COL1A1 promotes metastasis in colorectal cancer by regulating the WNT/PCP pathway

Abstract

Colorectal cancer (CRC) is the third leading cause of cancer-associated mortality, and is a major health problem. Collagen type I α 1 (COL1A1) is a major component of collagen type I. Recently, it was reported to be overexpressed in a variety of tumor tissues and cells. However, the function of COL1A1 in CRC remains unclear. Herein, the present study demonstrated that COL1A1 was upregulated in CRC tissues and the paired lymph node tissues. Transwell assays showed that COL1A1 promoted CRC cell migration in vitro. Moreover, it was revealed that COL1A1 levels were correlated with those of WNT/planar cell polarity (PCP) signaling pathway genes; inhibition of COL1A1 decreased the expression levels of Ras-related C3 botulinum toxin substrate 1-GTP, phosphorylated-c-Jun N-terminal kinase, and RhoA-GTP, all of which are key genes in the WNT/PCP signaling pathway. These results may indicate the mechanisms underlying the oncogenic role of COL1A1 in CRC. In summary, the present data indicated that COL1A1 may serve as an oncoprotein, and that it may be used as a potential therapeutic target in CRC.

Construction of a lncRNA-Associated ceRNA Network and Identification of A Potential Six-lncRNA Prognostic Signature for Esophageal Cancer

“…In the EC ceRNA network, sponging of overexpressed lncRNA AC009093.1 and under expressed lncRNA MAGI2-AS3, relieved their targets COL1A1 and COL5A2 from being suppressed by hsa-mir-143. Previous studies have suggested that hsa-mir-143 functions as a tumor suppressor in several cancer types [36,37], and COL1A1 has been shown to act as an oncogene to regulate tumors [38][39][40]. Our ndings implied that hsa-mir-143 may function as a tumor suppressor by targeting COL1A1 and COL5A2 in EC and competing with lncRNA MAGI2-AS3/AC009093.1.…”

A lncRNA-SWI/SNF complex crosstalk controls transcriptional activation at specific promoter regions

“…6a and Supplementary Fig. 9A) [43][44][45] . The transcriptional activation of these genes is highly dependent on SWINGN expression, as we demonstrated by upregulating its levels by CRISPR activation (CRISPRa) in H226 lung cancer cells.…”

Construction of a lncRNA-associated ceRNA network and identification of a potential six-lncRNA prognostic signature for esophageal cancer

“…In the EC ceRNA network, sponging of overexpressed lncRNA AC009093.1 and under expressed lncRNA MAGI2-AS3, relieved their targets COL1A1 and COL5A2 from being suppressed by hsa-mir-143. Previous studies have suggested that hsa-mir-143 functions as a tumor suppressor in several cancer types [36,37], and COL1A1 has been shown to act as an oncogene to regulate tumors [38][39][40].…”