Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

Abstract

Cold-active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold-active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme production was achieved by optimization of medium and culture conditions. Maximum CAT was demonstrated under low glucose content (2%), 10% inoculum size, temperature 20°C, and dissolved oxygen concentration (DO) 40%. An effective laboratory technology based on changing the oxidative stress level through an increase of DO in the bioreactor was developed. The used strategy resulted in a 1.7-and 1.4-fold enhanced total enzyme activity and maximum enzyme productivity. The enzyme was purified and characterized. P. griseofulvum P29 CAT was most active at approximately 20°C and pH 6.0. Its thermostability was in the range between 5°C and 40°C.

A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2

“…Both antioxidant enzymes degrade • O2 − and H 2 O 2 , respectively, the generation of which is accelerated under stress conditions. Increased activity of antioxidant enzymatic defense is one of the main characteristics of stress caused by low temperatures [33,55]. ROS plays a role in cell signaling.…”

“…Cat-2 from the mesophilic Serratia marcescens SYBC-01 isolated from Wuxi City, China, exhibits significant relative activity (73.8%) at 0 • C, and its ideal temperature is roughly 20 • C. The authors characterized this enzyme as a type of cold-adaptive enzyme [30]. Krumova et al (2021) reported CA CAT produced by a psychrotolerant strain of P. griseofulvum [33]. The catalase studied was an intracellular enzyme.…”

“…There are few reports on CA catalase produced by Antarctic fungi [24]. The second published information is by Krumova et al (2021) on CA intracellular CAT produced by an Antarctic strain of P. griseofulvum p 29 [33]. The authors developed a laboratory technology for the production of the enzyme and purified and characterized the resulting enzyme.…”

Advances in cold-adapted enzymes derived from microorganisms

“…To date, cold-adapted oxidoreductases derived from microorganisms mainly consist of sorbitol dehydrogenase, glutathione reductase, hydrogen peroxide reductase, and superoxide dismutase. Of these, cold-adapted superoxide dismutase is commonly used in clinical debridement and for other therapeutic purposes ( DangThu et al, 2020 ; Wang Z. P. et al, 2020 ; Wang Q. et al, 2020 ; Krumova et al, 2021 ). Presently, Pseudoalteromonas , Salmonella , and the fungi Aspergillus are the primary sources of cold-adapted superoxide dismutases.…”