Cold hardiness estimation of Pinus densiflora var. zhangwuensis based on changes in ionic leakage, chlorophyll fluorescence and other physiological activities under cold stress

Meng, Ping; Bai, Xuefeng; Li, Hongdan; Song, Xiaodong; Zhang, Xueli

doi:10.1007/s11676-015-0111-3

Cited by 14 publications

(6 citation statements)

References 25 publications

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“…Membrane permeability and relative conductivity (REC) were measured as described by Daneshmand et al (2010) and Meng et al (2015) , respectively. Reactive oxygen species were determined by the method of Wang and Jiao (2000) .…”

Section: Methodsmentioning

confidence: 99%

Transcriptome sequencing and gene expression profiling of <i>Pinus sibirica</i> under different cold stresses

et al. 2021

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Cold stress is a major abiotic factor that affects plant growth and geographical distribution. Pinus sibirica is extremely frigostable tree species. To understand the molecular mechanisms of cold tolerance by P. sibirica, physiological responses were analyzed and transcriptome profiling was conducted to the plants treated by cold stress. The physiological data showed that membrane permeability relative conductivity (REC), reactive oxygen species (ROS), malonaldehyde (MDA) content, peroxidase (POD) and catalase (CAT) activity, soluble sugar, soluble protein and proline contents were increased significantly (p < 0.05) in response to cold stress. Transcriptome analysis identified a total of 871, 1397 and 872 differentially expressed genes (DEGs) after cold treatment for 6 h, 24 h and 48 h at -20°C, respectively. The signaling pathway mediated by Ca 2+ as a signaling molecule and abscisic acid pathways were the main cold signal transduction pathways in P. sibirica. The APETALA2/Ethylene-Responsive Factor (AP2/ERF) and MYB transcription factor families also play an important role in the transcriptional regulation of P. sibirica. In addition, many genes related to photosynthesis were differentially expressed under cold stress. We also validated the reliability of transcriptome data with quantitative real-time PCR. This study lays the foundation for understanding the molecular mechanisms related to cold responses in P. sibirica.

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Section: Methodsmentioning

confidence: 99%

Transcriptome sequencing and gene expression profiling of <i>Pinus sibirica</i> under different cold stresses

et al. 2021

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“…Studies have focused on nutrient cycling, root system architecture, antioxidant activity, and water-deficiency responses in inoculated plants [17,18]. Nevertheless, studies have reported the sensitivity and acclimation of photosynthesis in masson pine, with particular focus on chlorophyll (Chl) a fluorescence, which is a quick method to estimate the photosynthetic response in plants under various environmental conditions [19], including water deficiency [20], cold hardiness [21], and toxic metals [22].…”

Section: Introductionmentioning

confidence: 99%

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Wang

Zhao

et al. 2021

Forests

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Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings' growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased An/gs and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.

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“…Our model correctly simulated the pathway of conversion from starch to sugar in fall and the opposite process in spring (Tixier et al ., 2019; Deslauriers et al ., 2021). During autumn, when decreasing photoperiod and temperature induce both endo‐ and ecodormancy (Singh et al ., 2017), the exposure to chilling promotes sugar accumulation in all living cells, the sugars being mainly sucrose and some other oligosaccharides (Meng et al ., 2015; Taïbi et al ., 2018; Deslauriers et al ., 2021). Our simulated starch and sugar patterns during winter agree with the literature (Schaberg et al ., 2000), where the accumulated sugar can increase to > 25 times the concentrations observed during summer (Strimbeck et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

PhenoCaB: a new phenological model based on carbon balance in boreal conifers

Cartenì

Balducci

Dupont³

et al. 2023

New Phytologist

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Summary Traditional phenological models use chilling and thermal forcing (temperature sum or degree‐days) to predict budbreak. Because of the heightening impact of climate and other related biotic or abiotic stressors, a model with greater biological support is needed to better predict budbreak. Here, we present an original mechanistic model based on the physiological processes taking place before and during budbreak of conifers. As a general principle, we assume that phenology is driven by the carbon status of the plant, which is closely related to environmental variables and the annual cycle of dormancy–activity. The carbon balance of a branch was modelled from autumn to winter with cold acclimation and dormancy and from winter to spring when deacclimation and growth resumption occur. After being calibrated in a field experiment, the model was validated across a large area (> 34 000 km2), covering multiple conifers stands in Québec (Canada) and across heated plots for the SPRUCE experiment in Minnesota (USA). The model accurately predicted the observed dates of budbreak in both Québec (±3.98 d) and Minnesota (±7.98 d). The site‐independent calibration provides interesting insights on the physiological mechanisms underlying the dynamics of dormancy break and the resumption of vegetative growth in spring.

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Cold hardiness estimation of Pinus densiflora var. zhangwuensis based on changes in ionic leakage, chlorophyll fluorescence and other physiological activities under cold stress

Cited by 14 publications

References 25 publications

Transcriptome sequencing and gene expression profiling of <i>Pinus sibirica</i> under different cold stresses

Transcriptome sequencing and gene expression profiling of <i>Pinus sibirica</i> under different cold stresses

Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

PhenoCaB: a new phenological model based on carbon balance in boreal conifers

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