Cold-stored platelet hemostatic capacity is maintained for three weeks of storage and associated with taurine metabolism

Shea, Susan M.; Reisz, Julie A.; Mihalko, Emily P.; Rahn, Katelin C.; Rassam, Rassam M.G.; Chitrakar, Alisha; Gamboni, Fabia; D’Alessandro, Angelo; Spinella, Philip C.; Thomas, Kimberly A.

doi:10.1016/j.jtha.2023.11.025

Cited by 4 publications

(1 citation statement)

References 51 publications

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“…As a result, it is thought that cold-stored platelets can improve upon those disadvantages found with room temperature stored platelets [62]. This is despite initial reports that indicated that cold-stored platelets had a more rapid clearance from circulation and lower response to agonists [63,64] but this may not be the case since those phenotypic changes may have been overestimated and are partly due to impaired taurine and purine metabolism [65]. This is the driving force behind the development of systems that improve platelet longevity and increased research into cold-storage of platelet concentrates or freezing of units after collection for use at times of low platelet product inventories [66].…”

Section: Alternatives To Room Temperature Storagementioning

confidence: 99%

Bacterial Contamination of Platelet Products

Jacobs,

Zhou,

Tayal

et al. 2024

Microorganisms

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Transfusion of bacterially contaminated platelets, although rare, is still a major cause of mortality and morbidity despite the introduction of many methods to limit this over the past 20 years. The methods used include improved donor skin disinfection, diversion of the first part of donations, use of apheresis platelet units rather than whole-blood derived pools, primary and secondary testing by culture or rapid test, and use of pathogen reduction. Primary culture has been in use the US since 2004, using culture 24 h after collection of volumes of 4–8 mL from apheresis collections and whole-blood derived pools inoculated into aerobic culture bottles, with limited use of secondary testing by culture or rapid test to extend shelf-life from 5 to 7 days. Primary culture was introduced in the UK in 2011 using a “large-volume, delayed sampling” (LVDS) protocol requiring culture 36–48 h after collection of volumes of 16 mL from split apheresis units and whole-blood derived pools, inoculated into aerobic and anaerobic culture bottles (8 mL each), with a shelf-life of 7 days. Pathogen reduction using amotosalen has been in use in Europe since 2002, and was approved for use in the US in 2014. In the US, recent FDA guidance, effective October 2021, recommended several strategies to limit bacterial contamination of platelet products, including pathogen reduction, variants of the UK LVDS method and several two-step strategies, with shelf-life ranging from 3 to 7 days. The issues associated with bacterial contamination and these strategies are discussed in this review.

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Section: Alternatives To Room Temperature Storagementioning

confidence: 99%

Bacterial Contamination of Platelet Products

Jacobs,

Zhou,

Tayal

et al. 2024

Microorganisms

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Early Cold Stored Platelet Transfusion Following Severe Injury

Sperry,

Guyette,

Rosario-Rivera

et al. 2024

Annals of Surgery

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Objective: To determine the feasibility, efficacy, and safety of early cold stored platelet transfusion compared to standard care resuscitation in patients with hemorrhagic shock. Summary Background Data: Data demonstrating the safety and efficacy of early cold stored platelet transfusion are lacking following severe injury. Methods: A phase 2, multicenter, randomized, open label, clinical trial was performed at five U.S. trauma centers. Injured patients at risk of large volume blood transfusion and the need for hemorrhage control procedures were enrolled and randomized. The intervention was the early transfusion of a single apheresis cold stored platelet unit, stored for up to 14 days vs. standard care resuscitation. The primary outcome was feasibility and the principal clinical outcome for efficacy and safety was 24-hour mortality. Results: Mortality at 24 hours was 5.9% in patients who were randomized to early cold stored platelet transfusion compared to 10.2% in the standard care arm (difference, -4.3%; 95% CI, -12.8% to 3.5%; P=0.26). No significant differences were found for any of the prespecified ancillary outcomes. Rates of arterial and/or venous thromboembolism and adverse events did not differ across treatment groups. Conclusions and Relevance: In severely injured patients, early cold stored platelet transfusion is feasible, safe and did not result in a significant lower rate of 24-hour mortality. Early cold stored platelet transfusion did not result in a higher incidence of arterial and/or venous thrombotic complications or adverse events. The storage age of the cold stored platelet product was not associated with significant outcome differences.

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Lipidomic changes occurring in platelets during extended cold storage

Green,

Padula,

Dodgen

et al. 2024

Transfusion Medicine

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ObjectivesCold storage is being implemented as an alternative to conventional room‐temperature storage for extending the shelf‐life of platelet components beyond 5–7 days. The aim of this study was to characterise the lipid profile of platelets stored under standard room‐temperature or cold (refrigerated) conditions.MethodsMatched apheresis derived platelet components in 60% PAS‐E/40% plasma (n = 8) were stored at room‐temperature (20–24°C with agitation) or in the cold (2–6°C without agitation). Platelets were sampled on day 1, 5 and 14. The lipidome was assessed by ultra‐pressure liquid chromatography ion mobility quadrupole time of flight mass spectrometry (UPLC IMS QToF). Changes in bioactive lipid mediators were measured by ELISA.ResultsThe total phospholipid and sphingolipid content of the platelets and supernatant were 44 544 ± 2915 μg/mL and 38 990 ± 10 880 μg/mL, respectively, and was similar over 14 days, regardless of storage temperature. The proportion of the procoagulant lipids, phosphatidylserine (PS) and phosphatidylethanolamine (PE), increased by 2.7% and 12.2%, respectively, during extended cold storage. Cold storage for 14 days increased sphingomyelin (SM) by 4.1% and decreased ceramide by 1.6% compared to day 1. Further, lysophosphatidylcholine (LPC) species remained unchanged during cold storage for 14 days. The concentration of 12‐ and 15‐hydroxyeicosatetraenoic acid (HETE) were lower in the supernatant of cold‐stored platelets than room‐temperature controls stored for 14 days.ConclusionThe lipid profile of platelets was relatively unchanged during storage for 5 days, regardless of temperature. However, during extended cold storage (14 days) the proportion of the procoagulant lipids, PS and PE, increased, while LPC and bioactive lipids were stable.

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Cold-stored platelet hemostatic capacity is maintained for three weeks of storage and associated with taurine metabolism

Cited by 4 publications

References 51 publications

Bacterial Contamination of Platelet Products

Bacterial Contamination of Platelet Products

Early Cold Stored Platelet Transfusion Following Severe Injury

Lipidomic changes occurring in platelets during extended cold storage

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