Coimmobilized whole cells of Pseudomonas reptilivora and Micrococcus glutamicus in calcium alginate gel for the production of L-glutamic acid

Sunitha, Iruvanti; Rao, K. Jagannadha; Ayyanna, C.

doi:10.1007/pl00008974

Cited by 4 publications

(5 citation statements)

References 5 publications

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“…Hence, alginate beads prepared by using 0.25 M calcium chloride were used in subsequent analysis. The optimum concentration of calcium chloride was similar to those obtained for the production of L-glutamic acid (Sunitha et al, 1998). Figure 2e illustrates the effect of different cell concentrations on β-glucosidase production.…”

Section: Optimization Of β-Glucosidase Productionsupporting

confidence: 71%

Characteristics of β-glucosidase production by Paecilomyces variotii and its potential application in bioassay system for boric acid determination

Ang¹,

Salleh

Bakar³

et al. 2012

Afr. J. Biotechnol.

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This study reports the potential application of Paecilomyces variotii immobilized in calcium alginate beads as a sensing element in the analysis of boric acid. In the presence of boric acid, β-glucosidase production of P. variotii was inhibited and the changes of β-glucosidase concentration were correlated to the concentrations of boric acid. The optimum conditions of β-glucosidase production were observed when 6% (w/v) mycelia of P. variotii was immobilized in 2% (w/v) sodium alginate with 0.25 M calcium chloride, at initial pH 7 and temperature 45°C. The response time of less than 3 h exhibited by P. variotii towards boric acid was observed. A linear response range of boric acid concentration was obtained within the range of 0.05 to 0.215% (w/v), with a detection limit of 0.037% (w/v). The immobilized cell beads were considered reproducible with the relative standard deviation (RSD) of 4.96 and 4.81% in the presence (0.2% w/v) and absence of boric acid, respectively. From the results, P. variotii could be beneficially useful towards developing an alternative approach for boric acid analysis.

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Section: Optimization Of β-Glucosidase Productionsupporting

confidence: 71%

Characteristics of β-glucosidase production by Paecilomyces variotii and its potential application in bioassay system for boric acid determination

Ang¹,

Salleh

Bakar³

et al. 2012

Afr. J. Biotechnol.

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“…As mentioned previously, we found two volatile organic compounds (beta‐myrcene and benzothiazole) enriched in the root of ancient bayberrys, and we speculated that the enrichment of these compounds increased the genera Bacillus and was induced by biotic or abiotic stress. Furthermore, Pseudomonas could produce L‐glutamic acid, which is involved in a number of metabolic activities in plants (Sunitha et al, 1997), as suggested by our results from BIO‐Sankey (Figure 10). A recent study by Patil et al (2019) reported that eight different Pseudomonas species have the ability to release L‐glutamic acid with a biostimulatory effect, which can be employed to stimulate plant development.…”

Section: Discussionsupporting

confidence: 59%

“…Furthermore, Pseudomonas could produce L-glutamic acid, which is involved in a number of metabolic activities in plants (Sunitha et al, 1997), as suggested by our results from BIO-Sankey (Figure 10).…”

Section: Discussionmentioning

confidence: 59%

Ancient bayberry increased stress resistance by enriching tissue‐specific microbiome and metabolites

