Cold atmospheric plasma (CAP) surface nanomodified 3D printed polylactic acid (PLA) scaffolds for bone regeneration

Wang, Mian; Favi, Pelagie; Cheng, Xiaoqian; Golshan, Negar H.; Ziemer, Katherine S.; Keidar, Michael; Webster, Thomas J.

doi:10.1016/j.actbio.2016.09.030

Cited by 163 publications

(107 citation statements)

References 26 publications

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“…At 120× magnification it is clear that the extruded PLA at standard (0.2 mm) resolution fused well; however, it can be noted that integrated filaments had a micron and sub-micron scale roughness (which as noted later may be beneficial for cell adhesion and growth when used in scaffolding applications [17,18]). The ridged structure likely results from the movement of the print head during extrusion and cooling, and may be affected by the retention of heat in the sample.…”

Section: Scanning Electron Microscopy (Sem) Resultsmentioning

confidence: 99%

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

et al. 2017

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Featured Application: Detailed chemical and structural changes to polylactid acid (PLA) from source material through 3D printing needs to be quantified in order to use PLA 3D printed structures for specific applications. This work was specifically motivated for specific applications for in situ synthesis of silver nanoparticles on printed PLA surfaces used in antimicrobial applications and the development and characterization of PLA constructs for tissue engineering.Abstract: Polylactic acid (PLA) is an organic polymer commonly used in fused deposition (FDM) printing and biomedical scaffolding that is biocompatible and immunologically inert. However, variations in source material quality and chemistry make it necessary to characterize the filament and determine potential changes in chemistry occurring as a result of the FDM process. We used several spectroscopic techniques, including laser confocal microscopy, Fourier transform infrared (FTIR) spectroscopy and photoacousitc FTIR spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS) in order to characterize both the bulk and surface chemistry of the source material and printed samples. Scanning electron microscopy (SEM) and differential scanning calorimetry (DSC) were used to characterize morphology, cold crystallinity, and the glass transition and melting temperatures following printing. Analysis revealed calcium carbonate-based additives which were reacted with organic ligands and potentially trace metal impurities, both before and following printing. These additives became concentrated in voids in the printed structure. This finding is important for biomedical applications as carbonate will impact subsequent cell growth on printed tissue scaffolds. Results of chemical analysis also provided evidence of the hygroscopic nature of the source material and oxidation of the printed surface, and SEM imaging revealed micro-and submicron-scale roughness that will also impact potential applications.

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Section: Scanning Electron Microscopy (Sem) Resultsmentioning

confidence: 99%

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

et al. 2017

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“…12,13 Inspired by cicada wings, which are covered with nanopillar-shaped structures that are capable of killing bacteria through physical means rather than by using drug, scientists have discovered a way to reduce bacteria growth. [14][15][16][17][18][19][20][21][22] Specifically, studies have shown that when bacteria land on such nanopillar surfaces with features much smaller than the bacteria themselves, bacterial membranes are ruptured. 14,15 Therefore, an approach which relies on creating nanostructured surface features on the same material to be implanted may represent a novel nonpharmaceutical or non coating approach to reduce orthopedic implant infections.…”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17][18][19][20][21][22] More importantly, the tips of cicada wing nanocolumns were round and capped, which was in contrast to the tips of PEKK which were sharp-edged. Future studies will investigate the influence of the geometry of these PEKK nanoscale surface features and the resultant antibacterial response.…”

mentioning

confidence: 99%

Antibacterial properties of PEKK for orthopedic applications

Wang¹,

Bhardwaj²,

Webster³

2017

IJN

Self Cite

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Orthopedic implant infections have been steadily increasing while, at the same time, antibiotics developed to kill such bacteria have proven less and less effective with every passing day. It is clear that new approaches that do not rely on the use of antibiotics are needed to decrease medical device infections. Inspired by cicada wing surface topographical features, nanostructured surfaces represent a new approach for imposing antibacterial properties to biomaterials without using drugs. Moreover, new chemistries with altered surface energetics may decrease bacterial attachment and growth. In this study, a nanostructured surface was fabricated on poly-ether-ketone-ketone (PEKK), a new orthopedic implant chemistry, comprised of nanopillars with random interpillar spacing. Specifically, after 5 days, when compared to the orthopedic industry standard poly-ether-ether-ketone (PEEK), more than 37% less Staphylococcus epidermidis were found on the PEKK surface. Pseudomonas aeruginosa attachment and growth also decreased 28% after one day of culture, with around a 50% decrease after 5 days of culture when compared to PEEK. Such decreases in bacteria function were achieved without using antibiotics. In this manner, this study demonstrated for the first time, the promise that nanostructured PEKK has for numerous anti-infection orthopedic implant applications.

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“…During the last decades, researchers have contributed to widening the application range of PLA through chemical and physical alterations to the bulk and/or surface by introducing hydrophilic and biocompatible moieties [29,30]. Several modifications strategies such as plasma [31] or chemical [29] treatments have been employed to enable specific surface properties on PLA.…”

Section: Introductionmentioning

confidence: 99%

Engineered membranes for residual cell trapping on microfluidic blood plasma separation systems. A comparison between porous and nanofibrous membranes

Lopresti

Keraite

Ongaro

et al. 2020

Preprint

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Blood-based clinical diagnostics require challenging limit-of-detection for low abundance, circulating molecules in plasma. Micro-scale blood plasma separation (BPS) has achieved remarkable results in terms of plasma yield or purity, but rarely achieving both at the same time.Here, we proposed the first use of electrospun polylactic-acid (PLA) membranes as filters to remove residual cell population from continuous hydrodynamic-BPS devices. The membranes hydrophilicity was improved by adopting a wet chemistry approach via surface aminolysis as demonstrated through Fourier Transform Infrared Spectroscopy and Water Contact Angle analysis.The usability of PLA-membranes was assessed through degradation measurements at extreme pH values. Plasma purity and hemolysis were evaluated on plasma samples with residual red blood cell content (1, 3, 5% hematocrit) corresponding to output from existing hydrodynamic BPS systems.Commercially available membranes for BPS were used as benchmark. Results highlighted that the electrospun membranes are suitable for downstream residual cell removal from blood, permitting the collection of up to 2 mL of pure and low-hemolyzed plasma. Fluorometric DNA quantification revealed that electrospun membranes did not significantly affect the concentration of circulating DNA. PLA-based electrospun membranes can be combined with hydrodynamic BPS in order to achieve high volume plasma separation at over 99% plasma purity.

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Cold atmospheric plasma (CAP) surface nanomodified 3D printed polylactic acid (PLA) scaffolds for bone regeneration

Cited by 163 publications

References 26 publications

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Antibacterial properties of PEKK for orthopedic applications

Engineered membranes for residual cell trapping on microfluidic blood plasma separation systems. A comparison between porous and nanofibrous membranes

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