Coimmunoprecipitation of reversibly glycosylated polypeptide with sucrose synthase from developing castor oilseeds

Fedosejevs, Eric T.; Liu, Leo N.C.; Abergel, Megan; She, Yi‐Min; Plaxton, William C.

doi:10.1002/1873-3468.12893

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Finally, it is worth mentioning that even though Langeveld et al. (2002) found no other protein to be part of RGP containing complexes from wheat, UAM has been found in co-immunoprecipitations of sucrose synthase from various seeds and flowers (Fedosejevs et al. 2017) and in a xylan synthase complex from wheat (Zeng et al.…”

Section: Biological Importance Of Uamsmentioning

confidence: 99%

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Molecular characteristics of plant UDP-arabinopyranose mutases

et al. 2019

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l-arabinofuranose is a ubiquitous component of the cell wall and various natural products in plants, where it is synthesized from cytosolic UDP-arabinopyranose (UDP-Arap). The biosynthetic machinery long remained enigmatic in terms of responsible enzymes and subcellular localization. With the discovery of UDP-Arap mutase in plant cytosol, the demonstration of its role in cell-wall arabinose incorporation and the identification of UDP-arabinofuranose transporters in the Golgi membrane, it is clear that the cytosolic UDP-Arap mutases are the key enzymes converting UDP-Arap to UDP-arabinofuranose for cell wall and natural product biosynthesis. This has recently been confirmed by several genotype/phenotype studies. In contrast to the solid evidence pertaining to UDP-Arap mutase function in vivo, the molecular features, including enzymatic mechanism and oligomeric state, remain unknown. However, these enzymes belong to the small family of proteins originally identified as reversibly glycosylated polypeptides (RGPs), which has been studied for >20 years. Here, we review the UDP-Arap mutase and RGP literature together, to summarize and systemize reported molecular characteristics and relations to other proteins.

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Section: Biological Importance Of Uamsmentioning

confidence: 99%

“…2013) and Hordeum vulgare (Hsieh et al. 2016), and sequence analysis suggests a kingdom-wide distribution (Fedosejevs et al. 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristics of plant UDP-arabinopyranose mutases

et al. 2019

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“…Reversibly glycosylated polypeptides are found in numerous plant species, both dicots and monocots, as well as in algae, mosses, spore plants, etc. [ 6 , 10 , 37 ]. They play an important role at various stages of plant growth and development, contributing to cell wall formation, pollen development, response to different stresses, plant–pathogen interactions, and intercellular transport regulation.…”

Section: Discussionmentioning

confidence: 99%

“…They play an important role at various stages of plant growth and development, contributing to cell wall formation, pollen development, response to different stresses, plant–pathogen interactions, and intercellular transport regulation. Attention to this protein family is related mainly to their UDP-Arap mutase activity, as Arap–Araf interconversion is indispensable for plant development [ 6 , 10 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Nicotiana benthamiana Class 1 Reversibly Glycosylated Polypeptides Suppress Tobacco Mosaic Virus Infection

Kamarova,

Ershova,

Sheshukova

et al. 2023

IJMS

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Reversibly glycosylated polypeptides (RGPs) have been identified in many plant species and play an important role in cell wall formation, intercellular transport regulation, and plant–virus interactions. Most plants have several RGP genes with different expression patterns depending on the organ and developmental stage. Here, we report on four members of the RGP family in N. benthamiana. Based on a homology search, NbRGP1-3 and NbRGP5 were assigned to the class 1 and class 2 RGPs, respectively. We demonstrated that NbRGP1–3 and 5 mRNA accumulation increases significantly in response to tobacco mosaic virus (TMV) infection. Moreover, all identified class 1 NbRGPs (as distinct from NbRGP5) suppress TMV intercellular transport and replication in N. benthamiana. Elevated expression of NbRGP1–2 led to the stimulation of callose deposition at plasmodesmata, indicating that RGP-mediated TMV local spread could be affected via a callose-dependent mechanism. It was also demonstrated that NbRGP1 interacts with TMV movement protein (MP) in vitro and in vivo. Therefore, class 1 NbRGP1–2 play an antiviral role by impeding intercellular transport of the virus by affecting plasmodesmata callose and directly interacting with TMV MP, resulting in the reduced viral spread and replication.

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“…The UAM protein is widely distributed in plants (Drakakaki et al, 2006; Fedosejevs et al, 2017; Hsieh et al, 2016; Huang, He, Pei, et al, 2022; Huang, He, Zhao, et al, 2022; Konishi et al, 2007; Langeveld et al, 2002; Rancour et al, 2015; Rautengarten et al, 2011; Willis et al, 2016). However, the role of UAM protein in plant disease resistance has not been widely studied.…”

Section: Discussionmentioning

confidence: 99%

TaUAM3, a UDP‐Ara mutases protein, positively regulates wheat resistance to the stripe rust fungus

Huang

Bai

Qian

et al. 2023

Food and Energy Security

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Plant cell walls are composed of polysaccharides such as cellulose, hemicelluloses, and pectins, whose location and function differ depending on plant type. UDP‐Ara mutases (UAMs) have been reported to play important roles in plant development and response to various plant stresses (abiotic and biotic). However, little work has been reported on UAM in wheat. In this study, we dissected the role of the UAM family member, UAM3, during the interaction between wheat and the stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), and in response to treatment with salicylic acid (SA). RNA interference (RNAi)‐based stable silencing of TaUAM3 resulted in decreased resistance to Pst fungus. In addition, CRISPR‐mediated genome editing (GE) of TaUAM3 enhanced the susceptibility of wheat to Pst or compromised disease resistance accompanied by increased fungal growth and decreased H2O2 production in plant tissues. Moreover, the transcript levels of pathogenesis‐related (PR) genes and reactive oxygen species (ROS)‐generating genes were down‐regulated in both the RNAi‐silenced and CRISPR‐edited plants, while the ROS‐scavenging gene, TaCAT3, was up‐regulated. Therefore, TaUAM3 positively regulates the resistance of wheat to Pst.

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Coimmunoprecipitation of reversibly glycosylated polypeptide with sucrose synthase from developing castor oilseeds

Cited by 6 publications

References 29 publications

Molecular characteristics of plant UDP-arabinopyranose mutases

Molecular characteristics of plant UDP-arabinopyranose mutases

Nicotiana benthamiana Class 1 Reversibly Glycosylated Polypeptides Suppress Tobacco Mosaic Virus Infection

TaUAM3, a UDP‐Ara mutases protein, positively regulates wheat resistance to the stripe rust fungus

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