Coiled Structure of the Nucleocapsid of Avian Myeloblastosis Virus

Structural Studies of Avian Myeloblastosis Virus: Comparison of Polypeptides in Virion and Core Component by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

“…Core preparations from AMV had substantial amounts of the following polypeptides: p12, p27, p35, p60, p64, p91, and p98. Evidence from our results and earlier morphologic investigations (11,16,25) and biochemical studies (2, 6, 9, 10, 21, K. Stromberg and S. H. Wilson, submitted for publication) permit some conclusions to be drawn about the structural organization of AMV. A summary of the proposed location in the viral structure of several major polypeptides is illustrated in Fig.…”

“…The core component of AMV is a distinct morphologic entity (28,29) with a diameter of approximately 80 nm which consists of a shell, or intermediate layer, that surrounds an electron-dense spherule, the nucleoid, which by negative-stain electron microscopy appears as coiled strands in avian myeloblastosis virus (AMV) (16) and other RNA tumor viruses (18,25). A preliminary report (30) detected two major polypeptides corresponding to gs 1 (26,000 molecular weight) and gs 4 (14,000 molecular weight) in the nomenclature of Fleissner (9) in AMV cores.…”

Properties of a Ribonucleoprotein Particle Isolated from Nonidet P-40-Treated Rous Sarcoma Virus

“…Several electron microscopy studies of RNA tumor virus structures have presented evidence that the central RNA-containing substructure of the particle is a coiled filament (4,6,11,17). In a recent study of virion morphology, Luftig and Kilham reported the isolation of nucleoprotein strands with a buoyant density in CsCl of 1.34 g/ml from SDS-disrupted Rauscher leukemia virus (12).…”

Comprehensive Virology

“…Nonetheless, one must be cautious in accepting this as the native configuration, since disruption of the virion may confer a configuration which is merely a reflection of its form in the nucleoid. Electron micrographs from studies purporting to demonstrate a coiled-filament configuration within the virion unfortunately lack definition (Lacour et at., 1970;Nowinski et at., 1970;Luftig and Kilham, 1971;Sarkar et al, 1971). Viewing of the natural configuration probably will require methods allowing the removal of the obscuring outer envelope and intermediate layer without disturbing the nucleoid.…”