Cohort profile: Greifswald approach to individualized medicine (GANI_MED)

Grabe, Hans J.; Aßel, Heinrich; Bahls, Thomas; Dörr, Marcus; Endlich, Karlhans; Endlich, Nicole; Erdmann, Pia; Ewert, Ralf; Felix, Stephan B.; Fiene, Beate; Fischer, Tobias; Fleßa, Steffen; Friedrich, Nele; Gadebusch-Bondio, Mariacarla; Salazar, Manuela Gesell; Hammer, Elke; Häring, R.; Havemann, Christoph; Hecker, Michael; Hoffmann, Wolfgang; Holtfreter, Birte; Kacprowski, Tim; Klein, Kathleen; Kocher, Thomas; Kock, Holger; Krafczyk, Janina; Kuhn, Jana; Langanke, Martin; Lendeckel, Uwe; Lerch, Markus M.; Lieb, Wolfgang; Lorbeer, Roberto; Mayerle, Julia; Meissner, Konrad; Schwabedissen, Henriette E. Meyer zu; Nauck, Matthias; Ott, Konrad; Rathmann, Wolfgang; Rettig, Rainer; Richardt, C; Saljé, Karen; Schminke, Ulf; Schulz, Andrea; Schwab, Matthias; Siegmund, Werner; Stracke, Sylvia; Suhre, Karsten; Ueffing, Marius; Ungerer, Saskia; Völker, Uwe; Völzke, Henry; Wallaschofski, Henri; Werner, Vivian; Zygmunt, Marek; Kroemer, Heyo K.

doi:10.1186/1479-5876-12-144

Cited by 45 publications

(48 citation statements)

References 70 publications

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“…Participants were recruited in the GANI_MED nephrology cohort . All participants signed informed written consent forms.…”

Section: Methodsmentioning

confidence: 99%

“…Participants were recruited in the GANI_MED nephrology cohort. 3 All participants signed informed written consent forms. The study is consistent with the principles of the declaration of Helsinki, as reflected by an a priori approval of the Ethics Committee of the University of Greifswald.…”

Section: Study Participantsmentioning

confidence: 99%

“…Therefore, patient cohorts were recruited and investigated under standardized and routine conditions. The cohort investigated in this report consisted of patients diagnosed with chronic kidney disease (CKD) …”

Section: Introductionmentioning

confidence: 99%

“…The cohort investigated in this report consisted of patients diagnosed with chronic kidney disease (CKD). 3 CKD mostly affects risk groups like patients suffering from diabetes mellitus or hypertension. [4][5][6] The disease is characterized by a decrease and subsequently by a loss of kidney function named endstage renal disease.…”

Section: Introductionmentioning

confidence: 99%

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BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function

Endlich

Lange

Kuhn

et al. 2018

J Cellular Molecular Medi

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Podocyte loss and changes to the complex morphology are major causes of chronic kidney disease (CKD). As the incidence is continuously increasing over the last decades without sufficient treatment, it is important to find predicting biomarkers. Therefore, we measured urinary mRNA levels of podocyte genes NPHS1, NPHS2, PODXL and BDNF, KIM‐1, CTSL by qRT‐PCR of 120 CKD patients. We showed a strong correlation between BDNF and the kidney injury marker KIM‐1, which were also correlated with NPHS1, suggesting podocytes as a contributing source. In human biopsies, BDNF was localized in the cell body and major processes of podocytes. In glomeruli of diabetic nephropathy patients, we found a strong BDNF signal in the remaining podocytes. An inhibition of the BDNF receptor TrkB resulted in enhanced podocyte dedifferentiation. The knockdown of the orthologue resulted in pericardial oedema formation and lowered viability of zebrafish larvae. We found an enlarged Bowman's space, dilated glomerular capillaries, podocyte loss and an impaired glomerular filtration. We demonstrated that BDNF is essential for glomerular development, morphology and function and the expression of BDNF and KIM‐1 is highly correlated in urine cells of CKD patients. Therefore, BDNF mRNA in urine cells could serve as a potential CKD biomarker.

