Colchicine induces apoptosis in organotypic hippocampal slice cultures

Kristensen, Bjarne Winther; Noer, Helle; Gramsbergen, Jan Bert; Zimmer, Jens; Noraberg, Jens

doi:10.1016/s0006-8993(02)04080-5

Cited by 70 publications

(47 citation statements)

References 72 publications

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“…34 When colchicin, being a strong microtubule-depolymerizing agent, is implemented intracranially in experimental studies, it causes deficits in memory 35 which is thought to be related by inducing apoptosis. 36 Therefore, it is used as a selective neurotoxin in the animal models for Alzheimer disease and epilepsy. 35 However, in its usage in humans, it is reported that its absorption is rapid in oral use and presenting in high concentrations in kidney, liver and spleen whereas it is not absorbed by heart, skeletal muscle and brain.…”

Section: Discussionmentioning

confidence: 99%

Executive dysfunctions and depression in Behçet’s disease without explicit neurological involvement

Erberk-Özen¹,

Bırol

Boratav³

et al. 2006

Psychiatry Clin Neurosci

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This study aims to assess the executive functions and depression status in patients with Behçet's disease without explicit neurological involvement and to evaluate cognitive functions in this group of patients independent of accompanying depression. In the present study, 30 patients with Behçet's disease in the non-active phase of their illness and 30 healthy volunteers were included. In the evaluation of depression levels, Beck Depression Inventory was employed. The executive functions of the patients were evaluated by Wisconsin Card Sorting Test (WCST) and Stroop Test (ST). Beck Depression Inventory scores measured in the Behçet's disease group, in which no clinically serious depression was observed, were higher than the control group. Then, it was observed that the scores of neuropsychological tests of the Behçet's disease group were found to be significantly lower, especially in WCST, while significant relations were revealed between Beck Depression Inventory scores and WCST, ST scores. The authors' findings suggest that there may be factors other than depression causing executive impairment in patients with Behçet's disease without explicit neurological symptoms. Executive dysfunctions may occur in association with mild depressive states due to chronic disease stress or silent and/or future neurological involvement of Behçet's disease, especially in the frontal lobes.

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Section: Discussionmentioning

confidence: 99%

Executive dysfunctions and depression in Behçet’s disease without explicit neurological involvement

Erberk-Özen¹,

Bırol

Boratav³

et al. 2006

Psychiatry Clin Neurosci

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“…As a control, we assessed Ac-DEVD-AMC cleavage following treatment with colchicine (10 M). Colchicine inhibits microtubule polymerization and has been shown to induce apoptosis in cerebral granular neurons (5) and organotypic slices (33). We observed no significant difference in caspase 3-like protease activities between WT and puma Ϫ/Ϫ neuron cultures following colchicine treatment (Fig.…”

Section: Resultsmentioning

confidence: 58%

Characterization of Puma-Dependent and Puma-Independent Neuronal Cell Death Pathways following Prolonged Proteasomal Inhibition

Tuffy

Concannon

D'Orsi

et al. 2010

Molecular and Cellular Biology

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Proteasomal stress and the accumulation of polyubiquitinated proteins are key features of numerous neurodegenerative disorders. Previously we demonstrated that stabilization of p53 and activation of its target gene, puma (p53-upregulated mediator of apoptosis), mediated proteasome inhibitor-induced apoptosis in cancer cells. Here we demonstrated that Puma also contributed to proteasome inhibitor-induced apoptosis in mouse neocortical neurons. Although protection afforded by puma gene deletion was incomplete, we found little evidence indicating contributions from other proapoptotic BH3-only proteins. Attenuation of bax expression did not further reduce Puma-independent apoptosis, suggesting that pathways other than the mitochondrial apoptosis pathway were activated. Real-time imaging experiments in wild-type and puma-deficient neurons using a fluorescence resonance energy transfer (FRET)-based caspase sensor confirmed the involvement of a second cell death pathway characterized by caspase activation prior to mitochondrial permeabilization and, more prominently, a third, caspase-independent and Puma-independent pathway characterized by rapid cell shrinkage and nuclear condensation. This pathway involved lysosomal permeabilization in the absence of autophagy activation and was sensitive to cathepsin but not autophagy inhibition. Our data demonstrate that proteasomal stress activates distinct cell death pathways in neurons, leading to both caspase-dependent and caspase-independent apoptosis, and demonstrate independent roles for Puma and lysosomal permeabilization in this model.

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“…This study also offers the first indication that the self-aggregation CaMKIIα is dependent on microtubule stability, and it introduces p600 as the mediator of this effect. Since Ca 2+ overload, microtubule instability and p600 depletion have all been independently shown to cause neuronal death [17,34,35], this study puts us closer to a unified model wherein p600 is an interface between microtubule dynamics, Ca 2+ signalling, and neuronal survival.…”

Section: Discussionmentioning

confidence: 79%

p600 stabilizes microtubules to prevent the aggregation of CaMKIIα during photoconductive stimulation

Belzil¹,

Ramos²,

Sanada³

et al. 2014

Cellular and Molecular Biology Letters

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Colchicine induces apoptosis in organotypic hippocampal slice cultures

Cited by 70 publications

References 72 publications

Executive dysfunctions and depression in Behçet’s disease without explicit neurological involvement

Executive dysfunctions and depression in Behçet’s disease without explicit neurological involvement

Characterization of Puma-Dependent and Puma-Independent Neuronal Cell Death Pathways following Prolonged Proteasomal Inhibition

p600 stabilizes microtubules to prevent the aggregation of CaMKIIα during photoconductive stimulation

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