Li,

Wang,

Ren

et al. 2024

Physiologia Plantarum

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The ancient bayberry demonstrates superior resistance to both biotic and abiotic stresses compared to cultivated bayberry, yet the underlying mechanisms remain largely unexplored. This study investigates whether long‐term bayberry cultivation enhances stress resistance through modulation of tissue‐specific microbes and metabolites. Employing microbiome amplicon sequencing alongside untargeted mass spectrometry analysis, we scrutinize the role of endosphere and rhizosphere microbial communities and metabolites in shaping the differential resistance observed between ancient and cultivated bayberry trees. Our findings highlight the presence of core microbiome and metabolites across various bayberry tissues, suggesting that the heightened resistance of ancient bayberry may stem from alterations in rhizosphere and endosphere microbial communities and secondary metabolites. Specifically, enrichment of Bacillus in roots and stems, Pseudomonas in leaves, and Mortierella in rhizosphere soil of ancient bayberry was noted. Furthermore, correlation analysis underscores the significance of enriched microbial species in enhancing ancient bayberry's resistance to stresses, with elevated levels of resistance‐associated metabolites such as beta‐myrcene, benzothiazole, L‐glutamic acid, and gamma‐aminobutyric acid identified through GC–MS metabolomics analysis. The beneficial role of these resistance‐associated metabolites was further elucidated through assessment of their promotive and allelopathic effects, as well as their phytostatic and antioxidant functions in lettuce plants. Ultimately, our study delves into the intrinsic reasons behind the greater resistance of ancient bayberry to biotic and abiotic stresses by evaluating the impact of long‐term planting on the microbial community and metabolites in the bayberry endosphere and rhizosphere, shedding light on the complex dynamics of host‐microbial interactions.

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“…1982), a lipase activity of potential commercial value (Lanser et al. 2002) and the commercially valuable glutamic acid when co‐immobilized in calcium alginate beads with Micrococcus glutamicus (Sunitha et al. 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The bacterium Pseudomonas reptilivora has been shown to be pathogenic for reptiles and fish (Caldwell and Ryerson 1940;Li et al 1978). This micro-organism has also been found to produce three antibiotics (Del Rio et al 1972;Martinez-Molina and Olivares 1979;Martinez-Molina et al 1982), a lipase activity of potential commercial value (Lanser et al 2002) and the commercially valuable glutamic acid when co-immobilized in calcium alginate beads with Micrococcus glutamicus (Sunitha et al 1998). Despite its pathogenicity and its usefulness in commercial applications, little is known about its nucleic acid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Regulation of pyrimidine nucleotide formation in Pseudomonas reptilivora

West¹

2004

Lett Appl Microbiol

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Aims: To study the regulation of de novo pyrimidine biosynthesis in the pathogenic bacterium Pseudomonas reptilivora ATCC 14836. Methods and Results: The pyrimidine biosynthetic pathway enzymes were assayed in extracts of Ps. reptilivora ATCC 14836 cells and of cells from an auxotroph lacking aspartate transcarbamoylase activity. Pyrimidine biosynthetic pathway enzyme activities in ATCC 14836 were influenced by the addition of pyrimidine bases to the culture medium with orotic acid addition inducing dihydroorotase activity. Pyrimidine starvation of the transcarbamoylase mutant strain increased its de novo enzyme activities suggesting that the de novo pathway was also subject to repression by a pyrimidine-related compound. Aspartate transcarbamoylase activity in ATCC 14836 was inhibited in vitro by pyrophosphate and ATP. Conclusions: Regulation of pyrimidine biosynthesis in Ps. reptilivora was observed at the level of enzyme synthesis and at the level of activity for aspartate transcarbamoylase. Its regulation of enzyme synthesis seemed to be more highly controlled than what was observed in the related species Ps. fluorescens. Significance and Impact of the Study: This investigation found that pyrimidine biosynthesis is controlled in Ps. reptilivora. This could prove helpful to future studies exploring its pathogenicity.

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Coimmobilized whole cells of Pseudomonas reptilivora and Micrococcus glutamicus in calcium alginate gel for the production of L-glutamic acid

Cited by 4 publications

References 5 publications

Characteristics of β-glucosidase production by Paecilomyces variotii and its potential application in bioassay system for boric acid determination

Characteristics of β-glucosidase production by Paecilomyces variotii and its potential application in bioassay system for boric acid determination

Ancient bayberry increased stress resistance by enriching tissue‐specific microbiome and metabolites

Regulation of pyrimidine nucleotide formation in Pseudomonas reptilivora

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