Cold atmospheric pressure plasma jet interactions with plasmid DNA

Abstract

The effect of a cold (<40 °C) radio frequency-driven atmospheric pressure plasma jet on plasmid DNA has been investigated. Gel electrophoresis was used to analyze the DNA forms post-treatment. The experimental data are fitted to a rate equation model that allows for quantitative determination of the rates of single and double strand break formation. The formation of double strand breaks correlates well with the atomic oxygen density. Taken with other measurements, this indicates that neutral components in the jet are effective in inducing double strand breaks.

Experimental Study of Atmospheric Pressure Argon Plasma Jet-Induced Strand Breakage in Large DNA Molecules

“…13,23 Similar results in PBS and water were reported by Leduc et al, 32 although the effect in TE was not observed. PS, which consists of dilute sodium chloride solution (0.85%), showed DNA fragreported to form in distilled water when it was exposed to all types of plasma.…”

“…6-11 12 biomolecules before its potential application on complex structures such as living tissue can of the DNA repair mechanism or DNA damage in the microorganism; the plasma jet has been shown to induce damage to cellular and plasmid DNA. 13 Study of the plasma jet in [14][15][16] The medium used for suspension of biomolecules and microorganisms during 1,17 Damage has been shown to be due to the interaction of biomolecules with reactive oxygen species of the cellular medium, because plasma modulates vital properties of media, and the toxic nature of plasma can also be altered by the surrounding media.…”

Jet‐to‐jet interactions in atmospheric‐pressure plasma jet arrays for surface processing

“…can diffuse relatively uniformly in the hydrodynamic flow in Schlieren photographs. [26,36] The reactive oxygen radicals are essential for bio-medical surface treatments [37,38] and OH and O can form oxygen-containing hydrophilic polar group on the surface and increase the hydrophilicity of polymer surface. [39,40] The O 3 can cause damage to mucous and respiratory tissues in animals and also tissues in plants and NO is necessary for macrophages acting against microorganisms and tumors.…”

Generation and loss of reactive oxygen species in low-temperature atmospheric-pressure RF He + O2 + H2O plasmas

“…A large number of plasma sources and biological targets have been investigated to date and there is growing amount of literature that suggests that for low-temperature atmospheric-pressure plasmas the interaction with biological targets is mainly chemistry driven [3,4,5]. UV radiation and physical etching observed in low-pressure plasma treatments [6,7] do not seem to play a dominant role at atmospheric pressure.…”