Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete – Isaria farinosa

Linke, Diana; Riemer, Stephanie J.L.; Schimanski, Silke; Nieter, Annabel; Krings, Ulrich; Berger, Ralf G.

doi:10.1016/j.funbio.2017.05.006

Cited by 10 publications

(19 citation statements)

References 49 publications

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“…farinosa 2 , the study of I . farinosa intensified, including not only its virulence characteristics 3 , 4 , fungicide 5 , 6 , biochemical characterisation 7 , biotransformation activity 8 and molecular biology 9 , 10 but also its secondary metabolites and pharmacological activity 11 .…”

Section: Introductionmentioning

confidence: 99%

Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome

Liu

Xiang

Guo

et al. 2018

Sci Rep

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Isaria farinosa is a pathogen of alpine Thitarodes larvae that are hosts for the Chinese medicinal fungus, Ophiocordyceps sinensis. A matrix analysis indicated that the optimal culture conditions for the mycelial growth of I. farinosa are a 50-mL liquid broth in a 250-mL flask at more than 100-rpm rotation and 15–25 °C. Illumination does not affect the mycelial growth. The optimal nutrition requirements are D-(+)-galactose and D-(−)-fructose as carbon resources and D-cysteine as well as yeast powder, peptone, and beef extract as nitrogen resources at a carbon-to-nitrogen ratio of 1:1 to 1:7. The mineral component and vitamins also significantly increase the mycelial growth of I. farinosa. Based on the optimal culture conditions and nutrition requirements for the mycelial growth of I. farinosa, the effects of altitude on mycelial growth and its metabolome were evaluated using quadrupole-time-of-flight/mass spectrometry, principal component analysis and partial least squares discriminant analysis. The altitude did not affect the mycelial production but significantly regulated its metabolome. The study presents a new approach to better select a method for producing more useful metabolites and highlights the necessity of establishing standards for culturing methods related to altitude to preserve fungal quality; additionally, the results indicate that the use of a fermenter may meet the demands of large-scale mycelial production.

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Section: Introductionmentioning

confidence: 99%

Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome

Liu

Xiang

Guo

et al. 2018

Sci Rep

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“…The closest relatives producing analogous enzymes are ascomycetes, the second main class of higher fungi. Examples are the well-studied FDC1 from Saccharomyces cerevisiae, the AlPAD from Aspergillus luchuensis and the recently described IfaPAD from Cordyceps farinosa [17,22,26,27]. [25] using the template of a phenolic acid decarboxylase from Bacillus pumilus (3nad.1) described by Matte et al [24].…”

Section: Discussionmentioning

confidence: 99%

“…All measurements were obtained from triplets of independent samples. The purity was confirmed by GC-MS as described previously [17].…”

Section: Decarboxylase Activity Assay and Hplcmentioning

confidence: 99%

“…The protein sequence of the ScoFAD was aligned to the first phenolic acid decarboxylase from a filamentous ascomycete recently described by Linke et al [17] and an enterobacterial FAD described by Gu et al [8] (Fig 4). The ScoFAD (Sco) exhibited a sequence identity of 19.4% to the FAD from Enterobacter sp.…”

Section: Protein Modellingmentioning

confidence: 99%

“…https://doi.org/10.1371/journal.pone.0244290.g003 MT459803), an ascomycetous p-coumaric acid decarboxylase from Cordyceps farinosa described by Linke et al [17] (Cfa, CUI18215), and two bacterial ferulic acid decarboxylases from Enterobacter sp. studied by Gu et al [8] (Esp, ACJ26748) and from Bacillus pumilus by Matte et al [24] (Bpu, Q45361) were aligned by Clustal W. Conserved residues are marked in black, conservative mutations in dark-grey and semiconservative mutations in light-grey.…”

Section: Enzyme Immobilisationmentioning

confidence: 99%

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Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

2020

Self Cite

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Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol (4-VG), the impact compound of smoke flavour. The first basidiomycetous ferulic acid decarboxylase (FAD) was isolated from Schizophyllum commune (ScoFAD) and heterologously produced by Komagataella phaffii. It showed a molecular mass of 21 kDa, catalytic optima at pH 5.5 and 35°C, and a sequence identity of 63.6% to its next relative, a FAD from the ascomycete Cordyceps farinosa. The ScoFAD exhibited a high affinity to its only known substrate ferulic acid (FA) of 0.16 mmol L-1 and a turnover number of 750 s-1. The resulting catalytic efficiency kcat KM-1 of 4,779 L s-1 mmol-1 exceeded the next best known enzyme by more than a factor of 50. Immobilised on AminoLink Plus Agarose, ScoFAD maintained its activity for several days. The combination with FAEs and agro-industrial side-streams paves the way for a new generation of sustainable, clean, and safe smoke flavours.

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Metabolomics and Therapeutic Potential of Ophiocordyceps sinensis

Bhardwaj¹,

Rajaura²,

Chauhan³

et al. 2022

Phytochemical Genomics

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Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete – Isaria farinosa

Cited by 10 publications

References 49 publications

Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome

Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome

Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Metabolomics and Therapeutic Potential of Ophiocordyceps sinensis

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