Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages

Duchesne, Constance; Frescaline, Nadira; Blaise, Océane; Lataillade, Jean‐Jacques; Banzet, Sébastien; Dussurget, Olivier; Rousseau, Antoine

doi:10.1128/msphere.00217-21

Cited by 13 publications

(8 citation statements)

References 50 publications

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“…In turn, Yoshikawa et al demonstrated autophagy arising in the cells cultured with CAP-activated medium and also argued that autophagy was the death pathway of treated cells [20]. Recently, Duchesne et al [57] showed that CAP treatment promoted macrophages' ability to eliminate internalized bacteria by promoting the association of bacteria to LAMP-1-positive phagosomes, in which bacteria were exposed to low pH and cathepsin D hydrolase.…”

Section: Discussionmentioning

confidence: 99%

Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

Patrakova

Biryukov

Troitskaya

et al. 2023

Cells

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Cold atmospheric plasma (CAP) is an intensively-studied approach for the treatment of malignant neoplasms. Various active oxygen and nitrogen compounds are believed to be the main cytotoxic effectors on biotargets; however, the comprehensive mechanism of CAP interaction with living cells and tissues remains elusive. In this study, we experimentally determined the optimal discharge regime (or semi-selective regime) for the direct CAP jet treatment of cancer cells, under which lung adenocarcinoma A549, A427 and NCI-H23 cells demonstrated substantial suppression of viability, coupled with a weak viability decrease of healthy lung fibroblasts Wi-38 and MRC-5. The death of CAP-exposed cancer and healthy cells under semi-selective conditions was caspase-dependent. We showed that there was an accumulation of lysosomes in the treated cells. The increased activity of lysosomal protease Cathepsin D, the transcriptional upregulation of autophagy-related MAPLC3B gene in cancer cells and the changes in autophagy-related proteins may have indicated the activation of autophagy. The addition of the autophagy inhibitor chloroquine (CQ) after the CAP jet treatment increased the death of A549 cancer cells in a synergistic manner and showed a low effect on the viability of CAP-treated Wi-38 cells. Downregulation of Drp1 mitochondrial protein and upregulation of PINK1 protein in CAP + CQ treated cells indicated that CQ increased the CAP-dependent destabilization of mitochondria. We concluded that CAP weakly activated pro-survival autophagy in irradiated cells, and CQ promoted CAP-dependent cell death due to the destabilization of autophagosomes formation and mitochondria homeostasis. To summarize, the combination of CAP treatment with CQ could be useful for the development of cold plasma-based antitumor approaches for clinical application.

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Section: Discussionmentioning

confidence: 99%

Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

Patrakova

Biryukov

Troitskaya

et al. 2023

Cells

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“…Reactive oxygen and nitrogen species produced by CAP can trigger programmed cell death with hallmarks of apoptosis by short-term treatment, but long-term CAP treatment can physically destruct intracellular components ( 18 ). In addition, CAP may promote the potency of macrophages to kill S. aureus by improving the formation of degradative phagosomes ( 19 ). Besides the anti-inflammatory effects by inhibiting the prostaglandin synthesis, DIC was suggested to affect in vitro bacterial growth by modulating DNA synthesis ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

Reduced Staphylococcus Abundance Characterizes the Lesional Microbiome of Actinic Keratosis Patients after Field-Directed Therapies

Kehrmann,

Koch,

Zumdick

et al. 2023

Microbiol Spectr

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“…Cold atmospheric plasma (CAP), electrical discharged gas, has antimicrobial properties, which a study by Duchesne et al investigated [ 125 ]. It was found that CAP treatment increased intracellular ROS in RAW 264.7 macrophages irrespective of whether the cells were infected with S. aureus .…”

Section: Treatment Options To Manage Intracellular S Aureus...mentioning

confidence: 99%

Intracellular Habitation of Staphylococcus aureus: Molecular Mechanisms and Prospects for Antimicrobial Therapy

Hommes

Surewaard

2022

Biomedicines

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Methicillin-resistant Staphylococcus aureus (MRSA) infections pose a global health threat, especially with the continuous development of antibiotic resistance. As an opportunistic pathogen, MRSA infections have a high mortality rate worldwide. Although classically described as an extracellular pathogen, many studies have shown over the past decades that MRSA also has an intracellular aspect to its infectious cycle, which has been observed in vitro in both non-professional as well as professional phagocytes. In vivo, MRSA has been shown to establish an intracellular niche in liver Kupffer cells upon bloodstream infection. The staphylococci have evolved various evasion strategies to survive the antimicrobial environment of phagolysosomes and use these compartments to hide from immune cells and antibiotics. Ultimately, the host cells get overwhelmed by replicating bacteria, leading to cell lysis and bacterial dissemination. In this review, we describe the different intracellular aspects of MRSA infection and briefly mention S. aureus evasion strategies. We discuss how this intracellular niche of bacteria may assist in antibiotic tolerance development, and lastly, we describe various new antibacterial strategies that target the intracellular bacterial niche.

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Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages

Abstract: Staphylococcus aureus is the most frequent cause of skin and soft tissue infections. Treatment failures are increasingly common due to antibiotic resistance and the emergence of resistant strains.

Cited by 13 publications

References 50 publications

Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

Reduced Staphylococcus Abundance Characterizes the Lesional Microbiome of Actinic Keratosis Patients after Field-Directed Therapies

Intracellular Habitation of Staphylococcus aureus: Molecular Mechanisms and Prospects for Antimicrobial Therapy

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