Neutral atoms stored in optical traps are strong candidates for a physical realization of a quantum logic device 1,2 . Far off-resonance optical traps provide conservative potentials and excellent isolation from the environment, and they may be arranged to produce arbitrary arrays of traps, where each trap is occupied by a single atom that can be individually addressed [3][4][5][6] . At present, significant effort is being expended on developing two-qubit gates based on coupling individual Rydberg atoms in adjacent optical microtraps [7][8][9] . A major challenge associated with this approach is the reliable generation of single-atom occupancy in each trap, as the loading efficiency in the past experiments has been limited to 50% (refs 4,7,8,10-12). Here we report a loading efficiency of 82.7% in an optical microtrap. We achieve this by manipulating the collisions between pairs of trapped atoms through tailored optical fields and directly observing the resulting single atoms in the trap.Deterministic control of single neutral atoms is a long-standing goal in atomic physics. Not only would it represent a milestone in scientists' ability to control the microscopic world, but also because it would enable a neutral-atom-based quantum logic device 7-9 . Two approaches have successfully led to direct observation of subPoissonian number distributions of atoms in optical microtraps, without consecutive atom sorting 13 . In the first, the Mott insulator transition of a Bose-Einstein condensate provides an efficient route for high occupancy of individual atoms in optical lattices where atoms can tunnel between adjacent lattice sites [14][15][16][17] . The second approach, which may be applied in arbitrary geometries 18,19 , is to employ light-assisted collisions 4,[10][11][12] . This method makes use of the change in the atom-atom interaction that arises when light drives one of the atoms undergoing a collision to the electronic excited state. In the case of light with a frequency below resonance (red detuned), the atom pair is excited to an attractive potential leading to the atoms forming a molecule and/or gaining a large amount of kinetic energy. In each case, both atoms are lost, leading to a maximal 50% chance of ending with one atom in the trap, depending on whether the initial atom number is even or odd 10,12 . However, a process where only one atom is lost as a result of a two-body collision would lead to deterministic preparation of a single atom in a given site. In the past, it has been shown that various forms of collisional trap loss can be suppressed by the application of optical control fields, and in particular, the use of blue-detuned light to effect so-called optical shielding 20,21 . In this Letter, we study light-assisted collisions at the single-event level. We prepare individual pairs of atoms in an optical microtrap and expose them to near-resonant light. We directly observe that light-assisted collisions between these atoms can lead to only one atom being lost. By choice of the frequency and intensi...