Coiled bodies in supraoptic nuclei of the rat hypothalamus during the postnatal period

Hervás, José-Pablo; Villegas, Juan; Crespo, David Revuelta; Lafarga, Miguel

doi:10.1002/aja.1001590408

Cited by 30 publications

(7 citation statements)

References 37 publications

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“…In all types of TGN, most CBs were composed of both granules and dense coiled threads and an intervening lower density matrix (Fig. 5A–E), as previously reported in other neuronal types (Kinderman and LaVelle, 1976; Hervas et al, 1980; Lafarga et al, 1983). An important structural variation was the occurrence in a few neurons of a segregated CB with a separated mass of dense fibrillar material.…”

Section: Resultssupporting

confidence: 84%

“…Regarding the ultrastructural organization of CBs, the majority of CBs in trigeminal ganglion neurons present the typical morphology of dense coiled threads (Kinderman and LaVelle, 1976; Hervas et al, 1980; Lafarga et al, 1983; Clark et al, 1990), in which coilin, fibrillarin, and snRNPs appear concentrated by immunoelectron microscopy (Raska et al, 1990; Lafarga et al, 1998; present results). However, the molecular composition of the second structural component of the CB, the intervening lower density matrix, remains to be clarified.…”

Section: Discussionmentioning

confidence: 48%

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Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

et al. 2000

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Trigeminal ganglion neurons comprise three main cell body-size types. This cell size heterogeneity provides an excellent neuronal model to study the cell size-dependent organization and dynamics of the nucleoli, Cajal (coiled) bodies (CBs), and nuclear speckles of pre-mRNA splicing factors, nuclear structures that play a key role in the normal neuronal physiology. We have analyzed the number of nucleoli and CBs and the structural and molecular organization of CBs and nuclear speckles in the three neuronal types by using immunofluorescence with antibodies that recognize nucleoli (fibrillarin), CBs (coilin), and nuclear speckles (snRNPs), confocal microscopy, and electron microscopy. Whereas the mean number of nucleoli per neuron decreases as a function of cell size, the number of CBs per cell significantly increases in large neurons in comparison with the small ones. In addition, large neurons have a higher proportion of CBs associated with the nucleolus. In all neuronal types, CBs concentrate coilin, fibrillarin, snRNPs, and the survival motor neuron protein (SMN). Immunostaining for snRNPs shows small speckle domains and extensive areas of diffuse nucleoplasmic signal in large neurons, in contrast with the large nuclear speckles found in small neurons. Furthermore, flow cytometric analysis shows that all neurons are in the range of diploid cells. These findings indicate that the fusion behavior of nucleoli, the formation of CBs and their relationships with the nucleolus, as well as the compartmentalization of the pre-mRNA splicing machinery, is related to cell body size in the trigeminal ganglion neurons. Because transcriptional activity is a basic determinant mechanism of cell size in diploid cells, we suggest that our findings reflect a distinct transcription-dependent organization of the nucleolus and splicing machinery in the three cell types of trigeminal ganglion neurons.

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Section: Resultssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 48%

Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

et al. 2000

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“…Coiled bodies were first described as accessory bodies of the nucleolus ( Ramón y Cajal 1903), and later rediscovered by electron microscopists ( Hardin et al 1969; Monneron and Bernhard 1969; Hervás et al 1980). The name “coiled body” was introduced because when this subnuclear structure is viewed with the electron microscope it resembles a tangle of coiled threads ( Monneron and Bernhard 1969).…”

