Cold stimulation causes oxidative stress, inflammatory response and apoptosis in broiler heart via regulating Nrf2/HO-1 and NF-κB pathway

Wei, Haidong; Li, Tingting; Zhang, Yong; Liu, Xiaotao; Gong, Rixin; Bao, Jun; Li, Jianhong

doi:10.1016/j.jtherbio.2023.103658

Cited by 17 publications

(14 citation statements)

References 36 publications

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“…Under normal circumstances, Nrf2 is bound to a cytoplasmatic protein called KEAP1, which targets Nrf2 for ubiquitination and subsequent degradation, maintaining low levels of Nrf2 in the cell. On the other hand, under stress conditions, like the LPS challenge in the present study, KEAP1 undergoes a conformational change and thus, does not target Nrf2 for degradation [ 13 , 52 , 53 ]. Nrf2 activation occurs after its translocation into the nucleus, where it binds to the antioxidant response element (ARE) to promote heme oxygenase 1 (HO-1) and other antioxidant gene expression [ 13 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Hydroxytyrosol Supplementation on Growth Performance, Gut Morphometry, and Oxidative and Inflammatory Status in LPS-Challenged Broilers

Dias,

Oliveira,

Calderano

et al. 2024

Animals

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This study assessed the effects of hydroxytyrosol (HT) on 8- to 20-day-old broilers challenged with lipopolysaccharide (LPS); 180 Cobb500™ male chicks were randomly assigned to 3 treatment groups, each comprising 10 replicates with 6 birds per replicate. Treatments included a control diet (CON), CON with LPS administration, and CON + LPS supplemented with 10 mg of HT/kg of feed. LPS was administered intraperitoneally on days 14, 16, 18, and 20. Body weight (BW), body weight gain (BWG), and the feed conversion ratio (FCR) were measured. On day 20, ten birds per treatment were slaughtered for analysis. Bursa, spleen, and liver were collected, and their respective relative weight was determined. The jejunum was destined for morphological analyses of villus height (VH), crypt depth (CD), and their ratio (VH:CD), and for mRNA expression of nuclear factor kappa B (NF-κB), catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and interleukins 10 (IL-10), 1 beta (IL-1β), and 8 (IL-8). HT improved BW, BWG, and FCR, and reduced crypt depth (CD) while increasing the VH:CD ratio in the jejunum. Moreover, HT downregulated mRNA expression of CAT, GPx, IL-10, and IL-1β. In conclusion, HT enhances broiler growth performance, mitigates jejunal mucosa damage from LPS, and modulates antioxidant and immune responses.

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Section: Discussionmentioning

confidence: 99%

Dietary Hydroxytyrosol Supplementation on Growth Performance, Gut Morphometry, and Oxidative and Inflammatory Status in LPS-Challenged Broilers

Dias,

Oliveira,

Calderano

et al. 2024

Animals

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“…Afterward, the tissues were embedded in paraffin and sectioned into sections with a 5-μm thickness using a microtome. The sections were stained with H&E solution, and observed and evaluated under an optical microscope (Eclipse E100, Nikon, Japan) ( Wei et al, 2023a , b ). In stained sections, the nucleus is stained in blue and the cytoplasm appears red under the microscope.…”

Section: Methodsmentioning

confidence: 99%

“…The signal was detected using an enhanced chemiluminescence kit (Beyotime, China), and protein bands were visualized using a grayscale scanner (Gene-Gnome XRQ, Cambridge, UK). Finally, the intensities of protein bands were analyzed using Image-Pro Plus 6.0 software (Media Cybernetic, MD), and the protein expression level was expressed as the ratio of the optical density of each target protein to that of β-actin ( Wei et al, 2023a ).…”

Section: Methodsmentioning

confidence: 99%

“…Numerous studies have reported a significant reduction in production performance and body weight gain of chickens under cold stress ( Zhang et al, 2016 ; Olfati et al, 2018 ). Cold stress causes oxidative stress, metabolic abnormalities, immunologic dysfunction, and tissue injury in chickens, which impairs health ( Mujahid and Furuse, 2009 ; Su et al, 2020 ; Wei et al, 2023a ). Additionally, cold stress has been associated with the development and exacerbation of respiratory diseases, such as lung damage, pneumonia, and respiratory infections in rats and broilers ( Luo et al, 2019 ; Yu et al, 2023 ), thereby affecting respiratory function.…”

