Coherent Raman Techniques

Eesley, G. L.

doi:10.1016/b978-0-08-025058-8.50008-7

Cited by 90 publications

(135 citation statements)

References 0 publications

Supporting

Mentioning

133

Contrasting

Order By: Relevance

“…1, the CARS spectrum agrees with the Raman band profile. The characteristic dispersive lineshape of the CARS spectrum (12) can be recognized in the figure. No resonant contribution is observed from the OOD-stretch vibration of heavy water (deuterium oxide, D 2 O) in this region, permitting the use of D 2 O as a contrast agent to resolve the transient distributions of intracellular water.…”

Section: Resultsmentioning

confidence: 99%

“…The method is based on vibrational contrast in a coherent anti-Stokes Raman scattering (CARS) laser-scanning microscope (9)(10)(11). Similar to conventional Raman methods, CARS vibrational spectra uniquely characterize biological (macro-) molecules in condensed phase systems and thus provide a direct means to selectively probe specific chemical species (12,13). The contrast in CARS microscopy results from vibrational active modes of molecular compounds and does not rely on complicated and possible interfering staining procedures commonly used in fluorescence microscopy.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Real-time visualization of intracellular hydrodynamics in single living cells

Potma¹

2001

Proceedings of the National Academy of Sciences

View full text Add to dashboard Cite

Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Real-time visualization of intracellular hydrodynamics in single living cells

Potma¹

2001

Proceedings of the National Academy of Sciences

View full text Add to dashboard Cite

show abstract

“…The spectra of the wild-type nematode also exhibit a peak at the weaker asymmetric CH 2 vibration (Ϸ2,880 cm Ϫ1 ), higher for the late-(L4) than the early-stage (L1) larva, which probably is a consequence of the difference in accumulated lipid stores. In the spectrum of the feeding-deficient pha-3 mutant, the CH 2 peaks are replaced by spectral profiles of dispersive shape, typically obtained at low concentrations of the probed molecules (28) and in good accordance with the depleted lipid stores of the pha-3 mutant. With the increased accumulation of lipid stores typical for the daf-2 and -4 dauer mutants, a prominent asymmetric CH 2 peak (Ϸ2,880 cm Ϫ1 ) could be expected.…”

mentioning

confidence: 99%

Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy

Hellerer

Axäng

Brackmann

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

289

290

View full text Add to dashboard Cite

Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of lipids compared with coherent antiStokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on lipid storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-␤) signaling pathways had lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized lipid droplets in the hypodermal cells, hosting as much as 40% of the total lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the lipids from gel to liquid phase. We conclude that the degree of hypodermal lipid storage and the lipid phase can be used as a marker of lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of lipid storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.lipid metabolism ͉ nonlinear microscopy ͉ obesity

show abstract

“…[18][19][20] The stimulated Raman gain (SRG) represents one of the third-order nonlinear optical processes. 12,20,21 Through the temporal and spatial interaction of the Raman pump pulse (ωRP) and the Stokes-shifted continuum pulse (ωC), photon creation can occur at frequencies resonant with vibrational energy gap. The SRG can be defined as 12,[19][20][21] …”

mentioning

confidence: 99%

Development of Femtosecond Stimulated Raman Spectroscopy: Stimulated Raman Gain via Elimination of Cross Phase Modulation

2004

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

We have developed a new femtosecond probe technique by using stimulated Raman spectroscopy. The cross phase modulation in femtosecond time scale associated with off-resonant interaction was shown to be eliminated by integrating the transient gain/loss signal over the time delay between the Raman pump pulse and the continuum pulse. The stimulated Raman gain of neat cyclohexane was obtained to demonstrate the feasibility of the technique. Spectral and temporal widths of stimulated Raman spectra were controlled by using a narrow band pass filter. Femtosecond stimulated Raman spectroscopy was proposed as a highly useful probe in time-resolved vibrational spectroscopy.

show abstract

Coherent Raman Techniques

Cited by 90 publications

References 0 publications

Real-time visualization of intracellular hydrodynamics in single living cells

Real-time visualization of intracellular hydrodynamics in single living cells

Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy

Development of Femtosecond Stimulated Raman Spectroscopy: Stimulated Raman Gain via Elimination of Cross Phase Modulation

Contact Info

Product

Resources

About