Resistance to chilling injury (Cl) of Florida grapefruit has been found to be seasonal, being most resistant in midseason, and a hypothesis that growth regulators (GRs) are involved in resistance to Cl was proposed. The relationship between resistance to Cl and levels of endogenous GRs in the flavedo of 'Marsh' grapefruit was studied to test the hypothesis. A useful, reliable and precise high-performance liquid chromatographic method, coupled with isotope dilution, was developed for routine quantitative analyses of GRs. Resistance to Cl and levels of endogenous indoleacetic (IAA) and abscisic (ABA) acids were determined for fruit samples harvested biweekly or after 3-day conditioning (delayed storage) treatments under several conditions. vi