Cold‐Adapted Reassortants of Influenza A Virus in MDCK Cells

Odagiri, Takato; Smitka, Curt W.; Maassab, Hunein F.

doi:10.1111/j.1348-0421.1983.tb03574.x

Cited by 6 publications

(1 citation statement)

References 11 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the single-gene reassortants t h a t possessed only R N A 2 coding for the P B 2 gene or R N A 5 coding for the neuraminidase (NA) gene of A/AA/6/60 ca variant, were involved in the ca and non ts phenotypes. There reassortants allowed us to determine the function of the ca m u t a t i o n in viral attenuation in mice (24) as well as the biological mechanism t h a t operates to lower the viral replication range to 25 ° C.…”

Section: Introductionmentioning

confidence: 99%

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

Odagiri

Tosaka

Ishida

et al. 1986

Archives of Virology

View full text Add to dashboard Cite

The biological function of a cold-adapted (ca) mutation residing on the PB2 gene of an influenza A/Ann Arbor/6/60 (A/AA/6/60) ca variant virus in the viral replication cycle at 25 degrees C was studied. The viral polypeptide synthesis of A/AA/6/60 ca variant at 25 degrees C was evident approximately 6 hours earlier than the wild type (wt) virus and yielded twice as many products. The quantitative analysis of viral complementary RNA (cRNA), synthesized in the presence of cycloheximide, revealed that A/AA/6/60 ca variant and a single gene reassortant that contains only the PB2 gene of the ca variant with remaining genes of the wt virus produced equal amount of cRNA at 25 degrees and 33 degrees C, which was an amount approximately four fold greater than the wt virus' cRNA synthesized at 25 degrees C. These results strongly suggest that the ca mutation residing on the PB2 gene of A/AA/6/60 ca variant affects the messenger RNA synthesis at 25 degrees C in the primary transcription.

show abstract

Section: Introductionmentioning

confidence: 99%

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

Odagiri

Tosaka

Ishida

et al. 1986

Archives of Virology

View full text Add to dashboard Cite

show abstract

The Control of Influenza

Kilbourne

1987

Influenza

View full text Add to dashboard Cite

Cold-adapted reassortants of influenza A virus: Pathogenicity of A/Ann Arbor/6/60�A/Alaska/6/77 reassortant virusesIn vivo andIn vitro

Heath

Maassab

Odagiri

et al. 1986

Archives of Virology

View full text Add to dashboard Cite

Cold-adapted reassortants of A/Ann Arbor/6/60 X A/Alaska/6/77 viruses made in MDCK cells have recently been assessed genotypically and for temperature-sensitive and cold-adapted phenotypes. These reassortants were used to infect ferrets and hamsters and to inoculate organ cultures of hamster tracheal rings, in order to assess their degree of virulence. Virulence in the three model systems corresponded quite well, and a correlation between loss of virulence and particular A/AA/6/60 genes present in the reassortants was noted. Two different reassortants containing either RNA 2 or RNA 5 (NA gene) alone from A/AA/6/60 showed little attenuation from the wild-type parent. A reassortant containing both RNA 2 and the NA gene from A/AA/6/60 and all remaining wild-type genes showed some small decrease in virulence compared to the wild-type virus. However a reassortant containing these two A/AA/6/60 genes and RNA 3 as an additional gene from this parent, had a level of attenuation comparable to that of the cold-adapted virus.

show abstract

Cold‐Adapted Reassortants of Influenza A Virus in MDCK Cells

Cited by 6 publications

References 11 publications

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

The Control of Influenza

Cold-adapted reassortants of influenza A virus: Pathogenicity of A/Ann Arbor/6/60�A/Alaska/6/77 reassortant virusesIn vivo andIn vitro

Contact Info

Product

Resources

About