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“…Participants were recruited in the GANI_MED nephrology cohort . All participants signed informed written consent forms.…”

Section: Methodsmentioning

confidence: 99%

Section: Study Participantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function

Endlich

Lange

Kuhn

et al. 2018

J Cellular Molecular Medi

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“…A central data management structure has been implemented to capture and integrate all relevant clinical data for research purposes. Project design and cohort profile have been described in detail elsewhere [31]. SHIP is a population-based survey conducted in West Pomerania including the city of Greifswald.…”

Section: Methodsmentioning

confidence: 99%

Urinary Angiotensinogen and Renin Excretion are Associated with Chronic Kidney Disease

Juretzko¹,

Steinbach²,

Hannemann³

et al. 2017

Kidney Blood Press Res

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Background/Aims: Several studies sought to identify new biomarkers for chronic kidney disease (CKD). As the renal renin-angiotensin system is activated in CKD, urinary angiotensinogen or renin excretion may be suitable candidates. We tested whether urinary angiotensinogen or renin excretion is elevated in CKD and whether these parameters are associated with estimated glomerular filtration rate (eGFR). We further tested whether urinary angiotensinogen or renin excretion may convey additional information beyond that provided by albuminuria. Methods: We measured urinary and plasma angiotensinogen, renin, albumin and creatinine in 177 CKD patients from the Greifswald Approach to Individualized Medicine project and in 283 healthy controls from the Study of Health in Pomerania. The urinary excretion of specific proteins is given as protein-to-creatinine ratio. Receiver operating characteristic (ROC) curves, spearman correlation coefficients and linear regression models were calculated. Results: Urinary angiotensinogen [2,511 (196-31,909) vs. 18.6 (8.3-44.0) pmol/g, *P<0.01] and renin excretion [0.311 (0.135-1.155) vs. 0.069 (0.045-0.148) pmol/g, *P<0.01] were significantly higher in CKD patients than in healthy controls. The area under the ROC curve was significantly larger when urinary angiotensinogen, renin and albumin excretion were combined than with urinary albumin excretion alone. Urinary angiotensinogen (ß-coefficient -2.405, standard error 0.117, P<0.01) and renin excretion (ß-coefficient -0.793, standard error 0.061, P<0.01) were inversely associated with eGFR. Adjustment for albuminuria, age, sex, systolic blood pressure and body mass index did not significantly affect the results. Conclusion: Urinary angiotensinogen and renin excretion are elevated in CKD patients. Both parameters are negatively associated with eGFR and these associations are independent of urinary albumin excretion. In CKD patients urinary angiotensinogen and renin excretion may convey additional information beyond that provided by albuminuria.

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Effect of periodontal treatment on preclinical Alzheimer's disease—Results of a trial emulation approach

Schwahn

Frenzel

Holtfreter

et al. 2021

Alzheimer's & Dementia

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Introduction:We investigated the relationship between periodontal treatment and pre-clinical Alzheimer's disease (AD). Methods:In this quasi-experimental design, 177 periodontally treated patients from the "Greifswald Approach to Individualized Medicine" cohort, which used the same protocols as the population-based Study of Health in Pomerania TREND (SHIP-TREND), and 409 untreated subjects from SHIP-TREND were analyzed. Subjects were younger than 60 years at the magnetic resonance imaging examination, with a median observation period of 7.3 years. Imaging markers for brain atrophy in late-onset AD and brain aging were used as the outcomes.Results: Robust to sensitivity analyses, periodontal treatment had a favorable effect on AD-related brain atrophy (-0.41; 95% confidence interval: -0.70 to -0.12; P = .0051), which corresponds to a shift from the 50th to the 37th percentile of the outcome distribution. For brain aging, the treatment effect was uncertain. Conclusion:Periodontitis is related to pre-clinical AD in our population.

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Cohort profile: Greifswald approach to individualized medicine (GANI_MED)

Cited by 45 publications

References 70 publications

BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function

BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function

Urinary Angiotensinogen and Renin Excretion are Associated with Chronic Kidney Disease

Effect of periodontal treatment on preclinical Alzheimer's disease—Results of a trial emulation approach

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