Coidentification of
 mcr-4.3
 and
 bla
 NDM-1
 in a Clinical Enterobacter cloacae Isolate from China

Chavda, Bhakti; Lv, Jingnan; Hou, Mengyun; Chavda, Kalyan D.; Kreiswirth, Barry N.; Feng, Youjun; Chen, Liang; Yu, Fei

doi:10.1128/aac.00649-18

Cited by 44 publications

(46 citation statements)

References 30 publications

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“…CP000447), and mcr-4.3 showed 100% nucleotide identity to the sequence in the genome. These data supported the conclusion that mcr-4 and its alleles originated from Shewanella frigidimarina (6), while the identification of mcr-4.3 in A. baumannii resonated with the indication of horizontal gene transfer of mcr-4.3, according to the other two reports (14,15).…”

Section: Resultssupporting

confidence: 81%

“…We could not determine whether transfer of pAB18PR065 could confer resistance to colistin. According to the other two studies, mcr-4.3 alone did not confer phenotypic resistance to colistin (14) and showed a lack of lipid A modification function (15). Recently, a study on the action and mechanism of MCR-4 showed that revertants of mcr-4.3 (either V179G or V236F) led to its expression and conferred colistin resistance of 8 g/ml, together with the acquired function of lipid A modification (22).…”

Section: Resultsmentioning

confidence: 99%

“…mcr-4.3 was first identified on a ColE10-type plasmid in 6 sequence type 54 (ST54) Enterobacter cloacae strains isolated from humans in Singapore in 2014 (14). This gene was also found on an 8,639-bp ColE plasmid in a clinical ST84 E. cloacae strain from China (15). Here, we identified a novel A. baumannii-original plasmid, pAB18PR065, carrying mcr-4.3 in an A. baumannii strain recovered from pig feces in China.…”

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confidence: 99%

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Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Cong

Zheng

et al. 2019

Antimicrob Agents Chemother

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Here, we identified mcr-4.3 in Acinetobacter baumannii, which had not been previously observed to carry an mcr gene. The mcr-4.3-harboring A. baumannii strain AB18PR065 was isolated from pig feces from a slaughterhouse in Guangdong Province of China. The mcr-4.3-carrying pAB18PR065 is 25,602 bp in size and could not be transferred in conjugation, transformation, and electroporation experiments, as we did not find any conjugation-related genes therein. pAB18PR065 harbors two copies of type II toxin-antitoxin systems, which are functional in plasmid stabilization and maintenance. pAB18PR065 shares similarity only with one recently identified plasmid, pAb-MCR4.3 (35,502 bp), from a clinical A. baumannii strain. It is likely that the emergence of pAb-MCR4.3 was due to the insertion of an 11,386-bp, ISAba19based, composite transposon into pAB18PR065. These data indicate that mcr-4.3 was captured by an A. baumannii-original plasmid via horizontal gene transfer.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Cong

Zheng

et al. 2019

Antimicrob Agents Chemother

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“…For livestock and poultry, antibiotics usage may also varied with breeding scales and farms. In addition to mcr-1 and bla NDM−1 gene, other genotype combination were also reported from China (Kong et al, 2017;Liu et al, 2017b;Chavda et al, 2018;Wang R. et al, 2018). The mcr and bla NDM genes co-harbored isolates from China are often E.…”

Section: Discussionmentioning

confidence: 91%

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Fan

Duan

et al. 2020

Front. Microbiol.

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Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or bla NDM genes are increasing. In this study, mcr-1 and bla NDM distribution of 12,858 Gramnegative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the bla NDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the bla NDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or bla NDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and bla NDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.

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“…As well, Enterobacter spp. are increasingly being isolated in many clinical infections in which they are found to as a major host of mcr colistin resistance genes and other clinically important MDR determinants 34,[37][38][39] (Tables S1-S3), which can be transferred to other intestinal pathogens 16,17,30 . Notably, the E. coli isolates also exhibited high resistance rates against important clinical antibiotics (Tables S1-S3).…”

Section: Discussionmentioning

confidence: 99%

Phylogeography and Resistome Epidemiology of Gram-Negative Bacteria in Africa: A Systematic Review and Genomic Meta-Analysis from a One-Health Perspective

Sekyere

Reta

2020

Preprint

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Objectives/Backgrounds: Antibiotic resistance (ABR) remains a major threat to public health and infectious disease management globally. However, ABR ramifications in developing countries is worsened by limited molecular diagnostics, expensive therapeutics, inadequate skilled clinicians and scientists, and unsanitary environments. Methods: Data on specimens, species, clones, resistance genes, mobile genetic elements, and diagnostics were extracted and analysed from English articles published between 2015 and December 2019. The genomes and resistomes of the various species, obtained from PATRIC and NCBI, were analysed phylogenetically using RAxML and annotated with Figtree. The phylogeography of resistant clones/clades was mapped manually. Results & conclusion: Thirty species from 31 countries and 24 genera from 41 countries were respectively analysed from 146 articles and 3028 genomes. Genes mediating resistance to β-lactams (including blaTEM-1, blaCTX-M, blaNDM, blaIMP, blaVIM, blaOXA-48/181), fluoroquinolones (oqxAB, qnrA/B/D/S, gyrA/B and parCE mutations etc.), aminoglycosides (including armA, rmtC/F), sulphonamides (sul-1/2/3), trimethoprim (dfrA), tetracycline (tet(A/B/C/D/G/O/M/39)), colistin (mcr-1), phenicols (catA/B, cmlA), and fosfomycin (fosA) were mostly found in Enterobacter spp. and K. pneumoniae, and also in Serratia marcescens, Escherichia coli, Salmonella enterica, Pseudomonas, Acinetobacter baumannii, etc. on mostly IncF-type, IncX3/4, ColRNAI, and IncR plasmids, within IntI1 gene cassettes, insertion sequences and transposons. Clonal and multiclonal outbreaks and dissemination of resistance genes across species, countries and between humans, animals, plants and the environment were observed; E. coli ST103, K. pneumoniae ST101, S. enterica ST1/2 and V. cholerae ST69/515 were common strains. Most pathogens were of human origin and zoonotic transmissions were relatively limited. One Health studies in Africa are needed.

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Coidentification of mcr-4.3 and bla _NDM-1 in a Clinical Enterobacter cloacae Isolate from China

Cited by 44 publications

References 30 publications

Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Phylogeography and Resistome Epidemiology of Gram-Negative Bacteria in Africa: A Systematic Review and Genomic Meta-Analysis from a One-Health Perspective

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Coidentification of mcr-4.3 and bla NDM-1 in a Clinical Enterobacter cloacae Isolate from China

Cited by 44 publications

References 30 publications

Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Identification of a Novel Plasmid Carrying mcr-4.3 in an Acinetobacter baumannii Strain in China

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Phylogeography and Resistome Epidemiology of Gram-Negative Bacteria in Africa: A Systematic Review and Genomic Meta-Analysis from a One-Health Perspective

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Coidentification of mcr-4.3 and bla _NDM-1 in a Clinical Enterobacter cloacae Isolate from China