mentioning

confidence: 99%

The Spinal Muscular Atrophy Disease Gene Product, Smn

Carvalho

Almeida

Calapez

et al. 1999

The Journal of Cell Biology

203

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The spliceosomal snRNAs U1, U2, U4, and U5 are synthesized in the nucleus, exported to the cytoplasm to assemble with Sm proteins, and reimported to the nucleus as ribonucleoprotein particles. Recently, two novel proteins involved in biogenesis of small nuclear ribonucleoproteins (snRNPs) were identified, the Spinal muscular atrophy disease gene product (SMN) and its associated protein SIP1. It was previously reported that in HeLa cells, SMN and SIP1 form discrete foci located next to Cajal (coiled) bodies, the so-called “gemini of coiled bodies” or “gems.” An intriguing feature of gems is that they do not appear to contain snRNPs. Here we show that gems are present in a variable but small proportion of rapidly proliferating cells in culture. In the vast majority of cultured cells and in all primary neurons analyzed, SMN and SIP1 colocalize precisely with snRNPs in the Cajal body. The presence of SMN and SIP1 in Cajal bodies is confirmed by immunoelectron microscopy and by microinjection of antibodies that interfere with the integrity of the structure. The association of SMN with snRNPs and coilin persists during cell division, but at the end of mitosis there is a lag period between assembly of new Cajal bodies in the nucleus and detection of SMN in these structures, suggesting that SMN is targeted to preformed Cajal bodies. Finally, treatment of cells with leptomycin B (a drug that blocks export of U snRNAs to the cytoplasm and consequently import of new snRNPs into the nucleus) is shown to deplete snRNPs (but not SMN or SIP1) from the Cajal body. This suggests that snRNPs flow through the Cajal body during their biogenesis pathway.

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“…The large chromatin mass (Ch) shows negligible labelling 1995). A number of studies have reported the association of coiled bodies with the nucleolus in both animal (Hervas et al 1980, Raska et al 1990, Brasch and Ochs 1992, Carmo-Fonseca et al 1993, Ochs et al 1994, Bohmann et al 1995, Janevski et al 1997) and plant (Barlow 1983, Williams et al 1983, Olmedilla et al 1997 cells. In plants they were usually referred to as karyosomes or nucleolus-associated bodies (NABs) and it has been noted that these bodies were also sometimes associated with the fibrillar portion of this organelle (Hyde 1967, Williams etal.…”

Section: Discussionmentioning

confidence: 95%

Identification of coiled bodies inBrassica napus nuclei during embryogenesis and early germination

et al. 1999

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Summary. Nucleolus-associated bodies characterize interphase nuclei of many plant species. The recent demonstration that such bodies contain small nuclear ribonucleoproteins as well as coilin clearly indicates that they belong to a larger family of nuclear structures, known as coiled bodies, that have been intensively studied in a variety of animal cell types. In a previous work, we have shown that coiled bodies were present in close association with the nudeolus in Zea mays dry seeds as well as during subsequent stages of germination. This study reveals that similar nuclear structures were also present during Brassica napus embryogenesis starting at the torpedo stage and that they were, likewise, generally located on the nucleolar surface. As in the case of Z. mays, coiled bodies were observed in cells of dry seeds as well as in those of early germinating tissues. These bodies were labelled with monoclonal antibody K121, an antibody reacting with the unique Y-terminal cap structure containing 2,2,7-trimethylguanosine that characterizes small nuclear RNAs. Owing to their intimate association with the nucleolus in all stages studied, the possibility is considered that, in these plant cells, coiled bodies are assembled on an organizer element located within this organelle. Keywords: Brassica napus; Coiled bodies; Embryogenesis;Germination; Nucleolus-associated bodies; Small nuclear RNA.Abbreviations: BSA bovine serum albumin; IgM immunoglobulin M; NAB nucleolus-associated body; NAC nucleolus-associated chromatin; PBS phosphate-buffered saline; snRNA small nuclear ribonucleic acid; snRNP small nuclear ribonucleoprotein.

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Coiled bodies in supraoptic nuclei of the rat hypothalamus during the postnatal period

Cited by 30 publications

References 37 publications

Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

The Spinal Muscular Atrophy Disease Gene Product, Smn

Identification of coiled bodies inBrassica napus nuclei during embryogenesis and early germination

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