Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes

Krasikova, Alla; Barbero, José Luís; Gaginskaya, Elena

doi:10.1007/s10577-005-1005-6

Cited by 29 publications

(27 citation statements)

References 43 publications

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“…To detect centromere positions on chicken and quail LBCs, preparations were stained with rabbit polyclonal antibodies K828 or K854 against STAG2 and Rad21 cohesin subunits, correspondingly, as previously described (Krasikova et al 2005). After image acquisition, slides were washed in 4× SSC, 0.1 % Tween 20 at 42°C, dehydrated in 96 % ethanol, air-dried, and then used for FISH.…”

Section: Immunofluorescent Stainingmentioning

confidence: 99%

Centromere positions in chicken and Japanese quail chromosomes: de novo centromere formation versus pericentric inversions

et al. 2012

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Chicken (Gallus gallus domesticus, GGA) and Japanese quail (Coturnix coturnix japonica, CCO) karyotypes are very similar. They have identical chromosome number (2n078) and show a high degree of synteny. Centromere positions on the majority of orthologous chromosomes are different in these two species. To explore the nature of this divergence, we used highresolution comparative fluorescent in situ hybridization mapping on giant lampbrush chromosomes (LBCs) from growing oocytes. We applied 41 BAC clones specific for GGA1, 2, 3, 11, 12, 13, 14, and 15 to chicken and quail LBCs. This approach allowed us to rule out a pericentric inversion earlier proposed to explain the difference between GGA1 and CCO1. In addition to a well-established large-scale pericentric inversion that discriminates GGA2 and CCO2, we identified another, smaller one in the large inverted region. For the first time, we described in detail inversions that distinguish GGA3 from CCO3 and GGA11 from CCO11. Despite the newly identified and confirmed inversions, our data suggest that, in chicken and Japanese quail, the difference in centromere positions is not mainly caused by pericentric inversions but is instead due to centromere repositioning events and the formation of new centromeres. We also consider the formation of short arms of quail microchromosomes by heterochromatin accumulation as a third scenario that could explain the discrepancy in centromeric indexes.

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Section: Immunofluorescent Stainingmentioning

confidence: 99%

Centromere positions in chicken and Japanese quail chromosomes: de novo centromere formation versus pericentric inversions

et al. 2012

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“…Centromere protein bodies of chicken, quail and turkey lampbrush chromosomes are very small (approximately 1 μm in diameter) and look like centromere granules of amphibian lampbrush chromosomes (Krasikova et al 2006Macgregor 2012). A centromere protein body can be identified by marker components such as subunits of cohesin complex and component of synaptonemal lateral element SYCP3 (Krasikova et al 2005). However, the accurate location and specific functions of centromere protein bodies in a centromere region of avian lampbrush chromosomes are still puzzling Krasikova and Gaginskaia 2010).…”

Section: Introductionmentioning

confidence: 99%

High-resolution mapping and transcriptional activity analysis of chicken centromere sequences on giant lampbrush chromosomes

2012

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Exploration into morphofunctional organisation of centromere DNA sequences is important for understanding the mechanisms of kinetochore specification and assembly. In-depth epigenetic analysis of DNA fragments associated with centromeric nucleosome proteins has demonstrated unique features of centromere organisation in chicken karyotype: there are both mature centromeres, which comprise chromosome-specific homogeneous arrays of tandem repeats, and recently evolved primitive centromeres, which consist of non-tandemly organised DNA sequences. In this work, we describe the arrangement and transcriptional activity of chicken centromere repeats for Cen1, Cen2, Cen3, Cen4, Cen7, Cen8, and Cen11 and non-repetitive centromere sequences of chromosomes 5, 27, and Z using highly elongated lampbrush chromosomes, which are characteristic of the diplotene stage of oogenesis. The degree of chromatin packaging and fine spatial organisations of tandemly repetitive and non-tandemly repetitive centromeric sequences significantly differ at the lampbrush stage. Using DNA/RNA FISH, we have demonstrated that during the lampbrush stage, DNA sequences are transcribed within the centromere regions of chromosomes that lack centromere-specific tandem repeats. In contrast, chromosome-specific centromeric repeats Cen1, Cen2, Cen3, Cen4, Cen7, Cen8, and Cen11 do not demonstrate any transcriptional activity during the lampbrush stage. In addition, we found that CNM repeat cluster localises adjacent to non-repetitive centromeric sequences in chicken microchromosome 27 indicating that centromere region in this chromosome is repeat-rich. Cross-species FISH allowed localisation of the sequences homologous to centromeric DNA of chicken chromosomes 5 and 27 in centromere regions of quail orthologous chromosomes.

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“…(4) Finally, in birds, cohesion proteins aggregate into protein bodies that assemble adjacent to centromeres (Krasikova et al, 2005). These protein bodies vary in size among and within bird species (Krasikova et al, 2006) and it has been suggested that they may interfere with spindle attachment to the centromere (Rutkowska and Badyaev, 2008).…”

Section: Introductionmentioning

confidence: 99%

Segregation distortion in chicken and the evolutionary consequences of female meiotic drive in birds

et al. 2010

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As all four meiotic products give rise to sperm in males, female meiosis result in a single egg in most eukaryotes. Any genetic element with the potential to influence chromosome segregation, so that it is preferentially included in the egg, should therefore gain a transmission advantage; a process termed female meiotic drive. We are aware of two chromosomal components, centromeres and telomeres, which share the potential to influence chromosome movement during meioses and make the following predictions based on the presence of female meiotic drive: (1) centromere-binding proteins should experience rapid evolution as a result of a conflict between driving centromeres and the rest of the genome; and (2) segregation patterns should be skewed near centromeres and telomeres. To test these predictions, we first analyze the molecular evolution of seven centromere-binding proteins in nine divergent bird species.We find strong evidence for positive selection in two genes, lending support to the genomic conflict hypothesis. Then, to directly test for the presence of segregation distortion, we also investigate the transmission of B9000 single-nucleotide polymorphisms in 197 chicken families. By simulating fair Mendelian meioses, we locate chromosomal regions with statistically significant transmission ratio distortion. One region is located near the centromere on chromosome 1 and a second region is located near the telomere on the parm of chromosome 1. Although these observations do not provide conclusive evidence in favour of the meiotic drive/ genome conflict hypothesis, they do lend support to the hypothesis that centromeres and telomeres drive during female meioses in chicken.

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Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes

Cited by 29 publications

References 43 publications

Centromere positions in chicken and Japanese quail chromosomes: de novo centromere formation versus pericentric inversions

Centromere positions in chicken and Japanese quail chromosomes: de novo centromere formation versus pericentric inversions

High-resolution mapping and transcriptional activity analysis of chicken centromere sequences on giant lampbrush chromosomes

Segregation distortion in chicken and the evolutionary consequences of female meiotic drive in birds

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