Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana

Chowański, Szymon; Lubawy, Jan; Spochacz, Marta; Ewelina, Paluch; Grzegorz, Smykalla; Rosiński, Grzegorz; Słocińska, Małgorzata

doi:10.1016/j.cbpa.2015.01.007

Cited by 36 publications

(39 citation statements)

References 63 publications

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“…Climatic conditions also change abundance of energy reserves (Chowanski et al 2015). F. auricularia is univoltine, and in south of France, earwigs reach the adult stage in early summer.…”

Section: Discussionmentioning

confidence: 99%

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An exploratory study of energy reserves and biometry as potential tools for assessing the effects of pest management strategies on the earwig, Forficula auricularia L

Suchail

Navenant

Capowiez

et al. 2018

Environ Sci Pollut Res

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Apple orchards are heavily treated crops and some sprayed insecticides are recognized to have toxic effects on non-target arthropods. Earwigs are important natural enemies in pip-fruit orchards and contribute to the biological control of aphids. In addition, due to their ease of capture and identification, they are an interesting potential bioindicator of the possible detrimental effects of different orchard management strategies. In this study, we measured the energy reserves and some morphological traits of Forficula auricularia L. sampled in apple orchards under management strategies (organic versus integrated pest management (IPM)). We observed a significant decrease in mass (22 to 27%), inter-eye width (3%), and prothorax width (2 to 5%) in earwigs from IPM compared to organic orchards. Energy body reserves also confirmed these results with a significant decrease of 48% in glycogen and 25 to 42% in lipid content in earwigs from IPM compared to organic orchards. However, the protein content was approximately 70% higher in earwigs from IPM than in organic orchards. Earwigs sampled in IPM orchards may adapt to minimize the adverse toxic effects of pesticide treatments using a large number of strategies, which are reflected in changes to their energy reserves. These strategies could, in turn, influence the population dynamics of natural enemies and impair their role in the biological control of pests in apple orchards.

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“…Climatic conditions also change abundance of energy reserves (Chowanski et al 2015). F. auricularia is univoltine, and in south of France, earwigs reach the adult stage in early summer.…”

Section: Discussionmentioning

confidence: 99%

“…Glycogen can be rapidly hydrolyzed to glucose in the haemolymph of insects to be used as a glycolytic fuel (Steele 1982). The glycogen content may vary depending on physiological state (Anand and Lorenz 2008) or environmental conditions (Chowanski et al 2015).…”

Section: Introductionmentioning

confidence: 99%

An exploratory study of energy reserves and biometry as potential tools for assessing the effects of pest management strategies on the earwig, Forficula auricularia L

Suchail

Navenant

Capowiez

et al. 2018

Environ Sci Pollut Res

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“…The second strategy is much more widespread among arthropod phyla (Block, 1990; Lee and Costanzo, 1998), and freeze-avoiding insects utilize mechanisms which raise their ability to stay unfrozen by supercooling (Sformo et al, 2010). Cold and freezing stress affects a large number of physiological processes (Teets and Denlinger, 2013), causing mechanical damage to cells or their DNA (Lubawy et al, 2019), changes in main metabolic pathways (Chowanski et al, 2015(Chowanski et al, , 2017b or cellular dehydration, which results in increased acidity, toxic metabolic intermediate concentrations and osmotic stress (Storey and Storey, 2012;Pegg, 2015;Des Marteaux and Sinclair, 2016;Andersen et al, 2018). In both of these strategies, cryoprotectants are necessary for survival.…”

Section: Introductionmentioning

confidence: 99%

Role of the Insect Neuroendocrine System in the Response to Cold Stress

et al. 2020

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Insects are the largest group of animals. They are capable of surviving in virtually all environments from arid deserts to the freezing permafrost of polar regions. This success is due to their great capacity to tolerate a range of environmental stresses, such as low temperature. Cold/freezing stress affects many physiological processes in insects, causing changes in main metabolic pathways, cellular dehydration, loss of neuromuscular function, and imbalance in water and ion homeostasis. The neuroendocrine system and its related signaling mediators, such as neuropeptides and biogenic amines, play central roles in the regulation of the various physiological and behavioral processes of insects and hence can also potentially impact thermal tolerance. In response to cold stress, various chemical signals are released either via direct intercellular contact or systemically. These are signals which regulate osmoregulationcapability peptides (CAPA), inotocin (ITC)-like peptides, ion transport peptide (ITP), diuretic hormones and calcitonin (CAL), substances related to the general response to various stress factors-tachykinin-related peptides (TRPs) or peptides responsible for the mobilization of body reserves. All these processes are potentially important in cold tolerance mechanisms. This review summarizes the current knowledge on the involvement of the neuroendocrine system in the cold stress response and the possible contributions of various signaling molecules in this process.

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“…Thus, they play a role in the regulation of the osmotic potential of extra- and intracellular fluid, which influences the efficiency of ice crystal growth. Insects experiencing low temperatures have also exhibited changes in the level of AQP expression [ 30 ]. The role of AQPs in cold resistance was confirmed by Izumi et al [ 31 ] and Zelenina et al [ 32 ], who showed that when AQPs are blocked by mercury or copper, the resistance of insect cells to cold stress decreases.…”

Section: Introductionmentioning

confidence: 99%

The physiological role of fat body and muscle tissues in response to cold stress in the tropical cockroach Gromphadorhina coquereliana

et al. 2017

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Protective mechanisms against cold stress are well studied in terrestrial and polar insects; however, little is known about these mechanisms in tropical insects. In our study, we tested if a tropical cockroach Gromphadorhina coquereliana, possesses any protective mechanisms against cold stress. Based on the results of earlier studies, we examined how short-term (3 h) cold (4°C) influences biochemical parameters, mitochondrial respiration activity, and the level of HSPs and aquaporins expression in the fat body and leg muscles of G. coquereliana. Following cold exposure, we found that the level of carbohydrates, lipids and proteins did not change significantly. Nevertheless, we observed significant changes in mitochondrial respiration activity. The oxygen consumption of resting (state 4) and phosphorylating (state 3) mitochondria was altered following cold exposure. The increase in respiratory rate in state 4 respiration was observed in both tissues. In state 3, oxygen consumption by mitochondria in fat body was significantly lower compared to control insects, whereas there were no changes observed for mitochondria in muscle tissue. Moreover, there were cold-induced changes in UCP protein activity, but the changes in activity differed in fat body and in muscles. Additionally, we detected changes in the level of HSP70 and aquaporins expression. Insects treated with cold had significantly higher levels of HSP70 in fat body and muscles. On the other hand, there were lower levels of aquaporins in both tissues following exposure to cold. These results suggest that fat body play an important role in protecting tropical insects from cold stress.

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Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana

Cited by 36 publications

References 63 publications

An exploratory study of energy reserves and biometry as potential tools for assessing the effects of pest management strategies on the earwig, Forficula auricularia L

An exploratory study of energy reserves and biometry as potential tools for assessing the effects of pest management strategies on the earwig, Forficula auricularia L

Role of the Insect Neuroendocrine System in the Response to Cold Stress

The physiological role of fat body and muscle tissues in response to cold stress in the tropical cockroach Gromphadorhina coquereliana

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