Cold Storage and Cryopreservation Methods For Spermatozoa of the sea urchinLytechinus pictus
Victor D. Vacquier,
Amro Hamdoun
Abstract:Sea urchins have contributed greatly to knowledge of fertilization, embryogenesis and cell biology. However, until now, they have not been a genetic model organism because of the long generation times of commonly used species, and lack of tools for husbandry and genetic manipulation. We recently establishedLytechinus pictus, as a multigenerational sea urchin model, because of its relatively short generation time of 4-6 months and ease of laboratory culture. To take full advantage of this new multigenerational … Show more
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