintering beetle’s mortality occurred at temperatures significantly higher than SCP, the adults of H. variegata are chill-susceptible insects that primarily rely on a depressed supercooling point to cope with unfavorable conditions during the overwintering period.

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Section: Discussionmentioning

confidence: 99%

Section: Cold Tolerance Assaymentioning

confidence: 99%