Cold-adaptive mechanism of psychrophilic bacteria in food and its application

Jin, Shuanggen; Wang, Yizhe; Zhao, Xihong

doi:10.1016/j.micpath.2022.105652

Cited by 19 publications

(2 citation statements)

References 73 publications

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“…We surveyed its genome and identified many genes putatively involved in cold adaptation ( Supplementary Table S9 ). It is generally believed that the mechanism of action of cold shock proteins (CSPs) is similar to that of an RNA molecular chaperone, combining with single-stranded ribonucleic acid/deoxyribonucleic acid and promoting the effective folding of ribonucleic acid/deoxyribonucleic acid, preventing protein misfolding, and ensuring the stability of ribonucleic acid and deoxyribonucleic acid secondary structures [ 54 ]. In the genome of strain MTB7, six CPSs were identified, of which one showed high similarity to that of Photobacterium profundum SS9, a model psychrophilic and piezophilic Gram-negative bacteria.…”

Section: Resultsmentioning

confidence: 99%

Genomic Analysis of the Deep-Sea Bacterium Shewanella sp. MTB7 Reveals Backgrounds Related to Its Deep-Sea Environment Adaptation

Wang

Liu

et al. 2023

Microorganisms

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Shewanella species are widely distributed in various environments, especially deep-sea sediments, due to their remarkable ability to utilize multiple electron receptors and versatile metabolic capabilities. In this study, a novel facultatively anaerobic, psychrophilic, and piezotolerant bacterium, Shewanella sp. MTB7, was isolated from the Mariana Trench at a depth of 5900 m. Here, we report its complete genome sequence and adaptation strategies for survival in deep-sea environments. MTB7 contains what is currently the third-largest genome among all isolated Shewanella strains and shows higher coding density than neighboring strains. Metabolically, MTB7 is predicted to utilize various carbon and nitrogen sources. D-amino acid utilization and HGT-derived purine-degrading genes could contribute to its oligotrophic adaptation. For respiration, the cytochrome o ubiquinol oxidase genes cyoABCDE, typically expressed at high oxygen concentrations, are missing. Conversely, a series of anaerobic respiratory genes are employed, including fumarate reductase, polysulfide reductase, trimethylamine-N-oxide reductase, crotonobetaine reductase, and Mtr subunits. The glycine reductase genes and the triplication of dimethyl sulfoxide reductase genes absent in neighboring strains could also help MTB7 survive in low-oxygen environments. Many genes encoding cold-shock proteins, glycine betaine transporters and biosynthetic enzymes, and reactive oxygen species-scavenging proteins could contribute to its low-temperature adaptation. The genomic analysis of MTB7 will deepen our understanding of microbial adaptation strategies in deep-sea environments.

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Section: Resultsmentioning

confidence: 99%

Genomic Analysis of the Deep-Sea Bacterium Shewanella sp. MTB7 Reveals Backgrounds Related to Its Deep-Sea Environment Adaptation

Wang

Liu

et al. 2023

Microorganisms

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show abstract

“…The structure can undergo conformational changes according to the needs of catalytic activity, making up for the lack of activation energy at low temperatures. 25 For example, there are phenol 2-monooxgenase, and enzymes involved in the ortho-cleavage of catechol were present in the aerobic strain AQ5-05. Cold-adapted enzymes C 12 O and C 23 O enzymes have been found in several cold-adapted phenol-degrading strains.…”

Section: Microorganisms Cold-adapted Mechanismmentioning

confidence: 99%