Colchicine treatment in the preparation of varicella-zoster virus inocula

Cole, Nancy L.; Kingsley, Robert E.

doi:10.1016/0166-0934(92)90162-7

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…Because the results in the previous experiments represented a new application of confocal microscopy, we turned to a traditional method to confirm the differences in VZV cell-to-cell spread, namely, infectious center assays (Cole and Kingsley, 1992;Grose and Brunell, 1978). For these titrations, the initial virus inocula were replicate samples of VZV-infected cells frozen and stored in liquid nitrogen.…”

Section: Vzv Infectious Center Assaysmentioning

confidence: 99%

“…Infectious center assays of VZV-MSP and VZV-32. Infectious center assays were carried out by previously described methods (Cole and Kingsley, 1992;Grose and Brunell, 1978). The numbers of infectious centers present at 24 and 48 h postinfection were determined for VZV-MSP and VZV-32.…”

Section: Growth Of Vzv-msp In the Scid-hu Mousementioning

confidence: 99%

“…Transfer of infectivity was carried out by inoculation of trypsin-dispersed infected cells onto an uninfected monolayer at a 1:8 ratio of infected:uninfected cells unless otherwise noted (Grose and Brunell, 1978). Similarly, infectious center assays were carried out by described methods; these assays included both melanoma cell and human neonatal foreskin cell substrates (Cole and Kingsley, 1992;Grose and Brunell, 1978).…”

Section: Cells Viruses and Transfer Of Infectivitymentioning

confidence: 99%

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Varicella-Zoster Virus gE Escape Mutant VZV-MSP Exhibits an Accelerated Cell-to-Cell Spread Phenotype in both Infected Cell Cultures and SCID-hu Mice

et al. 2000

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Varicella-zoster virus is considered to have one of the most stable genomes of all human herpesviruses. In 1998, we reported the unanticipated discovery of a wild-type virus that had lost an immunodominant B-cell epitope on the gE ectodomain (VZV-MSP); the gE escape mutant virus exhibited an unusual pattern of egress. Further studies have now documented a markedly enhanced cell-to-cell spread by the mutant virus in cell culture. This property was investigated by laser scanning confocal microscopy combined with a software program that allows the measurement of pixel intensity of the fluorescent signal. For this new application of imaging technology, the VZV immediate early protein 62 (IE 62) was selected as the fluoresceinated marker. By 48 h postinfection, the number of IE 62-positive pixels in the VZV-MSP-infected culture was nearly fourfold greater than the number of pixels in a culture infected with a low-passage laboratory strain. Titrations by infectious center assays supported the above image analysis data. Confirmatory studies in the SCID-hu mouse documented that VZV-MSP spread more rapidly than other VZV strains in human fetal skin implants. Generally, the cytopathology and vesicle formation produced by other strains at 21 days postinfection were demonstrable with VZV-MSP at 14 days. To assess whether additional genes were contributing to the unusual VZV-MSP phenotype, approximately 20 kb of the VZV-MSP genome was sequenced, including ORFs 31 (gB), 37 (gH), 47, 60 (gL), 61, 62 (IE 62), 66, 67 (gI), and 68 (gE). Except for a few polymorphisms, as well as the previously discovered mutation within gE, the nucleotide sequences within most open reading frames were identical to the prototype VZV-Dumas strain. In short, VZV-MSP represents a novel variant virus with a distinguishable phenotype demonstrable in both infected cell cultures and SCID-hu mice.

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Section: Vzv Infectious Center Assaysmentioning

confidence: 99%

Section: Growth Of Vzv-msp In the Scid-hu Mousementioning

confidence: 99%

Section: Cells Viruses and Transfer Of Infectivitymentioning

confidence: 99%

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Varicella-Zoster Virus gE Escape Mutant VZV-MSP Exhibits an Accelerated Cell-to-Cell Spread Phenotype in both Infected Cell Cultures and SCID-hu Mice

et al. 2000

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Membrane fusion mediated by herpesvirus glycoproteins: the paradigm of varicella‐zoster virus

Cole

Grose

2003

Reviews in Medical Virology

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Varicella-zoster virus (VZV) is well known for its propensity to cause polykaryons (syncytia) in the vesicles within infected skin. Similarly in cultured cells, VZV induces extensive syncytial formation by virus-mediated cell-to-cell fusion. Statistical analyses of fusion parameters demonstrated three-way interactive effects among all three tested variables (incubation temperature, cell type and virus strain). For example, fusion was greatly enhanced at 33 degrees C vs 37 degrees C; also fusion was pronounced in epidermal cells but negligible in fibroblast cells. As with all herpesviruses, VZV gH was a major fusogen. VZV cell fusion was inhibited by antibody to gH, but surprisingly was enhanced by antibody to gE. Other evidence implicating a role for VZV gE in the fusion process was provided by two mutant viruses, in which gE cell surface expression was enhanced. Under transfection conditions, VZV fusion formation occurred after expression of the gH/gL complex; in contrast, pseudorabies virus requires expression of gH, gL and gB, while the herpes simplex virus (HSV) types 1 and 2 require the quartet of gH, gL, gB and gD. VZV has no gD gene and no apparent gD functional homologue. On the other hand, VZV gE exerts a greater effect than HSV gE on membrane fusion. Taken together, the data in this review suggest that VZV has evolved viral glycoprotein machinery more geared toward cell-to-cell fusion (fusion-from-within) than toward virus-to-cell fusion (entry/fusion-from-without), as a means for syncytium formation within the human epidermis.

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A systematic review of the infectious complications of colchicine and the use of colchicine to treat infections

McEwan

Robinson

2021

Seminars in Arthritis and Rheumatism

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Colchicine treatment in the preparation of varicella-zoster virus inocula

Cited by 3 publications

References 7 publications

Varicella-Zoster Virus gE Escape Mutant VZV-MSP Exhibits an Accelerated Cell-to-Cell Spread Phenotype in both Infected Cell Cultures and SCID-hu Mice

Varicella-Zoster Virus gE Escape Mutant VZV-MSP Exhibits an Accelerated Cell-to-Cell Spread Phenotype in both Infected Cell Cultures and SCID-hu Mice

Membrane fusion mediated by herpesvirus glycoproteins: the paradigm of varicella‐zoster virus

A systematic review of the infectious complications of colchicine and the use of colchicine to treat infections

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