Coilin: The first 25 years

Machyna, Martin; Neugebauer, Karla M.; Staněk, David

doi:10.1080/15476286.2015.1034923

Cited by 62 publications

(96 citation statements)

References 103 publications

(140 reference statements)

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“…Remarkably, some specific proteins found in the RNA-enriched nuclear bodies mentioned above also contain disordered domains as for example coilin in Cajal bodies, 35 which was also reported to self-associate. 36 In paraspeckles, the N-and C-terminal regions of PSP1, NONO, and PSF are predicted to be disordered, 37 and in speckles, SC35 can self-associate 36 and contains a serine-arginine rich domain, 38 which like the GAR domain is a low complexity domain.…”

Section: Rna-driven Liquid-liquid Phase Separation During Nucleolus Fmentioning

confidence: 99%

Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts

Caudron-Herger

Pankert

Rippe

2016

Nucleus

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Section: Rna-driven Liquid-liquid Phase Separation During Nucleolus Fmentioning

confidence: 99%

Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts

Caudron-Herger

Pankert

Rippe

2016

Nucleus

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“…In this regard, we analyzed Cajal bodies, subnuclear organelles, and sites of snoRNA activity, 83 in primary cytotrophoblasts that are isolated from normal second-trimester or term placentas and in the equivalent cells that were purified from the placentas of women who experienced nPTB or sPE. The strategy was to visualize Cajal body foci by the immunolocalization of coilin, which serves as a scaffold for these structures and consequently, is required for their function 84 ; cytotrophoblast identity was confirmed by the immunolocalization of cytokeratin. The images are shown in Figure 5, A.…”

Section: Trophoblast Misexpression Of Noneprotein-coding Rnas In Spementioning

confidence: 99%

Preeclampsia: novel insights from global RNA profiling of trophoblast subpopulations

Gormley

Ona

Kapidzic

et al. 2017

American Journal of Obstetrics and Gynecology

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BACKGROUND:The maternal signs of preeclampsia, which include the new onset of high blood pressure, can occur because of faulty placentation. We theorized that transcriptomic analyses of trophoblast subpopulations in situ would lend new insights into the role of these cells in preeclampsia pathogenesis. OBJECTIVE: Our goal was to enrich syncytiotrophoblasts, invasive cytotrophoblasts, or endovascular cytotrophoblasts from the placentas of severe preeclampsia cases. Total RNA was subjected to global transcriptional profiling to identify RNAs that were misexpressed compared with controls. STUDY DESIGN: This was a cross-sectional analysis of placentas from women who had been diagnosed with severe preeclampsia. Gestational age-matched controls were placentas from women who had a preterm birth with no signs of infection. Laser microdissection enabled enrichment of syncytiotrophoblasts, invasive cytotrophoblasts, or endovascular cytotrophoblasts. After RNA isolation, a microarray approach was used for global transcriptional profiling. Immunolocalization identified changes in messenger RNA expression that carried over to the protein level. Differential expression of noneprotein-coding RNAs was confirmed by in situ hybridization. A 2-way analysis of variance of non-coding RNA expression identified particular classes that distinguished trophoblasts in cases vs controls. Cajal body foci were visualized by coilin immunolocalization. RESULTS: Comparison of the trophoblast subtype data within each group (severe preeclampsia or noninfected preterm birth) identified many highly differentially expressed genes. They included molecules that are known to be expressed by each subpopulation, which is evidence that the method worked. Genes that were expressed differentially between the 2 groups, in a cell-typeespecific manner, encoded a combination of molecules that previous studies associated with severe preeclampsia and those that were not known to be dysregulated in this pregnancy

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“…27,32,33 It is thought that this process is also influenced by 10 residues of the most distal end of the C-terminus, which may control N-terminus self-interaction, which may in turn regulate coilin localization, 34 CB formation and number. 35 Possible mechanisms of coilin involvement in CB assembly have recently been discussed in detail by Machyna et al 25,36 Briefly, the model suggests that CBs may be nucleated by tethering different CB components, proteins or RNAs, to a specific site (usually transcriptionally active region) inside the nucleus in a stochastic manner. [37][38][39] Presumably multiple types of RNA (such as snRNAs, snoRNAs, and/or scaRNAs) arising from separate genomic loci may act as distinct "seeds" for further nucleation of CBs via association of specific sets of proteins for particular RNA species.…”

Section: Coilin and Cb Assemblymentioning

confidence: 99%

Cajal bodies and their role in plant stress and disease responses

et al. 2016

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Cajal bodies (CBs) are distinct sub-nuclear structures that are present in eukaryotic living cells and are often associated with the nucleolus. CBs play important roles in RNA metabolism and formation of RNPs involved in transcription, splicing, ribosome biogenesis, and telomere maintenance. Besides these primary roles, CBs appear to be involved in additional functions that may not be directly related to RNA metabolism and RNP biogenesis. In this review, we assess possible roles of plant CBs in RNA regulatory pathways such as nonsense-mediated mRNA decay and RNA silencing. We also summarize recent progress and discuss new non-canonical functions of plant CBs in responses to stress and disease. It is hypothesized that CBs can regulate these responses via their interaction with poly(ADP ribose)polymerase (PARP), which is known to play an important role in various physiological processes including responses to biotic and abiotic stresses. It is suggested that CBs and their components modify PARP activities and functions.

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Coilin: The first 25 years

Cited by 62 publications

References 103 publications

Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts

Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts

Preeclampsia: novel insights from global RNA profiling of trophoblast subpopulations

Cajal bodies and their role in plant stress and disease responses

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