Coinfection with<i>Schistosoma mansoni</i>Is Associated with Decreased HIV-Specific Cytolysis and Increased IL-10 Production

McElroy, Michael D; Elrefaei, Mohamed; Jones, Norman; Ssali, Francis; Mugyenyi, Peter; Barugahare, Banson; Cao, Huyen

doi:10.4049/jimmunol.174.8.5119

Cited by 43 publications

(37 citation statements)

References 37 publications

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“…Few studies have reported the relationship between cytokines in individuals with HIV and parasitic intestinal infections. Some authors evaluated cytokines and their relationship with parasites such as Schistosoma mansoni, Wuchereria bancrofti, and Trichinella spiralis (71)(72)(73)(74)(75)(76)(77)(78)(79). No correlation between serum INF-γ, IL-2, and IL-10 levels and intestinal parasitic infections was seen.…”

Section: Discussionmentioning

confidence: 99%

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects

Amâncio¹,

Pascotto²,

Souza³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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This study applied a socioeconomic questionnaire designed to evaluate the frequency of intestinal parasites and characterize epidemiological, nutritional, and immunological variables in 105 HIV/AIDS patients -with and without parasitic infections, attending the Day Hospital in Botucatu, UNESP, from 2007 to 2008. Body mass index was calculated and the following tests performed: parasitological stool examinations; eosinophil, IgE, CD4 + T and CD8 + T lymphocyte cell counts; albumin test; viral load measure; and TNF-α, IFN-γ, IL-2, IL-5 and IL-10 cytokine levels. Results were positive for parasitic intestinal infections in 12.4% of individuals. Most patients had good socioeconomic conditions with basic sanitation, urban dwellings, treated water supply and sewage, good nutritional and immunological status and were undergoing HAART. Parasites were found at the following frequencies: Entamoeba -five patients (38.5%), Giardia lamblia -four (30.7%), Blastocystis hominis -three (23.0%), Endolimax nana -two (15.4%), and Ascaris lumbricoides -one (7.7%). There were no significant differences between the two groups for eosinophils, albumin, IgE, CD4 + T and CD8 + T lymphocytes, INF-γ, IL-2, or IL-10. Most patients also showed undetectable viral load levels. Significant differences were found for TNF-α and IL-5. These results show the importance of new studies on immunodeficient individuals to increase understanding of such variables.

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Section: Discussionmentioning

confidence: 99%

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects

Amâncio¹,

Pascotto²,

Souza³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The detection of Gag-specific IL-10 production was performed using PBMC (1 ϫ 10 6 ) incubated with Gag peptide pools for 2 h at 37°C in 5% CO 2 in the presence of costimulatory anti-CD49d and anti-CD28 (1 g/ml; BD Biosciences) followed by GolgiStop (BD Pharmingen) for 12-14 h as previously described to be the optimal incubation period for the detection of IL-10 without affecting the viability of the cells (25). LPS (1 ng/ml; Sigma-Aldrich) and medium alone without Ag stimulation were used as positive and negative controls, respectively.…”

Section: Flow-based Intracellular Il-10 Stainingmentioning

confidence: 99%

HIV-Specific IL-10-Positive CD8+ T Cells Suppress Cytolysis and IL-2 Production by CD8+ T Cells

Elrefaei

Ventura

Baker

et al. 2007

The Journal of Immunology

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IL-10 producing T cells inhibit Ag-specific CD8+ T cell responses and may play a role in the immune dysregulation observed in HIV infection. We have previously observed the presence of HIV-specific IL-10-positive CD8+ T cells in advanced HIV disease. In this study, we examined the suppressive function of the Gag-specific IL-10-positive CD8+ T cells. Removal of these IL-10-positive CD8+ T cells resulted in increased cytolysis and IL-2, but not IFN-γ, production by both HIV- and human CMV-specific CD8+ T cells. In addition, these IL-10-positive CD8+ T cells mediated suppression through direct cell-cell contact, and had a distinct immunophenotypic profile compared with other regulatory T cells. We describe a new suppressor CD8+ T cell population in advanced HIV infection that may contribute to the immune dysfunction observed in HIV infection.

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“…We have previously demonstrated that S. mansoni coinfection was associated with increased frequency of IL-10-positive HIV-specific CD8 ϩ T cell responses (25). Therefore, only individuals without serologic evidence of S. mansoni infection were evaluated in the current study to exclude schistosomiasis as a contributing factor in HIV-specific IL-10 production.…”

Section: S Mansoni Elisamentioning

confidence: 99%

“…We first determined the frequency of Gag-specific IL-10-positive CD8 ϩ T cells in 61 ARV naive HIV-1-infected volunteers (25). Degranulation and IFN-␥ production by Gag-specific CD8 ϩ T cells were also determined concurrently.…”

Section: An Inverse Correlation Between Cd4mentioning

confidence: 99%

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HIV-Specific IL-10-Positive CD8+ T Cells Are Increased in Advanced Disease and Are Associated with Decreased HIV-Specific Cytolysis

Elrefaei

Barugahare

Ssali

et al. 2006

The Journal of Immunology

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IL-10-producing T cells have been shown to inhibit Ag-specific CD8+ T cell responses, and may play a role in the immune dysregulation observed in HIV-1 infection. We characterized the Gag-specific IL-10 responses by CD8+ T cells in HIV-1-positive volunteers from Uganda. HIV-specific IL-10 responses were detected in 32 of 61 (52.4%) antiretroviral naive and 2 of 15 (13.3%) volunteers with a complete virologic response on antiretroviral therapy (< 400 copies/ml). The frequency of HIV-specific IL-10-positive cells was significantly higher in volunteers with advanced disease (CD4+ T cell count <200 cells/mm3; p = 0.0004), and correlated positively with plasma HIV RNA (r = 0.43, p = 0.0004). Interestingly, the frequency of Gag-specific CD107a/b-, but not IFN-γ-, positive cells was significantly lower in individuals with detectable IL-10-positive CD8+ T cells (p = 0.004). Gag-specific IL-10-positive CD8+ T cells demonstrated a pattern of surface memory marker expression that is distinct compared with CD107a/b- and IFN-γ-positive CD8+ T cell populations (p < 0.0001). Our study describes a distinct population of IL-10-positive CD8+ T cells that may play a role in HIV-associated immune dysfunction.

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Coinfection withSchistosoma mansoniIs Associated with Decreased HIV-Specific Cytolysis and Increased IL-10 Production

Cited by 43 publications

References 37 publications

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects

HIV-Specific IL-10-Positive CD8+ T Cells Suppress Cytolysis and IL-2 Production by CD8+ T Cells

HIV-Specific IL-10-Positive CD8+ T Cells Are Increased in Advanced Disease and Are Associated with Decreased HIV-Specific Cytolysis

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