Section: Introductionmentioning

confidence: 99%

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Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers

Wei,

Zhang,

et al. 2024

Poultry Science

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“…At present, the high-density intensive feeding model makes broilers who are highly sensitive to various stimuli prone to oxidative stress and immune damage, as well as increasing the incidence and transmission risk of various infectious diseases [ 1 , 2 , 3 ]. During oxidative stress, free radicals increase in the body, and the body is in a peroxidation state, which will cause oxidative damage to cells, tissues, and organs [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Terminalia chebula Extract on Growth Performance, Immune Function, Antioxidant Capacity, and Intestinal Health of Broilers

Cheng,

Liu,

Wang

et al. 2024

Animals

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Terminalia chebula extract (TCE) has many physiological functions and is potentially helpful in maintaining poultry health, but its specific effect on the growth of broilers is not yet known. This research investigated the effects of dietary Terminalia chebula extract (TCE) supplementation on growth performance, immune function, antioxidant capacity, and intestinal health in yellow-feathered broilers. A total of 288 one-day-old yellow-feathered broilers were divided into four treatment groups (72 broilers/group), each with six replicates of 12 broilers. The broilers were given a basal diet of corn–soybean meal supplemented with 0 (control), 200, 400, and 600 mg/kg TCE for 56 d. The results demonstrated that, compared with the basal diet, the addition of TCE significantly increased (linear and quadratic, p < 0.05) the final body weight and overall weight gain and performance and decreased (linear and quadratic, p < 0.05) the feed-to-gain ratio in the overall period. Dietary TCE increased (linear, p < 0.05) the levels of IgM, IL-4, and IL-10 and decreased (linear and quadratic, p < 0.05) the level of IL-6 in the serum. Dietary TCE increased (linear and quadratic, p < 0.05) the levels of IL-2 and IL-4, decreased (linear and quadratic, p < 0.05) the level of IL-1β, and decreased (linear, p < 0.05) the level of IL-6 in the liver. Dietary TCE increased (linear and quadratic, p < 0.05) the level of IgM and IL-10, increased (linear, p < 0.05) the level of IgG, and decreased (linear and quadratic, p < 0.05) the levels of IL-1β and IL-6 in the spleen. Supplementation with TCE linearly and quadratically increased (p < 0.05) the catalase, superoxide dismutase, glutathione peroxidase, and total antioxidant capacity activities while decreasing (p < 0.05) the malonic dialdehyde concentrations in the serum, liver, and spleen. TCE-containing diets for broilers resulted in a higher (linear and quadratic, p < 0.05) villus height, a higher (linear and quadratic, p < 0.05) ratio of villus height to crypt depth, and a lower (linear and quadratic, p < 0.05) crypt depth compared with the basal diet. TCE significantly increased (linear, p < 0.05) the acetic and butyric acid concentrations and decreased (quadratic, p < 0.05) the isovaleric acid concentration. Bacteroidaceae and Bacteroides, which regulate the richness and diversity of microorganisms, were more abundant and contained when TCE was added to the diet. In conclusion, these findings demonstrate that supplementing broilers with TCE could boost their immune function, antioxidant capacity, and gut health, improving their growth performance; they could also provide a reference for future research on TCE.

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Cold stimulation causes oxidative stress, inflammatory response and apoptosis in broiler heart via regulating Nrf2/HO-1 and NF-κB pathway

Cited by 17 publications

References 36 publications

Dietary Hydroxytyrosol Supplementation on Growth Performance, Gut Morphometry, and Oxidative and Inflammatory Status in LPS-Challenged Broilers

Dietary Hydroxytyrosol Supplementation on Growth Performance, Gut Morphometry, and Oxidative and Inflammatory Status in LPS-Challenged Broilers

Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers

Effects of Dietary Terminalia chebula Extract on Growth Performance, Immune Function, Antioxidant Capacity, and Intestinal Health of Broilers

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