Cold Preservation of the Liver With Oxygenation by a Two-Layer Method

Odaira, Masanori; Aoki, Takeshi; Miyamoto, Yoichi; Yasuhara, Rika; Jin, Zhenghao; Yu, Junhong; Nishino, Nobukazu; Yamada, Kunio; Kusano, Tomokazu; Hayashi, Ken; Yasuda, Daisuke; Koizumi, Toru; Mitamura, Keiji; Enami, Yuta; Niiya, Takashi; Murai, Noriyuki; Kato, Hirohisa; Shimizu, Yoshinori; Kamijyo, Ryutaro; Kusano, Mitsuo

doi:10.1016/j.jss.2008.03.034

Cited by 3 publications

(2 citation statements)

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“…Our preliminary studies have already shown that fresh liver tissue that has undergone cold ischemic damage can be salvaged by TLM after cold storage [40]. Furthermore, rat liver preservation using TLM compared with the UW solution also generated superior outcomes in the present study.…”

Section: Discussionmentioning

confidence: 49%

“…The optimal conditions for liver preservation rely on an adequate supply of oxygen. PFD is a unique, inert, colorless fluid that has the appearance and consistency of water; it can maintain high partial pressures of oxygen for extended periods, and oxygen can be offloaded to areas of lower partial pressure [40]. In this study, TLM was developed for pancreas preservation using UW and PFC solutions.…”

Section: Discussionmentioning

confidence: 99%

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Oxygenated Static Preservation of Donation after Cardiac Death Liver Grafts Improves Hepatocyte Viability and Function

Murakami²,

Aoki³

et al. 2015

Eur Surg Res

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Background: Cell therapy, such as hepatocyte transplantation (HTx), is promising for the treatment of metabolic liver diseases or as a bridge to orthotopic liver transplantation in patients with fulminant liver failure. However, one of the limitations of this therapy is the shortage of donors. The present study aims to investigate whether the two-layer method (TLM) of cold preservation with oxygenation improves the viability and activity of hepatocytes from rat donation after cardiac death (DCD) donors compared with results obtained with the University of Wisconsin (UW) solution. Moreover, we evaluated the hepatocyte function after culture or transplantation into the spleen. Materials and Methods: We used male Sprague-Dawley rats for this study. The DCD model was induced by phrenotomy after injecting heparin. We assigned rats based on warm ischemia times of 15 and 30 min to groups S and L, respectively. Each group (n = 5) was then subdivided as follows: (1) group S: not preserved (S/N), preserved by TLM for 3 h (S/TLM3) and 12 h (S/TLM12), and in the UW solution for 3 h (S/UW3) and 12 h (S/UW12), and (2) group L: not preserved (L/N), preserved by TLM for 3 h (L/TLM3) and 12 h (L/TLM12), and in the UW solution for 3 h (L/UW3) and 12 h (L/UW12). The cell viability and function of isolated DCD hepatocytes were analyzed for culture or HTx into the spleen. Results: The viability and ATP levels of DCD hepatocytes significantly improved after TLM compared with the values after preservation in cold UW solution in group S/N (p < 0.059). The levels of albumin production and urea synthesis by hepatocytes after culture were significantly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12 (p < 0.05), respectively. Further, serum albumin levels after HTx were also markedly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12. The morphological features revealed that cultured and transplanted hepatocytes remained clearly viable and maintained an expression for specific hepatic function, such as the production of albumin and glycogen. Conclusion: This novel method of oxygenated cold preservation of DCD livers can expand the hepatocyte donor pool for HTx and establish a wider application of this developing technique.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 99%