1996
DOI: 10.1006/jmbi.1996.0359
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Cold Denaturation of Barstar:1H,15N and13C NMR Assignment and Characterisation of Residual Structure

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Cited by 104 publications
(108 citation statements)
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“…13 However, spectra recorded at low and high temperatures are very different: the spectrum at − 1°C is well populated and informative, even though most of the nuclear Overhauser enhancement effects are intra-residue or sequential as previously reported. 8 The 50°C nuclear Overhauser enhancement spectroscopy-HSQC spectrum contains instead very few off-diagonal peaks (Fig.…”
Section: Resultsmentioning
confidence: 69%
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“…13 However, spectra recorded at low and high temperatures are very different: the spectrum at − 1°C is well populated and informative, even though most of the nuclear Overhauser enhancement effects are intra-residue or sequential as previously reported. 8 The 50°C nuclear Overhauser enhancement spectroscopy-HSQC spectrum contains instead very few off-diagonal peaks (Fig.…”
Section: Resultsmentioning
confidence: 69%
“…Ethylenediaminetetraacetic acid and salts were removed by dialysis prior to concentration of the protein. 15 Nlabeled and 15 N, 13 C-double-labeled samples were produced by growing the bacteria in minimal medium using labeled ammonium sulfate and glucose as the sole source of nitrogen and carbon.…”
Section: Experimental Procedures Sample Preparationmentioning
confidence: 99%
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“…Recent experimental data suggest that unfolded states of proteins are ensembles of rapidly interconverting, structurally and dynamically diverse conformers that may be fairly compact and exhibit considerable amounts of nonrandom structure. Residual secondary and tertiary structure can be retained in unfolded states, involving specific local and longrange interactions, some of which are native-like and some of which are not observed in the native (folded) structure (1)(2)(3)(4)(5).…”
mentioning
confidence: 99%
“…21 Other experiments, studying urea-unfolded Barstar, also find residual structure within the unfolded conformations. For example, five residues in Helix 1 show propensity towards populating helical regions in (φ,ψ) space.…”
Section: This Studymentioning
confidence: 97%