Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in
            <i>Caenorhabditis elegans</i>

Okahata, Misaki; Wei, Aguan; Ohta, Akane; Kuhara, Atsushi

doi:10.1126/sciadv.aav3631

Cited by 21 publications

(47 citation statements)

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“…2D of Ujisawa et al 12 , thermal-dependent Ca 2+ concentration changes in ADL upon a 6 °C range-warming stimuli in the osm-9 ocr-2; ocr-1 triple mutant revealed decreased thermal responses compared with wild-type 12 . Additionally, Okahata et al Figure S6 15 described that osm-9 and ocr-2 single mutants and the osm-9 ocr-2 double mutant exhibited normal phenotypes with regard to ADL thermal responses upon the same 6 °C range-warming 15 .…”

Section: Resultsmentioning

confidence: 99%

“…The survival rates of wild-type animals conditioned at [15 °C → 2 °C], [15 °C → 25 °C(3 h) → 2 °C], or [15 °C → 25 °C(5 h) → 2 °C] were approximately 86%, 46%, and 23%, respectively (Fig. 2 a–c) 5 , 10 , 15 . In contrast, osm-9 mutant animals exhibited abnormally elevated cold tolerance under the 15 °C → 25 °C(3 or 5 h) → 2 °C protocol (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…TRPV channels OSM-9, OCR-1, and OCR-2 act in temperature sensation by ADL (Fig. 1 b) 12 , 15 . Thermal-dependent Ca 2+ concentration changes in ADL upon warming stimuli in osm-9 ocr-2; ocr-1 triple mutant animals were decreased compared with wild-type ADL 12 .…”

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confidence: 99%

“…Thermal-dependent Ca 2+ concentration changes in ADL upon warming stimuli in osm-9 ocr-2; ocr-1 triple mutant animals were decreased compared with wild-type ADL 12 . Cultivation of OSM-9/OCR-1/OCR-2 mutants at 25 °C resulted in abnormal temperature acclimatisation and decreased ADL neural activity in response to temperature stimuli, suggesting that TRP channels are necessary for ADL temperature signalling for temperature acclimatisation 12 , 15 . However, it remains unclear whether these TRPs act as temperature receptors in ADL.…”

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confidence: 99%

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OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation

Ohnishi

Saito

Miura

et al. 2020

Sci Rep

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Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation

Ohnishi

Saito

Miura

et al. 2020

Sci Rep

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“…In addition to Songpu mirror carp and Barbless carp, organisms including zebra sh [33,34], Caenorhabditis elegans [44,45] and plants [46] possess a cold acclimation ability to allow their survival in extremely low temperature. In this study, many genes that are previously reported to associate with the cold acclimation of zebra sh [33] were found to be differentially expressed.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species

Liu

Ren

et al. 2020

Preprint

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Background Closely related species of the carp family ( Cyprinidae ) have evolved distinctive abilities to survive under cold stress, but molecular mechanisms underlying the generation of cold resistance remain largely unknow. In this study, we compared transcriptomic profiles of two carp species to identify key factors and pathways for cold tolerance and acclimation. Results Larvae of Songpu mirror carp and Barbless carp that were pretreated at 18°C for 24 hours significantly improved their survival rates under lethal cold temperature at 8°C or 10°C, indicating that two carp species possess the ability of cold acclimation. However, Songpu mirror carp exhibited stronger abilities of cold tolerance and acclimation than Barbless carp. Transcriptomic profiles of Songpu mirror carp and Barbless carp larvae at 28°C and 18°C were compared during cold acclimation through RNA-seq. Differentially expressed genes that are closely associated with the differences in cold acclimation between two carp species were identified through bioinformatics and Venn's diagram analysis. GO enrichment analysis of these genes indicated that cellular component assembly involved in morphogenesis, secondary alcohol metabolism and drug transport were the most up-regulated biological processes during cold acclimation of Songpu mirror carp. Conversely, positive regulation of macroautophagy, intracellular protein transport, and organonitrogen compound catabolism were the most down-regulated biological processes during cold acclimation of Barbless carp. KEGG enrichment analysis revealed that factors in the FoxO-related signaling pathways are mainly responsible for the development of differences in cold tolerance and acclimation between two carp species since altering the phosphorylation of key proteins in the FoxO-related signaling pathways with inhibitors or an activator significantly decreased the cold tolerance and acclimation of Songpu mirror carp. These data provided key clues for dissection of molecular mechanisms underlying the development of cold tolerance and acclimation in carps. Conclusions These findings indicate that larvae of two carp species possess different abilities of cold tolerance and can build cold acclimation under mild low temperature. Multiple biological processes and FoxO-related signaling pathways are closely associated with the development of differences in cold tolerance and acclimation between two carp species.

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Temperature sensing and context-dependent thermal behavior in nematodes

Glauser

2022

Current Opinion in Neurobiology

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Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans

Abstract: Environmental oxygen concentration affects cold acclimation via KQT potassium channel in the nematode Caenorhabditis elegans.

Cited by 21 publications

References 39 publications

OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation

OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation

Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species

Temperature sensing and context-dependent thermal behavior in nematodes

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