Cohort Profile: African Collaborative Center for Microbiome and Genomics Research’s (ACCME's) Human Papillomavirus (HPV) and Cervical Cancer Study

Adebamowo, Sally N.; Dareng, Eileen; Famooto, Ayo; Offiong, Richard; Olaniyan, Olayinka; Obende, Kayode; Adebayo, Amos; Ologun, Sanni; Alabi, Bunmi; Peter, Andreas; Bakare, Rasheed; Odutola, Michael; Olawande, Oluwatoyosi; Okuma, James; Odonye, George; Adebiyi, Ruxton; Dakum, Patrick; Adebamowo, Clement

doi:10.1093/ije/dyx050

Cited by 36 publications

(34 citation statements)

References 52 publications

(35 reference statements)

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“…Previous studies have always overlooked differences in vaginal and cervical microbiota and very few studies have been done on the distinction between cervical and vaginal microbiota of hrHPV infected women in Chinese population, which has led us to design this project to explore the continuum and distinction between the cervical and vaginal microbiota in hrHPV-infected women in China. This research would be meaningful for further researches to explore the role of microbiota in cervical carcinogenesis [12] .…”

Section: Introductionmentioning

confidence: 90%

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Zhang

et al. 2020

Preprint

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Background: High-risk human papilloma virus (hrHPV) is regarded as the main causal factor of cervical precancer and cancer when persistent infection is left untreated. Previous studies have declared that HPV is associated with microecological environment of lower genital tract but haven’t discriminate vaginal microbiota from that of cervix. Objective: To analyze the distinction between vaginal and cervical microbiota in high-risk HPV(+) Chinese women. Methods: One hundred participants were recruited including 20 healthy women with HPV (-), 32 with other hrHPV (+), 38 subjects with HPV16/18 (+) and 10 with cervical carcinoma, demographics of whom were collected and analyzed. Vaginal and cervical microbiota were separately tested through next-generation sequencing technology (NGS) targeting the variable region (V3-V4) of bacterial ribosome 16S rRNA gene. Results: 1. Analysis of demographics demonstrated that hrHPV infected women tend to be accustomed to vaginal douching (p =0.001), show more frequent usage of sanitary pads (p =0.007), have more sex partners (p =0.047), be more sexually active (p =0.025), have more diverse ways of contraception (p =0.001) and history of vaginitis (p =0.002). 2. NGS identified microbial diversity of cervical microbiota was much higher than that of vagina with significantly increased Proteobacteria and decreased Lactobacillus. Variation of cervical microbiota of hrHPV(+) subjects partly similar to vaginal microbiota but had its unique features. Sphingomonas of α-Proteobacteria, almost invisible in the vagina, was more frequent at normal cervix whereas decreased remarkably at hrHPV(+) cervix. Reversely, γ-Proteobacteria showed a significant positive correlation with HPV16/18 and cervical cancer. BV related anaerobes like Gardnerella, Prevotella, Atopobium and Sneathia showed similar changes in both vaginal and cervical microbiota of hrHPV(+) women and did not exhibit cervical specificity. Conclusions: Cervical microbiota has its uniqueness from that of vagina in bacterial communities presenting a higher proportion of Proteobacteria, of which Sphingomonas is potentially predictive of a health guardian of hrHPV while Pseudomonas the opposite.

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Section: Introductionmentioning

confidence: 90%

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Zhang

et al. 2020

Preprint

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“…With support from H3Africa, infrastructures for genomics research have been set-up in several locations across Africa [10]. With proper maintenance and constant funding, these infrastructures could become well established and used to generate results that will contribute to the knowledge gap of genomics in African populations.…”

Section: Resourcesmentioning

confidence: 99%

Implementation of genomics research in Africa: challenges and recommendations

Adebamowo

Francis

Tambo

et al. 2018

Global Health Action

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Background: There is exponential growth in the interest and implementation of genomics research in Africa. This growth has been facilitated by the Human Hereditary and Health in Africa (H3Africa) initiative, which aims to promote a contemporary research approach to the study of genomics and environmental determinants of common diseases in African populations. Objective: The purpose of this article is to describe important challenges affecting genomics research implementation in Africa. Methods: The observations, challenges and recommendations presented in this article were obtained through discussions by African scientists at teleconferences and face-to-face meetings, seminars at consortium conferences and in-depth individual discussions. Results: Challenges affecting genomics research implementation in Africa, which are related to limited resources include ill-equipped facilities, poor accessibility to research centers, lack of expertise and an enabling environment for research activities in local hospitals. Challenges related to the research study include delayed funding, extensive procedures and interventions requiring multiple visits, delays setting up research teams and insufficient staff training, language barriers and an underappreciation of cultural norms. While many African countries are struggling to initiate genomics projects, others have set up genomics research facilities that meet international standards. Conclusions: The lessons learned in implementing successful genomics projects in Africa are recommended as strategies to overcome these challenges. These recommendations may guide the development and application of new research programs in low-resource settings.

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“…28 This initiative has produced 382 trainees and 219 publications. 28 While primary results from the H3Africa projects for the study of complex disease traits have not yet been published, they are poised to make substantial contributions to increasing diversity in genomic research given the following enrollment numbers: a study of over 10,000 individuals for cardiometabolic disease risk (AWI-GEN), 29,30 a study of 6000 T2D cases and 6000 controls (H3Africa T2D Study), 31 a study of kidney disease (n = 4000 cases and 4000 controls) (H3Africa Kidney Disease Research Network), 32 a study of 3000 stroke cases and 3000 controls (SIREN), 33 a study of over 11,000 women on Human Papilloma Virus and cervical cancer risk (African Collaborative Center for Microbiome and Genomics Research), 34 and a combined resource for cardiovascular studies with over 30,000 35 CAAPA, 110 NeuroGAP-Psychosis, 36 All of Us, 23 and the GWAS Catalog 10 .…”

Section: Insights Into Human Biologymentioning

confidence: 99%

Evaluating the promise of inclusion of African ancestry populations in genomics

2020

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The lack of representation of diverse ancestral backgrounds in genomic research is well-known, and the resultant scientific and ethical limitations are becoming increasingly appreciated. The paucity of data on individuals with African ancestry is especially noteworthy as Africa is the birthplace of modern humans and harbors the greatest genetic diversity. It is expected that greater representation of those with African ancestry in genomic research will bring novel insights into human biology, and lead to improvements in clinical care and improved understanding of health disparities. Now that major efforts have been undertaken to address this failing, is there evidence of these anticipated advances? Here, we evaluate the promise of including diverse individuals in genomic research in the context of recent literature on individuals of African ancestry. In addition, we discuss progress and achievements on related technological challenges and diversity among scientists conducting genomic research.

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Cohort Profile: African Collaborative Center for Microbiome and Genomics Research’s (ACCME's) Human Papillomavirus (HPV) and Cervical Cancer Study

Cited by 36 publications

References 52 publications

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Implementation of genomics research in Africa: challenges and recommendations

Evaluating the promise of inclusion of African ancestry populations in genomics

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